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Research note
Mathew D Esona
Centers for Disease Control and Prevention
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4069-947X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective
Rotavirus remains the main causative agent of gastroenteritis in young children in countries that have not yet introduced the vaccine. In Benin, rotavirus vaccine was introduced late December 2019 into the EPI. This study aims to provide pre-vaccination era rotavirus genotyping data in Benin. These data can supplement data from the surveillance system of Ministry of Health of Benin which is supported by the World Health Organization (WHO).
Results
Of the 420 diarrheal stool samples, actively collected in southern Benin from July 2016 through November 2018 from children under five years old and suffering from gastroenteritis, 167 (39.8%) samples were rotavirus EIA positive. 186 (44.3%) samples contained amplifiable rotavirus RNA detected by qRT-PCR method and were genotyped using one-step RT-PCR multiplex genotyping method. G1P[8] represents the predominant genotype (32%) followed by the G2P[4] (26%), G3P[6] (16%), G12P[8] (13%) and mixed G and P types (1%). Four samples (2%) could not be assigned both G and P type specificity.
Keywords
pediatric, rotavirus, surveillance, Genotypes, Benin
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This is a list of supplementary files associated with this preprint. Click to download.
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CURRENT STATUS: Published
View the published article at BMC Research Notes.
Version 4
Posted 12 Jan, 2021
No community comments so far
Submission checks complete
On 07 Oct, 2020
Editorial decision: Accept
On 06 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 29 Sep, 2020
Editor assigned
On 28 Sep, 2020
Submission checks complete
On 27 Sep, 2020
Editor invited
On 27 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 21 Sep, 2020
Editor assigned
On 18 Sep, 2020
Submission checks complete
On 17 Sep, 2020
Editor invited
On 17 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 24 Aug, 2020
Review #1 received
Received 18 Aug, 2020
Reviewer #1 agreed
On 16 Aug, 2020
Editor assigned
On 15 Aug, 2020
Reviewers invited
Invitations sent on 15 Aug, 2020
Submission checks complete
On 14 Aug, 2020
Editor invited
On 14 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
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See the published version of this preprint at BMC Research Notes.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Mathew D Esona
Centers for Disease Control and Prevention
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4069-947X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Research Notes.
Version 4
Posted 12 Jan, 2021
No community comments so far
Submission checks complete
On 07 Oct, 2020
Editorial decision: Accept
On 06 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 29 Sep, 2020
Editor assigned
On 28 Sep, 2020
Submission checks complete
On 27 Sep, 2020
Editor invited
On 27 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 21 Sep, 2020
Editor assigned
On 18 Sep, 2020
Submission checks complete
On 17 Sep, 2020
Editor invited
On 17 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 24 Aug, 2020
Review #1 received
Received 18 Aug, 2020
Reviewer #1 agreed
On 16 Aug, 2020
Editor assigned
On 15 Aug, 2020
Reviewers invited
Invitations sent on 15 Aug, 2020
Submission checks complete
On 14 Aug, 2020
Editor invited
On 14 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Research Notes.
Objective
Rotavirus remains the main causative agent of gastroenteritis in young children in countries that have not yet introduced the vaccine. In Benin, rotavirus vaccine was introduced late December 2019 into the EPI. This study aims to provide pre-vaccination era rotavirus genotyping data in Benin. These data can supplement data from the surveillance system of Ministry of Health of Benin which is supported by the World Health Organization (WHO).
Results
Of the 420 diarrheal stool samples, actively collected in southern Benin from July 2016 through November 2018 from children under five years old and suffering from gastroenteritis, 167 (39.8%) samples were rotavirus EIA positive. 186 (44.3%) samples contained amplifiable rotavirus RNA detected by qRT-PCR method and were genotyped using one-step RT-PCR multiplex genotyping method. G1P[8] represents the predominant genotype (32%) followed by the G2P[4] (26%), G3P[6] (16%), G12P[8] (13%) and mixed G and P types (1%). Four samples (2%) could not be assigned both G and P type specificity.
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
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