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Research article
HUI ZHANG
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, China
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9904-3833
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Staphylococcus aureus (S. aureus) is the leading cause of various infective diseases including topical soft tissue infections. Due to an increasing prevalence of the methicillin-resistant Staphylococcus aureus (MRSA), S. aureus infection become more challenging to being treated over recent years. The goals of this study were to investigate the roles of antisense yycF/G RNAs in the regulation of the biofilm formation and pathogenicity. Methods: MRSA ASyycF/G overexpression mutants were constructed. The biofilm biomass was determined by crystal violet microtiter assay and scanning electron mi-ceroscopy (SEM). Quantitative RT-PCR and western blotting analysis were used to detect the transcripts and translations of biofilm-related genes. The effects of the antisense RNAs (ASyycF/G) strategy on the susceptibility of biofilm-producing S. aureus to hydrogen peroxide were also investigated. Result: The ASyycF/G transcript leaded to reduction in the biofilm formation. Overexpression of ASyycF/G inhibited the transcripts and translations of biofilm-related genes. The sensibility to vancomycin was improved in ASyycF/G overexpression MRSA. Conclusions: The biofilm biomass and the transcripts of the pathogenicity associated genes decreased in the ASyycF/G overexpression mutant. Thus, the current evidence may provide a supplementary strategy for managing MRSA infections.
Keywords
Antisense, Biofilm formation, Staphylococcus aureus, YycFG
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
HUI ZHANG
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, China
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9904-3833
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 May, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Staphylococcus aureus (S. aureus) is the leading cause of various infective diseases including topical soft tissue infections. Due to an increasing prevalence of the methicillin-resistant Staphylococcus aureus (MRSA), S. aureus infection become more challenging to being treated over recent years. The goals of this study were to investigate the roles of antisense yycF/G RNAs in the regulation of the biofilm formation and pathogenicity. Methods: MRSA ASyycF/G overexpression mutants were constructed. The biofilm biomass was determined by crystal violet microtiter assay and scanning electron mi-ceroscopy (SEM). Quantitative RT-PCR and western blotting analysis were used to detect the transcripts and translations of biofilm-related genes. The effects of the antisense RNAs (ASyycF/G) strategy on the susceptibility of biofilm-producing S. aureus to hydrogen peroxide were also investigated. Result: The ASyycF/G transcript leaded to reduction in the biofilm formation. Overexpression of ASyycF/G inhibited the transcripts and translations of biofilm-related genes. The sensibility to vancomycin was improved in ASyycF/G overexpression MRSA. Conclusions: The biofilm biomass and the transcripts of the pathogenicity associated genes decreased in the ASyycF/G overexpression mutant. Thus, the current evidence may provide a supplementary strategy for managing MRSA infections.
Figure 1
Figure 2
Figure 3
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