Table 1. Characteristics of the serums used
|
|
Carcass inspection
|
WBCS50
|
ELISACS50
|
Negative
|
positive
|
Total
|
Negative
|
Negative
|
43
|
1
|
44
|
|
Positive
|
25
|
27
|
52
|
|
|
68
|
28
|
96
|
positive
|
Negative
|
9
|
2
|
11
|
|
Positive
|
16
|
71
|
87
|
|
|
25
|
73
|
98
|
Total
|
|
93
|
101
|
194
|
Table 2. Diagnostic values of recombinant antigens evaluated in singleton and of Elisa-CS50
|
Western blot (gold standard)
|
Carcass inspection (gold standard)
|
Tests
|
Sensitivity (%)
|
Specificity (%)
|
Concordance (%)
|
PLR
|
Sensitivity (%)
|
Specificity (%)
|
Concordance (%)
|
PLR
|
(CI 95%)
|
(CI 95%)
|
(CI 95%)
|
(CI 95%)
|
Elisa-CS50
|
88.8
|
45.8
|
67.52
|
1.63
|
97
|
55.9
|
77.3
|
2.2
|
(80.8 – 93.7)
|
(36.2 -55.8)
|
(91.2 - 99.3)
|
(45.8 - 65.6)
|
Elisa-T14
|
80.6
|
43.8
|
62.37
|
1.43
|
88.1
|
52.7
|
71.1
|
1.9
|
(71.6 – 87.3)
|
(34.3 – 53.7)
|
(80.2 - 93.2)
|
(42.6 - 62.5)
|
Elisa-T18
|
88.8
|
37.5
|
63.4
|
1.42
|
96
|
46.2
|
72.2
|
1.8
|
(80.8 – 93.7)
|
(28.5 - 47.5)
|
(89.8 - 98.7)
|
(36.5 - 56.3)
|
Elisa-R93
|
43.9
|
60.4
|
52.06
|
1.1
|
48.5
|
65.6
|
56.7
|
1.4
|
(34.5 - 53.8)
|
(50.4 - 69.6)
|
(39 - 58.1)
|
(55.4 - 74.4)
|
Elisa-R914
|
58.2
|
48.5
|
52.06
|
1.07
|
65.3
|
53.8
|
59.8
|
1.4
|
(48.3 - 67.4)
|
(36.2 - 55.8)
|
(55.6 - 73.9)
|
(43.7 - 63.5)
|
Elisa-R915
|
58.2
|
65.6
|
61.85
|
1.69
|
63.4
|
72
|
67.5
|
2.3
|
(48.3 - 67.4)
|
(55.7 - 74.3)
|
(53.6 - 72.1)
|
(62.2 - 80.1)
|
CI: Confidence interval; PLR: positive likelihood ratio
Table 3. Diagnostic values of recombinant antigens duplex combined
C=Cumulative; C1=T14+T18, C2= T14+R93, C3= T14+R915, C4= T14+R915, C5= T18+R93, C6 = T18+R914, C7 = T18+R915, C8 = R93+R914, C9= R93+R915,
C10= R914+R915.A = Alternative; A1=T14+T18, A2= T14+R93, A3= T14+R914, A4= T14+R915, A5= T18+R93, A6= T18+R914, A7= T18+R915, A8= R93+R914,
A9= R93+R915, A10 = R914+R915 PLR: positive likelihood ratio
Table 4. Diagnostic values of recombinant antigens triplex combined
Western Blot (gold standard)
|
Carcass Inspection (gold standard)
|
Combination
|
Sensitivity (%)
|
Specificity (%)
|
Concordance(%)
|
PLR
|
Sensibility (%)
|
Specificity (%)
|
Concordance(%)
|
PLR
|
|
C11
|
84.7
|
(76.1 - 90.6)
|
40.6
|
(31 – 50.6)
|
62.88
|
1.43
|
93.1
|
(86.1 - 96.9)
|
50.5
|
(40.6 - 60.5)
|
72.7
|
1.9
|
|
C12
|
84.7
|
(76.1 - 90.6)
|
40.6
|
(31 – 50.6)
|
62.88
|
1.43
|
93.1
|
(86.1 - 96.9)
|
50.5
|
(40.6 - 60.5)
|
72.7
|
1.9
|
|
C13
|
85.7
|
(77.3 – 91.4)
|
40.6
|
(31.4 – 50.6)
|
63.4
|
1.44
|
94.1
|
(87.3 - 97.5)
|
50.5
|
(40.6 - 60.5)
|
73.2
|
1.9
|
|
C14
|
60.2
|
(50.3 – 69.3)
|
52.1
|
(42.2 – 61.8)
|
56.18
|
1.26
|
65.3
|
(55.6 - 73.9)
|
58.1
|
(47.9 - 67.6)
|
61.9
|
1.5
|
|
C15
|
63.5
|
(53.5 – 72.5)
|
60.2
|
(50.3 – 69.3)
|
61.85
|
1.6
|
66.3
|
(56.6 - 74.8)
|
71
|
(61 - 79.2)
|
68.6
|
2.3
|
|
C16
|
66.3
|
(56.5 – 74.9)
|
55.2
|
(45.2 – 64.8)
