A transdermal drug delivery system capable of transporting the active substance through skin is alternative path for drug delivery for different purposes. In an attempt to demonstrate the permeability of the active substance through the skin layers of the rats, the amphiphilic MTC-Y carrier was combined with fluorochromes of different chemical properties. After extraction, the skin material was subjected histological examination under fluorescence microscope Nikon Eclipse 80i UV-2A filter (EX330-380, DM-400, BA-420). Moreover, histological slides routinely stained with haematoxylin were analysed. Results indicate that use of the MTC-Y carrier seems to be very promising compound for drug delivery both locally and systematically