Platelet Proteome Changes in Dogs with Chronic Heart Failure
Background: Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in chronic heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n=10) and dogs with CHF due to myxomatous mitral valve degeneration (n=10) were used. Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris.
Results: Totally 107 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in the CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in the CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses.
Conclusion: This study describes by first time the changes in the protein composition in platelets of dogs with CHF. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF and could be a tool for further investigations about the prevention and treatment of this disease.
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Platelet Proteome Changes in Dogs with Chronic Heart Failure
On 07 Nov, 2020
On 07 Nov, 2020
On 07 Nov, 2020
Received 24 Oct, 2020
On 24 Oct, 2020
Received 21 Oct, 2020
On 18 Oct, 2020
On 12 Oct, 2020
Invitations sent on 11 Oct, 2020
On 05 Oct, 2020
On 04 Oct, 2020
On 04 Oct, 2020
Posted 04 Aug, 2020
On 21 Sep, 2020
Received 18 Sep, 2020
On 31 Aug, 2020
Received 25 Aug, 2020
On 02 Aug, 2020
Invitations sent on 31 Jul, 2020
On 29 Jul, 2020
On 28 Jul, 2020
On 28 Jul, 2020
On 23 Jul, 2020
Background: Platelets play a central role in the development of cardiovascular diseases and changes in their proteins are involved in the pathophysiology of heart diseases in humans. There is lack of knowledge about the possible role of platelets in chronic heart failure (CHF) in dogs. Thus, this study aimed to investigate the changes in global platelet proteomes in dogs with CHF, to clarify the possible role of platelets in the physiopathology of this disease. Healthy-dogs (n=10) and dogs with CHF due to myxomatous mitral valve degeneration (n=10) were used. Blood samples were collected into tubes with acid-citrate-dextrose, and platelet-pellets were obtained by centrifuge and washing steps. Platelet-proteomes were identified using LC-MS based label-free differential proteome expression analysis method and matched according to protein database for Canis lupus familiaris.
Results: Totally 107 different proteins were identified in the platelets of the dogs being 4 out of them were significantly up-regulated and 6 down-regulated in the CHF dogs. Guanine-nucleotide-binding protein, apolipoproteins (A-II and C-III) and clusterin levels increased, but CXC-motif-chemokine-10, cytochrome-C-oxidase-subunit-2, cathepsin-D, serine/threonine-protein-phosphatase-PP1-gamma-catalytic-subunit, creatine-kinase-B-type and myotrophin levels decreased in the CHF dogs. These proteins are associated with several molecular functions, biological processes, signaling systems and immune-inflammatory responses.
Conclusion: This study describes by first time the changes in the protein composition in platelets of dogs with CHF. Our findings provide a resource for increase the knowledge about the proteome of canine platelets and their roles in CHF and could be a tool for further investigations about the prevention and treatment of this disease.