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Research article
Platelet Proteome Changes in Dogs with Chronic Heart Failure
Zeki YILMAZ
Dep. of Internal Medicine, Uludag University Faculty of Veterinary Medicine, Bursa
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9836-0749
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in chronic heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n=10) and dogs with CHF due to myxomatous mitral valve degeneration (n=10) were used. Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris.
Results: Totally 107 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in the CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in the CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses.
Conclusion: This study describes by first time the changes in the protein composition in platelets of dogs with CHF. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF and could be a tool for further investigations about the prevention and treatment of this disease.
Keywords
Platelet proteomic, heart failure, myxomatous mitral valve disease, dogs
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CURRENT STATUS: Published
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On 07 Nov, 2020
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On 07 Nov, 2020
Editor invited
On 07 Nov, 2020
No comments provided
Review #2 received
Received 24 Oct, 2020
Editorial decision: Minor revision
On 24 Oct, 2020
Review #1 received
Received 21 Oct, 2020
Reviewer #2 agreed
On 18 Oct, 2020
Reviewer #1 agreed
On 12 Oct, 2020
Reviewers invited
Invitations sent on 11 Oct, 2020
Editor assigned
On 05 Oct, 2020
Submission checks complete
On 04 Oct, 2020
Editor invited
On 04 Oct, 2020
Version 1
Posted 04 Aug, 2020
No comments provided
Editorial decision: Major revision
On 21 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Reviewer #2 agreed
On 31 Aug, 2020
Review #1 received
Received 25 Aug, 2020
Reviewer #1 agreed
On 02 Aug, 2020
Reviewers invited
Invitations sent on 31 Jul, 2020
Editor assigned
On 29 Jul, 2020
Submission checks complete
On 28 Jul, 2020
Editor invited
On 28 Jul, 2020
First submitted
On 23 Jul, 2020
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Platelet Proteome Changes in Dogs with Chronic Heart Failure
Zeki YILMAZ
Dep. of Internal Medicine, Uludag University Faculty of Veterinary Medicine, Bursa
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9836-0749
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
No comments provided
Editor assigned
On 07 Nov, 2020
Submission checks complete
On 07 Nov, 2020
Editor invited
On 07 Nov, 2020
No comments provided
Review #2 received
Received 24 Oct, 2020
Editorial decision: Minor revision
On 24 Oct, 2020
Review #1 received
Received 21 Oct, 2020
Reviewer #2 agreed
On 18 Oct, 2020
Reviewer #1 agreed
On 12 Oct, 2020
Reviewers invited
Invitations sent on 11 Oct, 2020
Editor assigned
On 05 Oct, 2020
Submission checks complete
On 04 Oct, 2020
Editor invited
On 04 Oct, 2020
Version 1
Posted 04 Aug, 2020
No comments provided
Editorial decision: Major revision
On 21 Sep, 2020
Review #2 received
Received 18 Sep, 2020
Reviewer #2 agreed
On 31 Aug, 2020
Review #1 received
Received 25 Aug, 2020
Reviewer #1 agreed
On 02 Aug, 2020
Reviewers invited
Invitations sent on 31 Jul, 2020
Editor assigned
On 29 Jul, 2020
Submission checks complete
On 28 Jul, 2020
Editor invited
On 28 Jul, 2020
First submitted
On 23 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in chronic heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n=10) and dogs with CHF due to myxomatous mitral valve degeneration (n=10) were used. Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris.
Results: Totally 107 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in the CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in the CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses.
Conclusion: This study describes by first time the changes in the protein composition in platelets of dogs with CHF. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF and could be a tool for further investigations about the prevention and treatment of this disease.