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Shortened red blood cell age in patients with end-stage renal disease receiving haemodialysis: a cross-sectional study
Koichiro Matsumura
Kansai Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9089-3505
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Nephrology.
Background: Cause of anaemia in end-stage renal disease is not only due to relative deficiency in erythropoietin production, but also to the complex clinical conditions. We sought to investigate the underlying mechanisms of anaemia in patients with end-stage renal disease undergoing maintenance dialysis by measuring erythrocyte creatine.
Methods: In a cross-sectional study, we evaluated 69 patients with end-stage renal disease receiving haemodialysis (n = 55) or peritoneal dialysis (n = 14). Erythrocyte creatine, a quantitative marker of mean red blood cell (RBC) age, was measured.
Results: Mean RBC age was significantly shorter in haemodialysis patients than in those with peritoneal dialysis (47.7 days vs. 59.8 days, p<0.0001), although haemoglobin levels were comparable between the groups. Spearman correlation coefficient analysis revealed that transferrin saturation (r = 0.54), ferritin (r= 0.47), and haptoglobin (r = 0.39) were positively, whereas reticulocyte (r = -0.36), weekly dose of erythropoiesis-stimulating agent (r = -0.62), erythropoietin resistance index (r = -0.64), and intradialytic ultrafiltration rate (r = -0.32) were inversely related to shortened RBC age.
Conclusions: Shortened RBC age was observed in patients receiving maintenance haemodialysis. Shortened RBC age was associated with iron deficiency, greater haptoglobin consumption, higher ESA requirements, and poor erythropoietin responsiveness as well as greater intradialytic fluid extraction.
Keywords
Anaemia, haemolysis, haemodialysis, red blood cell age
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CURRENT STATUS: Published
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On 26 Aug, 2020
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On 25 Aug, 2020
Editor invited
On 25 Aug, 2020
Version 2
Posted 22 Aug, 2020
No comments provided
Editorial decision: Minor revision
On 20 Aug, 2020
Editor assigned
On 18 Aug, 2020
Submission checks complete
On 17 Aug, 2020
Editor invited
On 17 Aug, 2020
No comments provided
Review #2 received
Received 18 Jul, 2020
Editorial decision: Major revision
On 18 Jul, 2020
Reviewer #2 agreed
On 18 Jul, 2020
Review #1 received
Received 16 Jul, 2020
Reviewers invited
Invitations sent on 13 Jul, 2020
Reviewer #1 agreed
On 13 Jul, 2020
Editor assigned
On 02 Jul, 2020
Submission checks complete
On 01 Jul, 2020
Editor invited
On 01 Jul, 2020
First submitted
On 30 Jun, 2020
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Subject Areas
Urology & Nephrology
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This preprint is under consideration at BMC Nephrology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Shortened red blood cell age in patients with end-stage renal disease receiving haemodialysis: a cross-sectional study
Koichiro Matsumura
Kansai Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9089-3505
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
No comments provided
Editor assigned
On 26 Aug, 2020
Submission checks complete
On 25 Aug, 2020
Editor invited
On 25 Aug, 2020
Version 2
Posted 22 Aug, 2020
No comments provided
Editorial decision: Minor revision
On 20 Aug, 2020
Editor assigned
On 18 Aug, 2020
Submission checks complete
On 17 Aug, 2020
Editor invited
On 17 Aug, 2020
No comments provided
Review #2 received
Received 18 Jul, 2020
Editorial decision: Major revision
On 18 Jul, 2020
Reviewer #2 agreed
On 18 Jul, 2020
Review #1 received
Received 16 Jul, 2020
Reviewers invited
Invitations sent on 13 Jul, 2020
Reviewer #1 agreed
On 13 Jul, 2020
Editor assigned
On 02 Jul, 2020
Submission checks complete
On 01 Jul, 2020
Editor invited
On 01 Jul, 2020
First submitted
On 30 Jun, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Urology & Nephrology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Nephrology.
Background: Cause of anaemia in end-stage renal disease is not only due to relative deficiency in erythropoietin production, but also to the complex clinical conditions. We sought to investigate the underlying mechanisms of anaemia in patients with end-stage renal disease undergoing maintenance dialysis by measuring erythrocyte creatine.
Methods: In a cross-sectional study, we evaluated 69 patients with end-stage renal disease receiving haemodialysis (n = 55) or peritoneal dialysis (n = 14). Erythrocyte creatine, a quantitative marker of mean red blood cell (RBC) age, was measured.
Results: Mean RBC age was significantly shorter in haemodialysis patients than in those with peritoneal dialysis (47.7 days vs. 59.8 days, p<0.0001), although haemoglobin levels were comparable between the groups. Spearman correlation coefficient analysis revealed that transferrin saturation (r = 0.54), ferritin (r= 0.47), and haptoglobin (r = 0.39) were positively, whereas reticulocyte (r = -0.36), weekly dose of erythropoiesis-stimulating agent (r = -0.62), erythropoietin resistance index (r = -0.64), and intradialytic ultrafiltration rate (r = -0.32) were inversely related to shortened RBC age.
Conclusions: Shortened RBC age was observed in patients receiving maintenance haemodialysis. Shortened RBC age was associated with iron deficiency, greater haptoglobin consumption, higher ESA requirements, and poor erythropoietin responsiveness as well as greater intradialytic fluid extraction.
Figure 1
Figure 2