According to the distinct phenotypic and phylogenetic characteristics described above, strain Ai-910T is considered a novel species of a new genus within the family Marinilabiliaceae, for which the name Xiashengella succiniciproducens gen. nov., sp. nov. was proposed. And detailed description of the proposed new genus and novel species was present as below.
Description of Xiashengella gen. nov.
Xiashengella (Xia.sheng.el’la. N.L. fem. n. Xiashengella, named after Xiasheng Gu, a renowned Chinese environmental-microbiologist and engineer in wastewater treatment).
Cells are Gram-stain-negative, non-spore-forming rods. Chemoheterotrophic and strictly anaerobic. Mesophilic and alkaliphilic. The major respiratory quinone is MK-7. Produce orange pigments. Major cellular fatty acids are branched fatty acids (iso-C15: 0, anteiso-C15: 0). Major polar lipids are phosphatidylethanolamine (PE), unidentified aminophospholipid (APL) and four unknown polar lipids. The G + C content of the genomic DNA is around 42.8 mol%. The genus is affiliated to the family Marinilabiliaceae. Related members of the genus could be determined according to 16S rRNA and genome-based phylogeny. The type species is Xiashengella succiniciproducens.
Description of Xiashengella succiniciproducens sp. nov.
Xiashengella succiniciproducens (suc.ci.ni.ci.pro.du’cens. N.L. neut. n. acidum succinicum, succinic acid; L. pres. part. producens, producing; N.L. part. adj. succiniciproducens, succinic acid producing).
Colonies on mDSMZ-287 agar are orange, circular, and swollen with a smooth surface and neat edge, with a diameter of about 0.5-1 mm. Cells are slender rod-shaped (0.2–0.4 × 3.0–12.0 µm), Gram-stain-negative, non-spore-forming, move by gliding motility, with a coccoid cyst-like structure at one end, and catalase-positive, oxidase-negative. Growth occurs at 25–42°C (optimal, 38°C), pH 5.5–10.5 (optimal, 8.5). 0–2.0% (w/v) NaCl, and optimal growth at 0.06% (w/v) NaCl. Does not produce indole. No urease activity is detected. Does not reduce nitrate. Positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), acid phosphatase, naphthol-AS-BI-phosphohydrolase, α-glucosidase, β-glucosidase and α-arabinosidase, and weekly positive for arginine dihydrolase, α-galactosidase, β-glucuronidase. Acids are produced by fermenting D-glucose, D-mannitol, D-lactose, D-saccharose, D-maltose, D-xylose, D-mannose, D-sorbitol, L-rhamnose, and D-trehalose. Esculin can be hydrolyzed. Utilize substrates of amygdalin, arbutin, D-cellobiose, α-cyclodextrin, β-cyclodextrin, dextrin, D-fructose, L-fucose, D-galactose, D-galacturonic acid, gentiobiose, D-gluconic acid, D-glucosaminic acid, α-D-glucose, α-D-lactose, lactulose, maltose, maltotriose, D-mannose, D-melibiose, 3-melthyl-D-glucose, β-methyl-D-galactoside, palatinose, D-raffinose, L-rhamnose, salicin, D-sorbitol, sucrose, D-trehalose, turanose, glyoxylic acid, α-ketovaleric acid, D-malic acid, pyruvic acid, pyruvic acid methyl ester, succinamic acid, succinic acid, succinic acid mono-methyl ester, m-tartaric acid, L-Alanine, L-alanyl-L-glutamine, L-alanyl-L-histidine, L-alanyl-L-threonine, L-asparagine, L-glutamic acid, glycyl-L-aspartic acid, glycyl-L-glutamine, glycyl-L-methionine, L-serine, 2’-deoxy adenosine, thymidine, and uridine. The major end product of xylose (glucose) fermentation is succinic acid. Resistant to gentamicin, streptomycin, bacitracin. The major fatty acids are iso-C15: 0 and anteiso-C15: 0, the respiratory quinone is MK-7, the major polar lipids are phosphatidylethanolamine (PE), unidentified aminophospholipid (APL), and four unknown polar lipids. The G + C content of the genomic DNA of the type strain is 42.8 mol%.
The type strain, Ai-910T (= CGMCC 1.17893T = KCTC 25304T), was isolated from an anaerobic digestion tank. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and genome sequence of Xiashengella succiniciproducens strain Ai-910T are OM568948 and CP098400, respectively.