Purpose
In this study, iodine stimulated Nthyori3-1 cell was used as the research object to explore the relationship between high iodine stimulated thyroid cells and ferroptosis, and further clarify its pathogenesis. This study will help to provide new clues for the treatment of thyroid related diseases.
Methods
Intervene human normal thyroid cells with iodine. The levels of apoptosis and proliferation were detected by CCK-8 and MDA. The protein levels of Nrf2、HO-1、p-ERK1 and GPX4 were determined by western blotting. Flow cytometry was performed to detect ROS production.
Results
Iodine induces ferroptosis in Nthyori3-1 cells, and the application of the inhibitor ferroptosis-1 can reverse the ferroptosis state of cells. Nrf2 / HO-1 / ERK pathway is involved in the pathogenesis of thyroid cells induced by high iodine.
Conclusion
High iodine induces ferroptosis in Nthyori3-1 thyroid cells. The addition of ferroptosis inhibitor ferroptosis-1 partially reversed the ferroptosis of cells. High iodine is involved in the regulation of Nrf2 / HO-1 / ERK signaling pathway and may be related to ferroptosis.