Index of the application for a binocular visual system. According to the measurement results of a binocular vision system is mainly used relative measuring accuracy to verify, but that can't be achieved to verify by artificial specific measurement, and also this work is measured in camera coordinate system, because of the camera's optical center couldn't be located, for the problem that where is the specific camera coordinate system, that can't give a specific location, but only giving the definition of a concept. In order to solve these problems, a calibration transformation method of the camera coordinate system based on the least square method is proposed, this method mainly through calibration of the camera coordinate system transforms to a presetting specific existence and positioning coordinate system. Meanwhile, by making the optical axis through the geometric center of a camera lens, then the accuracy of the binocular vision measurement system can be verified in the coordinate system. Finally, the result of the experiment shows that the proposed method is correct and effective to calibrate camera coordinate system, the accuracy of calibration is more higher, and the requiring equipment for verification is very simple.