Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing
Background: Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive.
Results: In this study, hair follicle density at different body sites of Wan strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR).
Conclusions: LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
Additional file 1: Table S1. The analyses of reads mapped to the Rabbit reference genome.
Additional file 2: Figure S1. Expression level analysis of the lncRNAs and protein-coding genes.
Additional file 3: Table S2. The wool production of the rabbits. “L” and “H” represent low wool production and high wool production groups, respectively.
Additional file 4: Table S3. Primers for q-PCR. F1, forward primer. R2, reverse primer.
Posted 13 Jan, 2021
On 07 Jan, 2021
Received 07 Jan, 2021
Received 06 Jan, 2021
Invitations sent on 04 Jan, 2021
On 04 Jan, 2021
On 31 Dec, 2020
On 31 Dec, 2020
On 31 Dec, 2020
On 13 Dec, 2020
Received 19 Oct, 2020
Received 18 Oct, 2020
On 08 Oct, 2020
On 27 Sep, 2020
On 24 Sep, 2020
Invitations sent on 23 Sep, 2020
On 10 Aug, 2020
On 09 Aug, 2020
On 09 Aug, 2020
Posted 15 Jul, 2020
Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing
Posted 13 Jan, 2021
On 07 Jan, 2021
Received 07 Jan, 2021
Received 06 Jan, 2021
Invitations sent on 04 Jan, 2021
On 04 Jan, 2021
On 31 Dec, 2020
On 31 Dec, 2020
On 31 Dec, 2020
On 13 Dec, 2020
Received 19 Oct, 2020
Received 18 Oct, 2020
On 08 Oct, 2020
On 27 Sep, 2020
On 24 Sep, 2020
Invitations sent on 23 Sep, 2020
On 10 Aug, 2020
On 09 Aug, 2020
On 09 Aug, 2020
Posted 15 Jul, 2020
Background: Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive.
Results: In this study, hair follicle density at different body sites of Wan strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR).
Conclusions: LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7