Real-time data transmission is one of the objectives of MANET (mobile ad-hoc network) to handle emergencies like a forest fire, flood, and earthquake. In this scenario, quick delivery of data is itself a challenging task for MANET and there is a possibility of load imbalance due to packet transmission in between source-destination pairs via the shortest path due to congestion or control overhead. In this paper, a novel routing protocol, called ACOLBR (ACO based load balancing routing), is design to control the congestion and balancing the load among the multiple paths in between source to destination. The similarity between the environment of Ant Colony and the MANET inspires the authors to apply ACO (Ant colony optimization) technique during routing in MANET to control congestion and balance the load in the network. In our proposal, two colonies of ants (red/blue) carry their packets based on the network condition. A decision variable is designed to select red/blue ant for transferring packets based on different network parameters such as bandwidth, energy, mobility, and distance. The selection of red ant means the route where the concentration of red pheromone is maximum and reverse is true for blue ant. This protocol is also concerns about the link failure during packet transmission in a route. Simulation results using OMNET++ show that ACOLBR outperforms ARA, ANTHOCNET, FACO, AODV, DSDV, DSR, CA-ARTT, and MOAODV in terms of load balancing efficiently in the route for data transmission in between source to destination.