Three-Dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation
Background: The structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures.
Methods: The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats’ stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution.
Results: The X-ray in-line phase-contrast images of the different stages of rat gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional stomach anatomical structure images were reconstruction.
Conclusion: The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
On 27 Nov, 2020
Invitations sent on 27 Nov, 2020
On 27 Nov, 2020
On 27 Nov, 2020
On 17 Nov, 2020
On 01 Nov, 2020
Received 01 Nov, 2020
On 31 Oct, 2020
Received 31 Oct, 2020
Invitations sent on 30 Oct, 2020
On 29 Oct, 2020
On 29 Oct, 2020
On 29 Oct, 2020
Posted 01 Jul, 2020
On 30 Sep, 2020
Received 09 Aug, 2020
Received 09 Aug, 2020
On 28 Jul, 2020
Invitations sent on 28 Jul, 2020
On 28 Jul, 2020
On 30 Jun, 2020
On 29 Jun, 2020
On 29 Jun, 2020
On 29 Jun, 2020
Three-Dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation
On 27 Nov, 2020
Invitations sent on 27 Nov, 2020
On 27 Nov, 2020
On 27 Nov, 2020
On 17 Nov, 2020
On 01 Nov, 2020
Received 01 Nov, 2020
On 31 Oct, 2020
Received 31 Oct, 2020
Invitations sent on 30 Oct, 2020
On 29 Oct, 2020
On 29 Oct, 2020
On 29 Oct, 2020
Posted 01 Jul, 2020
On 30 Sep, 2020
Received 09 Aug, 2020
Received 09 Aug, 2020
On 28 Jul, 2020
Invitations sent on 28 Jul, 2020
On 28 Jul, 2020
On 30 Jun, 2020
On 29 Jun, 2020
On 29 Jun, 2020
On 29 Jun, 2020
Background: The structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures.
Methods: The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats’ stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution.
Results: The X-ray in-line phase-contrast images of the different stages of rat gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional stomach anatomical structure images were reconstruction.
Conclusion: The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7