Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture
Background: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth.
Results: In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7,373 in the leaves. In seedlings grown in the presence of AC, 9,460 DEGs were upregulated and 7,483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, including PLA, CYP73A, COMT, CYP84A, and 4CL , the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, including EIN3, BZR1, JAR1, JAZ , and PR1 , and downregulated genes related to plant growth inhibition, including BKI1, ARR-B , DELLA , and ABF .
Conclusions: Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance.
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Posted 10 Jun, 2020
On 06 Jul, 2020
On 23 Jun, 2020
On 04 Jun, 2020
On 03 Jun, 2020
On 03 Jun, 2020
On 04 Apr, 2020
Received 18 Mar, 2020
Received 15 Mar, 2020
On 08 Mar, 2020
On 05 Mar, 2020
Received 07 Dec, 2019
On 24 Nov, 2019
Invitations sent on 21 Nov, 2019
On 27 Aug, 2019
On 27 Aug, 2019
On 23 Aug, 2019
On 18 Aug, 2019
Transcriptome analysis of activated charcoal-induced growth promotion of wheat seedlings in tissue culture
Posted 10 Jun, 2020
On 06 Jul, 2020
On 23 Jun, 2020
On 04 Jun, 2020
On 03 Jun, 2020
On 03 Jun, 2020
On 04 Apr, 2020
Received 18 Mar, 2020
Received 15 Mar, 2020
On 08 Mar, 2020
On 05 Mar, 2020
Received 07 Dec, 2019
On 24 Nov, 2019
Invitations sent on 21 Nov, 2019
On 27 Aug, 2019
On 27 Aug, 2019
On 23 Aug, 2019
On 18 Aug, 2019
Background: Activated charcoal (AC) is highly adsorbent and is often used to promote seedling growth in plant tissue culture; however, the underlying molecular mechanism remains unclear. In this study, root and leaf tissues of 10-day-old seedlings grown via immature embryo culture in the presence or absence of AC in the culture medium were subjected to global transcriptome analysis by RNA sequencing to provide insights into the effects of AC on seedling growth.
Results: In total, we identified 18,555 differentially expressed genes (DEGs). Of these, 11,182 were detected in the roots and 7,373 in the leaves. In seedlings grown in the presence of AC, 9,460 DEGs were upregulated and 7,483 DEGs were downregulated in the presence of AC as compared to the control. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed 254 DEG-enriched pathways, 226 of which were common between roots and leaves. Further analysis of the major metabolic pathways revealed that AC stimulated the expression of nine genes in the phenylpropanoid biosynthesis pathway, including PLA, CYP73A, COMT, CYP84A, and 4CL , the protein products of which promote cell differentiation and seedling growth. Further, AC upregulated genes involved in plant hormone signaling related to stress resistance and disease resistance, including EIN3, BZR1, JAR1, JAZ , and PR1 , and downregulated genes related to plant growth inhibition, including BKI1, ARR-B , DELLA , and ABF .
Conclusions: Growth medium containing AC promotes seedling growth by increasing the expression of certain genes in the phenylpropanoid biosynthesis pathway, which are related to cell differentiation and seedling growth, as well as genes involved in plant hormone signaling, which is related to resistance.
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