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Research article
Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing
Dongwei Huang
Anhui Academy of Agricultural Sciences
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive.
Results: In this study, hair follicle density at different body sites of Wan strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR).
Conclusions: LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.
Keywords
Skin, Hair follicle density, Wool production, Histological analysis, LncRNA expression, RNA sequencing, Angora rabbit
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This is a list of supplementary files associated with this preprint. Click to download.
- AuthorChecklist.pdf
- checklists.docx
- SupplementaryData2.xlsx
- SupplementaryData1.xlsx
- Additionalfile1.pdf
Additional file 1: Table S1. The analyses of reads mapped to the Rabbit reference genome.
- Additionalfile2.pdf
Additional file 2: Figure S1. Expression level analysis of the lncRNAs and protein-coding genes.
- Additionalfile3.pdf
Additional file 3: Table S2. The wool production of the rabbits. “L” and “H” represent low wool production and high wool production groups, respectively.
- Additionalfile4.pdf
Additional file 4: Table S3. Primers for q-PCR. F1, forward primer. R2, reverse primer.
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On 04 Jan, 2021
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On 31 Dec, 2020
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On 31 Dec, 2020
Editor invited
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No comments provided
Editorial decision: Minor revision
On 13 Dec, 2020
Review #2 received
Received 19 Oct, 2020
Review #1 received
Received 18 Oct, 2020
Reviewer #3 agreed
On 08 Oct, 2020
Reviewer #2 agreed
On 27 Sep, 2020
Reviewer #1 agreed
On 24 Sep, 2020
Reviewers invited
Invitations sent on 23 Sep, 2020
Editor assigned
On 10 Aug, 2020
Submission checks complete
On 09 Aug, 2020
Editor invited
On 09 Aug, 2020
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing
Dongwei Huang
Anhui Academy of Agricultural Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 13 Jan, 2021
No community comments so far
Reviewer #2 agreed
On 07 Jan, 2021
Review #2 received
Received 07 Jan, 2021
Review #1 received
Received 06 Jan, 2021
Reviewers invited
Invitations sent on 04 Jan, 2021
Reviewer #1 agreed
On 04 Jan, 2021
Editor assigned
On 31 Dec, 2020
Submission checks complete
On 31 Dec, 2020
Editor invited
On 31 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 13 Dec, 2020
Review #2 received
Received 19 Oct, 2020
Review #1 received
Received 18 Oct, 2020
Reviewer #3 agreed
On 08 Oct, 2020
Reviewer #2 agreed
On 27 Sep, 2020
Reviewer #1 agreed
On 24 Sep, 2020
Reviewers invited
Invitations sent on 23 Sep, 2020
Editor assigned
On 10 Aug, 2020
Submission checks complete
On 09 Aug, 2020
Editor invited
On 09 Aug, 2020
Version (no preprint)
Posted 15 Jul, 2020
This version was not preprinted
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive.
Results: In this study, hair follicle density at different body sites of Wan strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR).
Conclusions: LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7