Biomimetic dressings are considered as one of the ideal medical dressings to combat bacterial infections, shorten the tissue regeneration process, and facilitate wound repair. Researchers are committed to developing a new generation of wound dressings with unique functions to promote wound repair and healing. Here, a strategy for composite nanofiber membrane construction based on natural biopolymers HA, Gel and PLGA with carrying vascular endothelial growth factor (VEGF) was provided for pro-angiogenic wound repair. The composite nanofiber membrane prepared by electrospinning technology can maintain its biomimetic properties and biochemical properties of the functional factors. The enhanced mechanical properties and flexibility enable it to fit the wound and have great biocompatibility. In addition, the highly efficiently loaded VEGF can be controllable released and targeted to the wound area, thereby promoting the exchange of nutrients and oxygen, proliferation and migration of endothelial cells, and the formation of vascular lumen, effectively accelerating tissue repair and wound healing while avoiding unnecessary drug diffusion. Therefore, composite nanofiber dressing of VEGF@PAG can break through the limitations of traditional patches and become ideal candidates for wound healing and related biomedical applications.