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Research article
Xinchun Jian
Xiangya Hospital Central South University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8233-903X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) play an important role in tumor progression. However, the potential biological functions and clinical importance of Linc01234 in oral squamous cell carcinoma (OSCC) remain unclear.
Methods: We evaluated the expression profile and prognostic value of Linc01234 in OSCC tissues by RT-qPCR. Then, functional in vitro experiments were performed to investigate the effects of Linc01234 on tumor growth, migration and invasion in OSCC. Mechanistically, RT-qPCR, bioinformatic analysis and dual luciferase reporter assays were performed to identify a competitive endogenous RNA (ceRNA) mechanism involving Linc01234, miR-433-3p and PAK4.
Results: We found that Linc01234 was clearly upregulated in OSCC tissues and cell lines, and its level was positively associated with T stage, lymph node metastasis, differentiation and poor prognosis of patients with OSCC. Our results shown that Linc01234 inhibited cell proliferation and metastatic abilities in CAL27 and SCC25 cells following its knockdown. Mechanistic analysis indicated that Linc01234 may act as a ceRNA (competing endogenous RNA) of miR-433-3p to relieve the repressive effect of miR-433-3p on its target PAK4.
Conclusions: Our results indicated that Linc01234 promotes OSCC progression through the Linc01234/miR-433/PAK4 axis and might be a potential therapeutic target for OSCC.
Keywords
OSCC, Linc01234, miR-433, PAK4, ceRNA
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View the published article at BMC Cancer.
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Posted 16 Jan, 2020
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On 14 Jan, 2020
Editorial decision: Accept
On 14 Jan, 2020
No comments provided
Submission checks complete
On 07 Jan, 2020
Editorial decision: Minor revision
On 07 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 17 Dec, 2019
Editor assigned
On 13 Dec, 2019
Submission checks complete
On 12 Dec, 2019
Editor invited
On 12 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 09 Dec, 2019
Review #1 received
Received 05 Dec, 2019
Reviewers invited
Invitations sent on 02 Dec, 2019
Reviewer #1 agreed
On 02 Dec, 2019
Submission checks complete
On 11 Nov, 2019
Editor invited
On 11 Nov, 2019
Editor assigned
On 11 Nov, 2019
No comments provided
Editorial decision: Minor revision
On 15 Oct, 2019
Review #1 received
Received 13 Oct, 2019
Reviewer #2 agreed
On 01 Oct, 2019
Review #2 received
Received 01 Oct, 2019
Reviewer #1 agreed
On 28 Sep, 2019
Reviewers invited
Invitations sent on 14 Aug, 2019
Submission checks complete
On 07 Aug, 2019
Editor assigned
On 24 Jul, 2019
Editor invited
On 23 Jul, 2019
First submitted
On 19 Jul, 2019
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See the published version of this preprint at BMC Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Xinchun Jian
Xiangya Hospital Central South University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8233-903X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cancer.
Version 5
Posted 16 Jan, 2020
No community comments so far
Submission checks complete
On 14 Jan, 2020
Editorial decision: Accept
On 14 Jan, 2020
No comments provided
Submission checks complete
On 07 Jan, 2020
Editorial decision: Minor revision
On 07 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 17 Dec, 2019
Editor assigned
On 13 Dec, 2019
Submission checks complete
On 12 Dec, 2019
Editor invited
On 12 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 09 Dec, 2019
Review #1 received
Received 05 Dec, 2019
Reviewers invited
Invitations sent on 02 Dec, 2019
Reviewer #1 agreed
On 02 Dec, 2019
Submission checks complete
On 11 Nov, 2019
Editor invited
On 11 Nov, 2019
Editor assigned
On 11 Nov, 2019
No comments provided
Editorial decision: Minor revision
On 15 Oct, 2019
Review #1 received
Received 13 Oct, 2019
Reviewer #2 agreed
On 01 Oct, 2019
Review #2 received
Received 01 Oct, 2019
Reviewer #1 agreed
On 28 Sep, 2019
Reviewers invited
Invitations sent on 14 Aug, 2019
Submission checks complete
On 07 Aug, 2019
Editor assigned
On 24 Jul, 2019
Editor invited
On 23 Jul, 2019
First submitted
On 19 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cancer.
Background: Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) play an important role in tumor progression. However, the potential biological functions and clinical importance of Linc01234 in oral squamous cell carcinoma (OSCC) remain unclear.
Methods: We evaluated the expression profile and prognostic value of Linc01234 in OSCC tissues by RT-qPCR. Then, functional in vitro experiments were performed to investigate the effects of Linc01234 on tumor growth, migration and invasion in OSCC. Mechanistically, RT-qPCR, bioinformatic analysis and dual luciferase reporter assays were performed to identify a competitive endogenous RNA (ceRNA) mechanism involving Linc01234, miR-433-3p and PAK4.
Results: We found that Linc01234 was clearly upregulated in OSCC tissues and cell lines, and its level was positively associated with T stage, lymph node metastasis, differentiation and poor prognosis of patients with OSCC. Our results shown that Linc01234 inhibited cell proliferation and metastatic abilities in CAL27 and SCC25 cells following its knockdown. Mechanistic analysis indicated that Linc01234 may act as a ceRNA (competing endogenous RNA) of miR-433-3p to relieve the repressive effect of miR-433-3p on its target PAK4.
Conclusions: Our results indicated that Linc01234 promotes OSCC progression through the Linc01234/miR-433/PAK4 axis and might be a potential therapeutic target for OSCC.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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