In the bio-molecular field of Liquid-crystalline, the stacked lipid bilayers phase represents an extensive issue. It was proven that in addition to the typical smectic order, multi-component multilayer membranes could present columnar order arising from the coupling of two-dimensional intralayer phase separation. Interlayer smectic ordering distributes across hundreds of membrane lamellae, resulting in long-range alignment of phase-separated domains. In the current paper, the self-ordering of the sample over time is investigated by dynamic speckle pattern analysis. Temporal alteration of the sample is associated with changes in the intensity and contrast of the speckle pattern. We have used different factors, including motion history, co-occurrence, and time history speckle pattern, to examine the structure evolution of lipid mixtures, which are done by sequence images of speckle pattern over time. Roughness parameters inclusive of skewness and kurtosis of speckle pattern were investigated that are related to the domain size evolution of the sample. It is shown that the activity is decreasing by increasing the cholesterol content and inertia moment is inversely proportional with the growth of domain formation. The experimental setup, data recording, and data analysis are presented too.