Improved Effect of a Mitochondria-Targeted Antioxidant on Hydrogen Peroxide-Induced Oxidative Stress in Human Retinal Pigment Epithelium Cells
Background: Oxidative damage in retinal pigmented epithelium (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of TPP-Niacin against the hydrogen peroxide (H2O2)-induced oxidative stress to RPE cells.
Methods: The cellular viability, lactate dehydrogenase, reactive oxygen species (ROS), and mitochondrial function were determined in the retinal ARPE-19 cells under the treatment with H2O2 or pre-treatment with TPP-Niacin. The expression level of mitochondrial related genes and some transcription factors were assessed using real-time polymerase chain reaction (RT-PCR).
Results: TPP-Niacin significantly improved cell viability reduction, reduced ROS generation and increased the antioxidant enzymes in H2O2-treated ARPE-19 cells. Mitochondrial dysfunction from H2O2-induced oxidative stress was also significantly diminished by the TPP-Niacin treatment, reduced generation of ROS, an ameliorated reduction of mitochondrial membrane potential (MMP) and an upregulated mitochondrial associated gene. In addition, TPP-Niacin markedly enhanced the expression of transcription factors (PGC-1α and NRF2) and antioxidant associated genes (especially, HO-1 and NQO-1).
Conclusion: We proved the protective effect of TPP-Niacin against H2O2-induced oxidative stress in RPE cells. TPP-Niacin is believed to have played a protective role against mitochondrial dysfunction by up-regulating antioxidant-related genes such as PGC-1α, NRF2, HO-1 and NQO-1 in RPE cells.
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On 21 Dec, 2020
Received 21 Dec, 2020
Invitations sent on 20 Dec, 2020
On 19 Dec, 2020
On 19 Dec, 2020
On 19 Dec, 2020
On 06 Dec, 2020
Received 05 Dec, 2020
On 22 Nov, 2020
Received 11 Nov, 2020
On 09 Nov, 2020
Invitations sent on 02 Nov, 2020
On 01 Nov, 2020
On 01 Nov, 2020
On 01 Nov, 2020
Posted 26 Jun, 2020
On 06 Oct, 2020
Received 02 Oct, 2020
On 08 Sep, 2020
Received 08 Sep, 2020
On 20 Aug, 2020
Invitations sent on 26 Jun, 2020
On 25 Jun, 2020
On 25 Jun, 2020
On 24 Jun, 2020
On 21 Jun, 2020
Improved Effect of a Mitochondria-Targeted Antioxidant on Hydrogen Peroxide-Induced Oxidative Stress in Human Retinal Pigment Epithelium Cells
On 21 Dec, 2020
Received 21 Dec, 2020
Invitations sent on 20 Dec, 2020
On 19 Dec, 2020
On 19 Dec, 2020
On 19 Dec, 2020
On 06 Dec, 2020
Received 05 Dec, 2020
On 22 Nov, 2020
Received 11 Nov, 2020
On 09 Nov, 2020
Invitations sent on 02 Nov, 2020
On 01 Nov, 2020
On 01 Nov, 2020
On 01 Nov, 2020
Posted 26 Jun, 2020
On 06 Oct, 2020
Received 02 Oct, 2020
On 08 Sep, 2020
Received 08 Sep, 2020
On 20 Aug, 2020
Invitations sent on 26 Jun, 2020
On 25 Jun, 2020
On 25 Jun, 2020
On 24 Jun, 2020
On 21 Jun, 2020
Background: Oxidative damage in retinal pigmented epithelium (RPE) cells contributes to the development of age-related macular degeneration, which is among the leading causes of visual loss in elderly people. In the present study, we evaluated the protective role of TPP-Niacin against the hydrogen peroxide (H2O2)-induced oxidative stress to RPE cells.
Methods: The cellular viability, lactate dehydrogenase, reactive oxygen species (ROS), and mitochondrial function were determined in the retinal ARPE-19 cells under the treatment with H2O2 or pre-treatment with TPP-Niacin. The expression level of mitochondrial related genes and some transcription factors were assessed using real-time polymerase chain reaction (RT-PCR).
Results: TPP-Niacin significantly improved cell viability reduction, reduced ROS generation and increased the antioxidant enzymes in H2O2-treated ARPE-19 cells. Mitochondrial dysfunction from H2O2-induced oxidative stress was also significantly diminished by the TPP-Niacin treatment, reduced generation of ROS, an ameliorated reduction of mitochondrial membrane potential (MMP) and an upregulated mitochondrial associated gene. In addition, TPP-Niacin markedly enhanced the expression of transcription factors (PGC-1α and NRF2) and antioxidant associated genes (especially, HO-1 and NQO-1).
Conclusion: We proved the protective effect of TPP-Niacin against H2O2-induced oxidative stress in RPE cells. TPP-Niacin is believed to have played a protective role against mitochondrial dysfunction by up-regulating antioxidant-related genes such as PGC-1α, NRF2, HO-1 and NQO-1 in RPE cells.
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