In order to better design the Handling-assisted exoskeleton, it is necessary to analyze the biomechanics of humane handling movements. In this study, Anybody Modeling System(AMS) simulation was used to analyze the movement state of muscles during humane handling. Combined with surface electromyography(sEMG) experiments, specific analysis and verification were carried out to obtain the position of muscles that the human body needs to assist during handling. In this study, the simulation and experiment were carried out for the manual handling process. The treatment group and the experimental group were set up. This study found that the vastus medialis muscle, vastus lateralis muscle, latissimus dorsi muscle, trapezius muscle, deltoid muscle and triceps brachii muscle needs more power in the process of handling, and it is reasonable and effective to combine sEMG signals with simulation of the musculoskeletal model to analyze the muscle condition of humane movement.