|
60.82
|
1.48
|
74.3
|
(64.9 - 81.8)
|
64.5
|
(54.36 - 73.5)
|
69.6
|
2.1
|
|
C17
|
61.2
|
(51.3 – 70.3)
|
49
|
(39.2 – 58.8)
|
55.15
|
1.2
|
66.3
|
(56.6 - 74.8)
|
54.8
|
(44.7 - 64.5)
|
60.8
|
1.5
|
|
C18
|
61.2
|
(51.3 – 70.3)
|
61.5
|
(51.4 – 70.6)
|
60.31
|
1.59
|
67.3
|
(57.6 - 75.7)
|
68.8
|
(58.8 - 77.3)
|
68
|
2.1
|
|
C19
|
68.4
|
(58.6 – 76.7)
|
52.1
|
(42.2 – 61.8)
|
60.31
|
1.43
|
76.2
|
(67 - 83.5)
|
61.3
|
(51.1 - 70.5)
|
69.1
|
2
|
|
C20
|
50
|
(40.3 – 59.7)
|
60.4
|
(50.4 – 69.6)
|
55.15
|
1.26
|
57.4
|
(47.7 - 66.6)
|
68.8
|
(58.8 - 77.3)
|
62.9
|
1.8
|
|
A11
|
94.9
|
(88.2 – 98)
|
28.1
|
(28.1 – 37.9)
|
61.85
|
1.32
|
98
|
(92.5 - 99.8)
|
32.3
|
(23.6 - 42.3)
|
66.5
|
1.4
|
|
A12
|
93.9
|
(86.9 – 97.5)
|
24
|
(16.5 – 33.5)
|
59.28
|
1.23
|
99
|
(94 - 100)
|
30.1
|
(21.7 - 40.1)
|
66
|
1.4
|
|
A13
|
93.9
|
(86.9 – 97.4)
|
31.3
|
(22.9 – 41.1)
|
62.89
|
1.36
|
98
|
(92.5 - 99.8)
|
36.6
|
(27.5 - 46.7)
|
68.6
|
1.5
|
|
A14
|
91.8
|
(84.4 – 96)
|
24
|
(16.5 – 33.5)
|
58.25
|
1.21
|
97
|
(91.2 - 99.3)
|
30.1
|
(21.7 - 40.1)
|
64.9
|
1.4
|
|
A15
|
91.8
|
(84.4– 96)
|
29.2
|
(21 – 39)
|
60.82
|
1.3
|
96
|
(89.8 - 98.7)
|
34.4
|
(25.6 - 44.5)
|
66.5
|
1.5
|
|
A16
|
91.8
|
(84.4– 96)
|
22.9
|
(15.9 – 32.4)
|
57.73
|
1.19
|
98
|
(92.5 - 99.8)
|
30.1
|
(21.7 - 40.1)
|
65.5
|
1.4
|
|
A17
|
94.9
|
(88.2 – 98)
|
22.9
|
(15.6 – 32.4)
|
59.28
|
1.23
|
99
|
(94 - 100)
|
28
|
(19.8 - 37.9)
|
64.9
|
1.4
|
|
A18
|
94.9
|
(88.2 – 98)
|
26
|
(18.3 – 35.7)
|
60.82
|
1.28
|
98
|
(92.5 - 99.8)
|
30.1
|
(21.7 - 40.1)
|
65.5
|
1.4
|
|
A19
|
93.9
|
(86.9 – 97.4)
|
21.9
|
(14.8 – 31.2)
|
58.25
|
1.2
|
99
|
(94 - 100)
|
28
|
(19.8 - 37.9)
|
64.9
|
1.4
|
|
A20
|
71.4
|
(61.7 - 79.4)
|
38.5
|
(29.4 - 48.6)
|
55.15
|
1.16
|
77.2
|
(68 - 84.3)
|
45.2
|
(35.4 - 55.3)
|
61.9
|
1.4
|
|
C=Cumulative, C11=T14+T18+R93, C12=T14+T18+R914, C13=T14+T18+R915, C14=T14+R93+R914, C15=T14+R93+R915, C16=T14+R914+R915,
C17= T18+R93+R914, C18 = T18+R93+R914, C19 = T18+R914+R915, C20 = R93+R914+R915.
A=Alternative, A11=T14+T18+R93, A12=T14+T18+R914, A13 = T14+T18+R915, A14 = T14+R93+R914, A15 = T14+R93+R915, A16 = T14+R914+R915,
A1 = T18+R93+R914, A18 = T18+R93+R914, A19 = T18+R914+R915, A20 = R93+R914+R915. PLR: positive likelihood ratio
Tableau 5. Level of agreement between combination results and carcass inspection
|
|
Carcass inspection
|
|
|
Pos
|
Neg
|
FNF(%)
|
FPF(%)
|
K
|
A2
|
Pos
|
96
|
58
|
5
|
62,4
|
0,333
|
Neg
|
5
|
35
|
A3
|
Pos
|
98
|
63
|
3
|
67,7
|
0,301
|
Neg
|
3
|
30
|
A4
|
Pos
|
97
|
54
|
4
|
58,1
|
0,344
|
Neg
|
3
|
30
|
A5
|
Pos
|
99
|
63
|
2
|
67,7
|
0,311
|
Neg
|
2
|
30
|
A6
|
Pos
|
100
|
65
|
1
|
69,9
|
0,299
|
Neg
|
1
|
28
|
A7
|
Pos
|
99
|
59
|
2
|
63,4
|
0,354
|
Neg
|
2
|
34
|
Pos = positive, Neg =negative, FNF = false negative fraction, FPF = false positive fraction,
K = Cohen’s kappa coefficient.