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Original article
Auxin, abscisic acid and jasmonate are the central players in rice sheath rot caused by Sarocladium oryzae and Pseudomonas fuscovaginae
Monica Hofte
Ghent University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0850-3249
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Rice.
Sheath rot is an emerging rice disease that causes severe yield losses worldwide. The main causal agents are the toxin producers Sarocladium oryzae and Pseudomonas fuscovaginae. The fungus S. oryzae produces helvolic acid and cerulenin and the bacterium P. fuscovaginae produces cyclic lipopeptides. Helvolic acid and the lipopeptide, fuscopeptin, inhibit membrane-bound H+-ATPase pumps in the rice plant. To manage rice sheath rot, a better understanding of the host response and virulence strategies of the pathogens is required. This study investigated the interaction of the sheath rot pathogens with their host and the role of their toxins herein. Japonica rice was inoculated with high- and low-helvolic acid-producing S. oryzae isolates or with P. fuscovaginae wild type and fuscopeptin mutant strains. During infection, cerulenin, helvolic acid and the phytohormones abscisic acid, jasmonate, auxin and salicylic acid were quantified in the sheath. In addition, disease severity and grain yield parameters were assessed. Rice plants responded to high-toxin-producing S. oryzae and P. fuscovaginae strains with an increase in abscisic acid, jasmonate and auxin levels. We conclude that, for both pathogens, toxins play a core role during sheath rot infection. S. oryzae and P. fuscovaginae interact with their host in a similar way. This may explain why both sheath rot pathogens cause very similar symptoms despite their different nature.
Keywords
abscisic acid, auxin, H+-ATPase, jasmonate, Pseudomonas fuscovaginae, rice, Sarocladium oryzae, sheath rot, helvolic acid, fuscopeptin
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CURRENT STATUS: Published
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Posted 23 Sep, 2020
Published
On 26 Nov, 2020
No community comments so far
Review #1 received
Received 03 Nov, 2020
Editorial decision: Accept
On 03 Nov, 2020
Review #2 received
Received 30 Oct, 2020
Reviewer #2 agreed
On 24 Oct, 2020
Reviewers invited
Invitations sent on 23 Oct, 2020
Reviewer #1 agreed
On 23 Oct, 2020
Editor assigned
On 21 Sep, 2020
Submission checks complete
On 20 Sep, 2020
Editor invited
On 20 Sep, 2020
No comments provided
Review #2 received
Received 27 Aug, 2020
Editorial decision: Major revision
On 27 Aug, 2020
Reviewer #2 agreed
On 13 Aug, 2020
Review #1 received
Received 12 Aug, 2020
Reviewers invited
Invitations sent on 28 Jul, 2020
Reviewer #1 agreed
On 28 Jul, 2020
Editor assigned
On 20 Jul, 2020
Submission checks complete
On 19 Jul, 2020
Editor invited
On 19 Jul, 2020
First submitted
On 17 Jul, 2020
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This preprint is under consideration at Rice. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Auxin, abscisic acid and jasmonate are the central players in rice sheath rot caused by Sarocladium oryzae and Pseudomonas fuscovaginae
Monica Hofte
Ghent University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0850-3249
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 23 Sep, 2020
Published
On 26 Nov, 2020
No community comments so far
Review #1 received
Received 03 Nov, 2020
Editorial decision: Accept
On 03 Nov, 2020
Review #2 received
Received 30 Oct, 2020
Reviewer #2 agreed
On 24 Oct, 2020
Reviewers invited
Invitations sent on 23 Oct, 2020
Reviewer #1 agreed
On 23 Oct, 2020
Editor assigned
On 21 Sep, 2020
Submission checks complete
On 20 Sep, 2020
Editor invited
On 20 Sep, 2020
No comments provided
Review #2 received
Received 27 Aug, 2020
Editorial decision: Major revision
On 27 Aug, 2020
Reviewer #2 agreed
On 13 Aug, 2020
Review #1 received
Received 12 Aug, 2020
Reviewers invited
Invitations sent on 28 Jul, 2020
Reviewer #1 agreed
On 28 Jul, 2020
Editor assigned
On 20 Jul, 2020
Submission checks complete
On 19 Jul, 2020
Editor invited
On 19 Jul, 2020
First submitted
On 17 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Rice.
Sheath rot is an emerging rice disease that causes severe yield losses worldwide. The main causal agents are the toxin producers Sarocladium oryzae and Pseudomonas fuscovaginae. The fungus S. oryzae produces helvolic acid and cerulenin and the bacterium P. fuscovaginae produces cyclic lipopeptides. Helvolic acid and the lipopeptide, fuscopeptin, inhibit membrane-bound H+-ATPase pumps in the rice plant. To manage rice sheath rot, a better understanding of the host response and virulence strategies of the pathogens is required. This study investigated the interaction of the sheath rot pathogens with their host and the role of their toxins herein. Japonica rice was inoculated with high- and low-helvolic acid-producing S. oryzae isolates or with P. fuscovaginae wild type and fuscopeptin mutant strains. During infection, cerulenin, helvolic acid and the phytohormones abscisic acid, jasmonate, auxin and salicylic acid were quantified in the sheath. In addition, disease severity and grain yield parameters were assessed. Rice plants responded to high-toxin-producing S. oryzae and P. fuscovaginae strains with an increase in abscisic acid, jasmonate and auxin levels. We conclude that, for both pathogens, toxins play a core role during sheath rot infection. S. oryzae and P. fuscovaginae interact with their host in a similar way. This may explain why both sheath rot pathogens cause very similar symptoms despite their different nature.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7