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Research article
Nutrient-imbalanced conditions shift the interplay between zooplankton and gut microbiota
Hongbin Liu
Hong Kong University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3184-2898
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background
Nutrient stoichiometry of phytoplankton frequently changes with aquatic ambient nutrient concentrations, which is mainly influenced by anthropogenic water treatment and the ecosystem dynamics. Consequently, the stoichiometry of phytoplankton can markedly alter the metabolism and growth of zooplankton. However, the effects of nutrient-imbalanced prey on the interplay between zooplankton and their gut microbiota remain unknown. Using metatranscriptome, a 16s rRNA amplicon-based neutral community model (NCM) and experimental validation, we investigated the interactions between Daphnia magna and its gut microbiota in a nutrient-imbalanced algal diet.
Results
Our results showed that in nutrient-depleted water, the nutrient-enriched zooplankton gut stimulated the accumulation of microbial polyphosphate in fecal pellets under phosphorus limitation and the microbial assimilation of ammonia under nitrogen limitation. Compared with the nutrient replete group, both N and P limitation markedly promoted the gene expression of the gut microbiome for organic matter degradation but repressed that for anaerobic metabolisms. In the nutrient limited diet, the gut microbial community exhibited a higher fit to NCM (R2=0.624 and 0.781, for N- and P-limitation, respectively) when compared with the Control group (R2=0.542), suggesting increased ambient-gut exchange process favored by compensatory feeding. Further, an additional axenic grazing experiment revealed that the growth of D. magna can still benefit from gut microbiota under a nutrient-imbalanced diet.
Conclusions
Together, these results demonstrated that under a nutrient-imbalanced diet, the microbes not only benefit themselves by absorbing excess nutrients inside the zooplankton gut but also help zooplankton to survive during nutrient limitation.
Keywords
Metatranscriptomic analysis, Daphnia magna, Gut microbe, Elemental stoichiometry, Stoichiometric homeostasis
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On 05 Nov, 2020
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On 03 Nov, 2020
Reviewer #1 agreed
On 03 Nov, 2020
Editor assigned
On 02 Nov, 2020
Reviewers invited
Invitations sent on 02 Nov, 2020
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On 02 Nov, 2020
Editor invited
On 02 Nov, 2020
No comments provided
Editorial decision: Major revision
On 02 Oct, 2020
Review #3 received
Received 08 Sep, 2020
Review #2 received
Received 06 Sep, 2020
Review #1 received
Received 18 Aug, 2020
Reviewer #3 agreed
On 16 Aug, 2020
Reviewer #2 agreed
On 14 Aug, 2020
Reviewer #1 agreed
On 03 Aug, 2020
Reviewers invited
Invitations sent on 30 Jul, 2020
Editor assigned
On 15 Jul, 2020
First submitted
On 14 Jul, 2020
Submission checks complete
On 14 Jul, 2020
Editor invited
On 14 Jul, 2020
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Epigenetics & Genomics
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Nutrient-imbalanced conditions shift the interplay between zooplankton and gut microbiota
Hongbin Liu
Hong Kong University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3184-2898
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 22 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editorial decision: Minor revision
On 07 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 01 Dec, 2020
Review #2 received
Received 24 Nov, 2020
Review #1 received
Received 23 Nov, 2020
Review #3 received
Received 07 Nov, 2020
Reviewer #3 agreed
On 05 Nov, 2020
Reviewer #2 agreed
On 03 Nov, 2020
Reviewer #1 agreed
On 03 Nov, 2020
Editor assigned
On 02 Nov, 2020
Reviewers invited
Invitations sent on 02 Nov, 2020
Submission checks complete
On 02 Nov, 2020
Editor invited
On 02 Nov, 2020
No comments provided
Editorial decision: Major revision
On 02 Oct, 2020
Review #3 received
Received 08 Sep, 2020
Review #2 received
Received 06 Sep, 2020
Review #1 received
Received 18 Aug, 2020
Reviewer #3 agreed
On 16 Aug, 2020
Reviewer #2 agreed
On 14 Aug, 2020
Reviewer #1 agreed
On 03 Aug, 2020
Reviewers invited
Invitations sent on 30 Jul, 2020
Editor assigned
On 15 Jul, 2020
First submitted
On 14 Jul, 2020
Submission checks complete
On 14 Jul, 2020
Editor invited
On 14 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background
Nutrient stoichiometry of phytoplankton frequently changes with aquatic ambient nutrient concentrations, which is mainly influenced by anthropogenic water treatment and the ecosystem dynamics. Consequently, the stoichiometry of phytoplankton can markedly alter the metabolism and growth of zooplankton. However, the effects of nutrient-imbalanced prey on the interplay between zooplankton and their gut microbiota remain unknown. Using metatranscriptome, a 16s rRNA amplicon-based neutral community model (NCM) and experimental validation, we investigated the interactions between Daphnia magna and its gut microbiota in a nutrient-imbalanced algal diet.
Results
Our results showed that in nutrient-depleted water, the nutrient-enriched zooplankton gut stimulated the accumulation of microbial polyphosphate in fecal pellets under phosphorus limitation and the microbial assimilation of ammonia under nitrogen limitation. Compared with the nutrient replete group, both N and P limitation markedly promoted the gene expression of the gut microbiome for organic matter degradation but repressed that for anaerobic metabolisms. In the nutrient limited diet, the gut microbial community exhibited a higher fit to NCM (R2=0.624 and 0.781, for N- and P-limitation, respectively) when compared with the Control group (R2=0.542), suggesting increased ambient-gut exchange process favored by compensatory feeding. Further, an additional axenic grazing experiment revealed that the growth of D. magna can still benefit from gut microbiota under a nutrient-imbalanced diet.
Conclusions
Together, these results demonstrated that under a nutrient-imbalanced diet, the microbes not only benefit themselves by absorbing excess nutrients inside the zooplankton gut but also help zooplankton to survive during nutrient limitation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8