Transcriptomic analysis reveals hub genes and subnetworks related to ROS metabolism in Hylocereus undatus through novel superoxide scavenger trypsin treatment during storage
Background: It was demonstrated in our previous research that trypsin scavenges superoxide anions. In this study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus.
Results: Trypsin significantly delayed the weight loss and decreased the levels of ROS and membrane lipid peroxidation. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of ROS genes that were up- or downregulated following trypsin treatment by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The current results showed that through the regulation of the expression of hub redox enzymes, especially thioredoxin-related proteins, trypsin can maintain low levels of endogenous active oxygen species, reduce malondialdehyde content and delay fruit aging. In addition, the results of protein-protein interaction networks suggested that the downregulated NAD(P)H and lignin pathways might be the key regulatory mechanisms governed by trypsin.
Conclusions: Trypsin significantly prolonged the storage life of H. undatus through regulatory on the endogenous ROS metabolism. As a new biopreservative, trypsin is highly efficient, safe and economical. Therefore, trypsin possesses technical feasibility for the quality control of fruit storage.
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Posted 19 Jun, 2020
On 16 Jun, 2020
Received 12 Jun, 2020
Received 09 Jun, 2020
On 02 Jun, 2020
On 01 Jun, 2020
Invitations sent on 01 Jun, 2020
On 01 Jun, 2020
On 31 May, 2020
On 31 May, 2020
On 26 May, 2020
Received 12 May, 2020
On 01 May, 2020
Received 14 Feb, 2020
Invitations sent on 01 Oct, 2019
On 01 Oct, 2019
On 02 Sep, 2019
On 02 Sep, 2019
On 27 Aug, 2019
On 24 Aug, 2019
Transcriptomic analysis reveals hub genes and subnetworks related to ROS metabolism in Hylocereus undatus through novel superoxide scavenger trypsin treatment during storage
Posted 19 Jun, 2020
On 16 Jun, 2020
Received 12 Jun, 2020
Received 09 Jun, 2020
On 02 Jun, 2020
On 01 Jun, 2020
Invitations sent on 01 Jun, 2020
On 01 Jun, 2020
On 31 May, 2020
On 31 May, 2020
On 26 May, 2020
Received 12 May, 2020
On 01 May, 2020
Received 14 Feb, 2020
Invitations sent on 01 Oct, 2019
On 01 Oct, 2019
On 02 Sep, 2019
On 02 Sep, 2019
On 27 Aug, 2019
On 24 Aug, 2019
Background: It was demonstrated in our previous research that trypsin scavenges superoxide anions. In this study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus.
Results: Trypsin significantly delayed the weight loss and decreased the levels of ROS and membrane lipid peroxidation. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of ROS genes that were up- or downregulated following trypsin treatment by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The current results showed that through the regulation of the expression of hub redox enzymes, especially thioredoxin-related proteins, trypsin can maintain low levels of endogenous active oxygen species, reduce malondialdehyde content and delay fruit aging. In addition, the results of protein-protein interaction networks suggested that the downregulated NAD(P)H and lignin pathways might be the key regulatory mechanisms governed by trypsin.
Conclusions: Trypsin significantly prolonged the storage life of H. undatus through regulatory on the endogenous ROS metabolism. As a new biopreservative, trypsin is highly efficient, safe and economical. Therefore, trypsin possesses technical feasibility for the quality control of fruit storage.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6