TAC on stool vs. benchmark
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Pathogen
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This study – total number of stool samples tested by TAC = 109
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Reported literature
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Median storage duration (IQR) and no. of samples:
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PPA and NPA
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Study Design
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PPA and NPA
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ETEC
LT (labile toxin)
ST (stable toxin)
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No of samples: 59
11.5 (7.2-12.0) years
≤ 3 years: 13 samples
5-13 years: 46 samples
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PPA:62.7 (49.2-75.0)
NPA:70.0 (55.4-82.1)
Stratified by duration:
≤ 3 years: PPA: 69.2(38.6-90.9)
NPA: 66.7(47.2-82.7)
5-13 years: PPA: 60.8(45.4-74.9)
NPA: 75.0(50.9-91.3)
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Sample size: 109 (10 ETEC positive)
Comparator: Culture + PCR of 5 colonies
PCR platform: TAC
Storage duration: 2-4 years (10)
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PPA:100.0 (69.2-100.0)
NPA (ETEC LT): 55.0 (41.6-67.9)
NPA (ETEC ST): 69.7 (57.1-80.4)
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Campylobacter
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No of samples:21
2.6 (2.6-11.4) years
≤ 3 years: 11
5-13 years: 10
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PPA:66.6(43.0-85.4)
NPA:69.3(58.6-78.7)
Stratified by duration:
≤ 3 years: PPA: 63.6(30.8-89.0)
NPA: 68.8(50.0-83.8)
5-13 years: PPA: 70.0(34.8-93.3)
NPA: 69.6(56.0-81.2)
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Sample size: 1557 (35 Campylobacter positive)
Comparator: Culture
PCR platform: BioFire FilmArray GI panel
Storage duration: days-weeks (4)
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PPA:97.1 (85.1-99.9)
NPA:98.4 (97.7-99.0)
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Shigella/EIEC
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No of samples:10
7.6 (6.7-10) years
5-13 years: 10
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PPA:100.0(69.2-100.0)
NPA:82.8(74.0-89.6)
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Sample size: 1557 (49 Shigella/EIEC positive)
Comparator: Culture (PCR for EIEC)
PCR platform: BioFire FilmArray GI panel
Storage duration: days-weeks (4)
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PPA:95.9 (86.0-99.5)
NPA:98.5 (99.5-130.0)
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Norovirus
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No of samples:19
2.6 (2.6-2.6) years
≤ 3 years: 19
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PPA:36.8(16.2-61.6)
NPA:80.0(70.2-87.6)
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Sample size: 1557 (55 Norovirus positive)
Comparator: RT-PCR
PCR platform: BioFire FilmArray GI panel
Storage duration: days-weeks (4)
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PPA:94.5 (84.9-98.9)
NPA:98.8 (98.1-99.3)
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TAC on FTA card vs. standard microbiology (benchmark) This study – total number of FTA cards tested by TAC =261
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Pathogen
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Median storage duration (IQR) and no. of samples:
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PPA and NPA
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Stratified by duration:
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ETEC
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No of samples:70; 10.5(6.9-12.0) years
≤ 3 years: 14
5-13 years: 56
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PPA:55.7 (43.3-67.6)
NPA:85.3 (80.0-90.0)
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≤ 3 years: PPA: 57.1(28.8-82.3)
NPA: 86.0(75.0-93.3)
5-13 years: PPA: 55.4(41.4-68.6)
NPA: 85.0(77.6-90.8)
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Campylobacter
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No of samples: 59; 10.4(2.6-11.8) years
≤ 3 years: 28
5-13 years: 31
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PPA:62.7 (49.2-75.0)
NPA:82.2 (76.2-87.2)
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≤ 3 years: PPA:71.4(51.3-86.8)
NPA: 84.0(70.8-92.8)
5-13 years: PPA: 54.8(36.0-72.6)
NPA: 81.6(74.4-87.4)
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Shigella
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No of samples: 62; 6.0 (2.5-7.6) years
≤ 3 years: 6
5-13 years: 56
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PPA:88.7 (78.1-95.3)
NPA:86.4(81.0-91.0)
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≤ 3 years: PPA: 16.6(1.0-64.1)
NPA: 94.4(86.4-98.4)
5-13 years: PPA: 96.4(87.6-99.6)
NPA: 81.8(74.0-88.2)
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Norovirus
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No. of samples:27; 2.6 (2.6-2.6) years
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PPA:51.8(32.0-71.3)
NPA:97.8(95.0-99.3)
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N/A (all samples stored ≤ 3 years)
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TAC on FTA card vs. TAC on stool (benchmark)
|
Pathogen
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This study – total number of paired FTA cards and stool samples tested by TAC = 109
|
Reported Literature
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Median storage duration (IQR) and no. of samples:
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PPA and NPA
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Study Design
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PPA and NPA
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ETEC
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No. of paired samples:52;
median duration of FTA card storage: 8.8 (2.6-11.8) years
≤ 3 years: 19
5-13 years: 33
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PPA: 75.0(63.2-86.8)
NPA: 91.2(83.8-98.6)
Stratified by duration:
≤ 3 years: PPA: 73.6(48.8-90.8)
NPA: 91.6(73.0-98.9)
5-10 years: PPA: 75.8(57.7-88.9)
NPA: 90.9(75.6-98.0)
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Total sample size: 187
ETEC positive TAC on stool: 85
Storage duration: 2 years (9)
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PPA:90.6(82.2-96.0)
NPA:97.6(93.3-99.5)(3)
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Campylobacter
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No. of paired samples: 41; median storage duration: 7.0 (2.6-11.8) years
≤ 3 years: 17
5-10 years: 24
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PPA:58.5(42.1-73.6)
NPA:88.2(78.1-94.8)
Stratified by duration:
≤ 3 years: PPA: 47.0(22.9-72.2)
NPA: 92.3(74.8-99.0)
5-13 years: PPA: 66.6(44.6-84.4)
NPA: 85.7(71.4-94.6)
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Total sample size: 187
Campylobacter positive TAC on stool: 11
Storage duration: 2 years (9)
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PPA:90.9(58.7-99.8)
NPA:100.0(98.0-100.0)(3)
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Shigella
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Shigella positive TAC on stool: 27; median storage duration: 7.7(6.0-11.3) years
≤ 3 years: 5
5-10 years: 22
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PPA: 66.6(46.0-83.4)
NPA: 92.6(84.8-97.2)
Stratified by duration:
≤ 3 years: PPA: 40.0(5.2-85.3)
NPA: 94.7(82.2-99.4)
5-13 years: PPA: 72.7(49.8-89.2)
NPA: 90.9(78.3-97.4)
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Total sample size: 187
Shigella positive TAC on stool: 18
Storage duration: 2 years (9)
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PPA:88.9(65.2-98.6)
NPA:99.4(96.8-99.9)(3)
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Norovirus
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No of samples:19; median duration of FTA card storage: 2.6 (2.6-2.6) years
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PPA:28.0(12.0-49.4)
NPA:92.8(85.1-97.3)
Stratified by duration:
≤ 3 years: PPA: 36.4(10.9-69.2)
NPA: 81.2(636-92.8)
5-13 years: PPA: 21.4(4.6-50.8)
NPA: 100.0(93.2-100.0)
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Total sample size: 187
Norovirus positive TAC on stool: 24
Storage duration: 2 years (9)
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PPA:38.0(18.8-60.0)
NPA:100.0(98.9-100.0)(3)
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