miR-129-5p inhibits clear cell renal cell carcinoma cell proliferation, migration and invasion by targeting SPN
Objective: Our study aims to investigate the mechanism of the miR-129-5p/SPN axis in clear cell renal cell carcinoma (ccRCC), providing a novel direction for the targeted therapy of ccRCC.
Methods: Bioinformatics methods were implemented to find the differentially expressed genes (DEGs) associated with ccRCC from TCGA database. qRT-PCR was performed to detect miR-129-5p and SPN mRNA expression, while western bot was carried out for the detection of protein expression of SPN. Bioinformatics analysis was used to predict the binding sites of miR-129-5p on SPN 3’UTR, while dual-luciferase assay was conducted to verify their binding relationship. CCK-8 assay, colony formation assay, wound healing assay and Transwell assay were employed to measure ccRCC cell proliferative ability, cell formation ability, cell migratory and invasive abilities. Flow cytometry was implemented to assess cell cycle and apoptosis.
Results: miR-129-5p exhibited a significantly down-regulated expression level in ccRCC, while SPN showed a remarkably up-regulated expression level. Overexpressed miR-129-5p inhibited ccRCC cell proliferative, invasive and migratory capacities while induced cell cycle arrest in G0/G1 phase and promoted cell apoptosis. Dual-luciferase assay confirmed that there was a binding relationship between miR-129-5p and SPN. Moreover, overexpressed miR-129-5p remarkably reduced SPN expression in cancer cells, weakened the promoting effect of SPN on cell proliferation, migration, invasion and cell cycle progress, and led to enhanced cell apoptotic activity.
Conclusion: Our study proves the regulatory effect of the miR-129-5p/SPN axis in ccRCC, and provides a novel potential target for precise treatment of patients with ccRCC.
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Posted 21 Jan, 2021
Received 31 Jan, 2021
On 25 Jan, 2021
Received 25 Jan, 2021
On 08 Jan, 2021
Invitations sent on 08 Jan, 2021
On 08 Jan, 2021
On 08 Jan, 2021
On 08 Jan, 2021
On 29 Nov, 2020
Received 26 Nov, 2020
On 18 Nov, 2020
On 16 Oct, 2020
Received 16 Oct, 2020
Invitations sent on 15 Oct, 2020
On 13 Oct, 2020
On 12 Oct, 2020
On 12 Oct, 2020
On 04 Aug, 2020
Received 03 Aug, 2020
Received 02 Aug, 2020
On 01 Aug, 2020
On 28 Jul, 2020
Invitations sent on 27 Jul, 2020
On 27 Jul, 2020
On 21 Jul, 2020
On 20 Jul, 2020
On 19 Jul, 2020
On 17 Jul, 2020
miR-129-5p inhibits clear cell renal cell carcinoma cell proliferation, migration and invasion by targeting SPN
Posted 21 Jan, 2021
Received 31 Jan, 2021
On 25 Jan, 2021
Received 25 Jan, 2021
On 08 Jan, 2021
Invitations sent on 08 Jan, 2021
On 08 Jan, 2021
On 08 Jan, 2021
On 08 Jan, 2021
On 29 Nov, 2020
Received 26 Nov, 2020
On 18 Nov, 2020
On 16 Oct, 2020
Received 16 Oct, 2020
Invitations sent on 15 Oct, 2020
On 13 Oct, 2020
On 12 Oct, 2020
On 12 Oct, 2020
On 04 Aug, 2020
Received 03 Aug, 2020
Received 02 Aug, 2020
On 01 Aug, 2020
On 28 Jul, 2020
Invitations sent on 27 Jul, 2020
On 27 Jul, 2020
On 21 Jul, 2020
On 20 Jul, 2020
On 19 Jul, 2020
On 17 Jul, 2020
Objective: Our study aims to investigate the mechanism of the miR-129-5p/SPN axis in clear cell renal cell carcinoma (ccRCC), providing a novel direction for the targeted therapy of ccRCC.
Methods: Bioinformatics methods were implemented to find the differentially expressed genes (DEGs) associated with ccRCC from TCGA database. qRT-PCR was performed to detect miR-129-5p and SPN mRNA expression, while western bot was carried out for the detection of protein expression of SPN. Bioinformatics analysis was used to predict the binding sites of miR-129-5p on SPN 3’UTR, while dual-luciferase assay was conducted to verify their binding relationship. CCK-8 assay, colony formation assay, wound healing assay and Transwell assay were employed to measure ccRCC cell proliferative ability, cell formation ability, cell migratory and invasive abilities. Flow cytometry was implemented to assess cell cycle and apoptosis.
Results: miR-129-5p exhibited a significantly down-regulated expression level in ccRCC, while SPN showed a remarkably up-regulated expression level. Overexpressed miR-129-5p inhibited ccRCC cell proliferative, invasive and migratory capacities while induced cell cycle arrest in G0/G1 phase and promoted cell apoptosis. Dual-luciferase assay confirmed that there was a binding relationship between miR-129-5p and SPN. Moreover, overexpressed miR-129-5p remarkably reduced SPN expression in cancer cells, weakened the promoting effect of SPN on cell proliferation, migration, invasion and cell cycle progress, and led to enhanced cell apoptotic activity.
Conclusion: Our study proves the regulatory effect of the miR-129-5p/SPN axis in ccRCC, and provides a novel potential target for precise treatment of patients with ccRCC.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5