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Virulence Characteristics and Molecular Epidemiology of Pyogenic Liver Abscess Causing Multidrug Resistant Klebsiella pneumoniae in Wenzhou, China
Tieli Zhou
Wenzhou Medical University First Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2171-4710
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: To date, little is known about the virulence characteristics of pyogenic liver abscess (PLA) that cause multidrug resistant (MDR) Klebsiella pneumoniae (K. pneumoniae), and this might be due to that these strains are rare. This study aimed to analyze the virulence characteristics and molecular epidemiology of 12 MDR strains obtained from 163 PLA cases in a tertiary teaching hospital from the perspective of clinical characteristics, virulence phenotypes, and genotypes.
Results: The results of growth curves, string test, capsular quantification, serum killing test, biofilm formation assay, and infection model revealed that the virulence phenotypes of the 12 PLA-causing MDR K. pneumoniae were similar or even more obvious than those of typical hypervirulent K. pneumoniae strains. These MDR strains were mainly non-K1/K2 serotypes and carried multiple virulence genes. The results of multilocus sequence typing (MLST) illustrated that the MDR strains were categorized into 9 sequence types.
Conclusions: This study is the first to analyze the virulence characteristics in PLA-causing MDR strains. Our data exhibited the coexistence of hypervirulence and MDR in PLA-causing MDR K. pneumoniae strains, and the clones of those PLA-causing MDR strains were diverse and scattered. This study first found one ST11 carbapenem-resistant hypervirulent strain in PLA.
Keywords
pyogenic liver abscess, multidrug resistance, Klebsiella pneumoniae, virulence characteristics, molecular epidemiology
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CURRENT STATUS: Under Review
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Posted 20 Jan, 2021
No community comments so far
Review #1 received
Received 21 Feb, 2021
Reviewers invited
Invitations sent on 17 Feb, 2021
Reviewer #1 agreed
On 17 Feb, 2021
Editor assigned
On 13 Jan, 2021
Submission checks complete
On 13 Jan, 2021
Editor invited
On 13 Jan, 2021
No comments provided
Editorial decision: Major revision
On 16 Dec, 2020
Review #2 received
Received 06 Dec, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Review #1 received
Received 08 Nov, 2020
Reviewers invited
Invitations sent on 28 Oct, 2020
Reviewer #1 agreed
On 28 Oct, 2020
Editor assigned
On 23 Oct, 2020
Submission checks complete
On 22 Oct, 2020
Editor invited
On 22 Oct, 2020
No comments provided
Editorial decision: Major revision
On 28 Sep, 2020
Review #3 received
Received 18 Aug, 2020
Review #1 received
Received 06 Aug, 2020
Review #2 received
Received 06 Aug, 2020
Reviewer #3 agreed
On 31 Jul, 2020
Reviewer #2 agreed
On 22 Jul, 2020
Reviewer #1 agreed
On 21 Jul, 2020
Reviewers invited
Invitations sent on 20 Jul, 2020
Editor assigned
On 12 Jul, 2020
Submission checks complete
On 11 Jul, 2020
Editor invited
On 11 Jul, 2020
First submitted
On 09 Jul, 2020
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This preprint is under consideration at BMC Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Virulence Characteristics and Molecular Epidemiology of Pyogenic Liver Abscess Causing Multidrug Resistant Klebsiella pneumoniae in Wenzhou, China
Tieli Zhou
Wenzhou Medical University First Affiliated Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2171-4710
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 3
Posted 20 Jan, 2021
No community comments so far
Review #1 received
Received 21 Feb, 2021
Reviewers invited
Invitations sent on 17 Feb, 2021
Reviewer #1 agreed
On 17 Feb, 2021
Editor assigned
On 13 Jan, 2021
Submission checks complete
On 13 Jan, 2021
Editor invited
On 13 Jan, 2021
No comments provided
Editorial decision: Major revision
On 16 Dec, 2020
Review #2 received
Received 06 Dec, 2020
Reviewer #2 agreed
On 28 Nov, 2020
Review #1 received
Received 08 Nov, 2020
Reviewers invited
Invitations sent on 28 Oct, 2020
Reviewer #1 agreed
On 28 Oct, 2020
Editor assigned
On 23 Oct, 2020
Submission checks complete
On 22 Oct, 2020
Editor invited
On 22 Oct, 2020
No comments provided
Editorial decision: Major revision
On 28 Sep, 2020
Review #3 received
Received 18 Aug, 2020
Review #1 received
Received 06 Aug, 2020
Review #2 received
Received 06 Aug, 2020
Reviewer #3 agreed
On 31 Jul, 2020
Reviewer #2 agreed
On 22 Jul, 2020
Reviewer #1 agreed
On 21 Jul, 2020
Reviewers invited
Invitations sent on 20 Jul, 2020
Editor assigned
On 12 Jul, 2020
Submission checks complete
On 11 Jul, 2020
Editor invited
On 11 Jul, 2020
First submitted
On 09 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: To date, little is known about the virulence characteristics of pyogenic liver abscess (PLA) that cause multidrug resistant (MDR) Klebsiella pneumoniae (K. pneumoniae), and this might be due to that these strains are rare. This study aimed to analyze the virulence characteristics and molecular epidemiology of 12 MDR strains obtained from 163 PLA cases in a tertiary teaching hospital from the perspective of clinical characteristics, virulence phenotypes, and genotypes.
Results: The results of growth curves, string test, capsular quantification, serum killing test, biofilm formation assay, and infection model revealed that the virulence phenotypes of the 12 PLA-causing MDR K. pneumoniae were similar or even more obvious than those of typical hypervirulent K. pneumoniae strains. These MDR strains were mainly non-K1/K2 serotypes and carried multiple virulence genes. The results of multilocus sequence typing (MLST) illustrated that the MDR strains were categorized into 9 sequence types.
Conclusions: This study is the first to analyze the virulence characteristics in PLA-causing MDR strains. Our data exhibited the coexistence of hypervirulence and MDR in PLA-causing MDR K. pneumoniae strains, and the clones of those PLA-causing MDR strains were diverse and scattered. This study first found one ST11 carbapenem-resistant hypervirulent strain in PLA.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6