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Research article
Comparison of Cytokine Levels in the Aqueous Humor of Polypoidal Choroidal Vasculopathy and Neovascular Age-Related Macular Degeneration Patients
Youxin Chen
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7231-5058
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Ophthalmology.
Background
The concentrations of cytokines in the aqueous humor from neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV) may vary. The study was conducted to compare various cytokine levels in the aqueous humor of eyes with PCV, nAMD and control.
Methods
The present case control study included 49 treatment-naïve eyes from 49 patients (PCV 24, nAMD 11, and cataract 14 eyes). Totally 34 angiogenic and inflammatory cytokines in the aqueous humor were measured by Luminex bead-based multiplex array.
Results
After adjusting for gender and age by multivariate logistic analysis, concentrations of IL-31, LIF, SDF1-α, VEGF-A, VEGF-D were significantly higher in eyes with nAMD or PCV compared with control eyes (all P<0.05, times in nAMD: 59.5, 6.0, 7.0, 4.5, 5.6, respectively, times in PCV: 51.9, 5.21, 6.6, 4.0, 5.1, respectively ), and concentrations of HGF, IP-10, MCP-1, IL-13 were significantly lower in eyes with nAMD or PCV than in control eyes (all P<0.05, times in nAMD: 2.6, 2.0, 4.5, 4.7, respectively, times in PCV: 1.9, 3.0, 3.0, 2.8, respectively), but none of the 34 cytokines, including VEGF and IL-8, showed significantly different between eyes with nAMD and PCV.
Conclusions
Various cytokines involved in inflammation and angiogenesis including elevated IL-31, LIF, SDF1-α, VEGF-A, VEGF-D might be involved in the pathogenesis of nAMD or PCV. None of the 34 cytokines may help to differentiate nAMD and PCV.
Keywords
Aqueous humor, Cytokines, Age-related macular degeneration, Polypoidal choroidal vasculopathy
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CURRENT STATUS: Published
Version 3
Posted 19 Dec, 2019
Published
On 08 Jan, 2020
No community comments so far
Submission checks complete
On 14 Dec, 2019
Editorial decision: Accept
On 13 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 10 Dec, 2019
Reviewer #2 agreed
On 27 Nov, 2019
Review #2 received
Received 27 Nov, 2019
Reviewer #1 agreed
On 25 Nov, 2019
Reviewers invited
Invitations sent on 24 Nov, 2019
Editor assigned
On 21 Nov, 2019
Submission checks complete
On 20 Nov, 2019
Editor invited
On 20 Nov, 2019
No comments provided
Review #2 received
Received 14 Nov, 2019
Editorial decision: Major revision
On 14 Nov, 2019
Reviewer #3 agreed
On 14 Nov, 2019
Review #3 received
Received 14 Nov, 2019
Reviewer #2 agreed
On 12 Oct, 2019
Review #1 received
Received 26 Aug, 2019
Reviewer #1 agreed
On 23 Aug, 2019
Reviewers invited
Invitations sent on 22 Aug, 2019
Submission checks complete
On 21 Aug, 2019
Editor assigned
On 21 Aug, 2019
First submitted
On 20 Aug, 2019
Editor invited
On 20 Aug, 2019
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Subject Areas
Ophthalmology
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This preprint is under consideration at BMC Ophthalmology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Comparison of Cytokine Levels in the Aqueous Humor of Polypoidal Choroidal Vasculopathy and Neovascular Age-Related Macular Degeneration Patients
Youxin Chen
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7231-5058
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 19 Dec, 2019
Published
On 08 Jan, 2020
No community comments so far
Submission checks complete
On 14 Dec, 2019
Editorial decision: Accept
On 13 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 10 Dec, 2019
Reviewer #2 agreed
On 27 Nov, 2019
Review #2 received
Received 27 Nov, 2019
Reviewer #1 agreed
On 25 Nov, 2019
Reviewers invited
Invitations sent on 24 Nov, 2019
Editor assigned
On 21 Nov, 2019
Submission checks complete
On 20 Nov, 2019
Editor invited
On 20 Nov, 2019
No comments provided
Review #2 received
Received 14 Nov, 2019
Editorial decision: Major revision
On 14 Nov, 2019
Reviewer #3 agreed
On 14 Nov, 2019
Review #3 received
Received 14 Nov, 2019
Reviewer #2 agreed
On 12 Oct, 2019
Review #1 received
Received 26 Aug, 2019
Reviewer #1 agreed
On 23 Aug, 2019
Reviewers invited
Invitations sent on 22 Aug, 2019
Submission checks complete
On 21 Aug, 2019
Editor assigned
On 21 Aug, 2019
First submitted
On 20 Aug, 2019
Editor invited
On 20 Aug, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Ophthalmology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Ophthalmology.
Background
The concentrations of cytokines in the aqueous humor from neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV) may vary. The study was conducted to compare various cytokine levels in the aqueous humor of eyes with PCV, nAMD and control.
Methods
The present case control study included 49 treatment-naïve eyes from 49 patients (PCV 24, nAMD 11, and cataract 14 eyes). Totally 34 angiogenic and inflammatory cytokines in the aqueous humor were measured by Luminex bead-based multiplex array.
Results
After adjusting for gender and age by multivariate logistic analysis, concentrations of IL-31, LIF, SDF1-α, VEGF-A, VEGF-D were significantly higher in eyes with nAMD or PCV compared with control eyes (all P<0.05, times in nAMD: 59.5, 6.0, 7.0, 4.5, 5.6, respectively, times in PCV: 51.9, 5.21, 6.6, 4.0, 5.1, respectively ), and concentrations of HGF, IP-10, MCP-1, IL-13 were significantly lower in eyes with nAMD or PCV than in control eyes (all P<0.05, times in nAMD: 2.6, 2.0, 4.5, 4.7, respectively, times in PCV: 1.9, 3.0, 3.0, 2.8, respectively), but none of the 34 cytokines, including VEGF and IL-8, showed significantly different between eyes with nAMD and PCV.
Conclusions
Various cytokines involved in inflammation and angiogenesis including elevated IL-31, LIF, SDF1-α, VEGF-A, VEGF-D might be involved in the pathogenesis of nAMD or PCV. None of the 34 cytokines may help to differentiate nAMD and PCV.