Molecular characteristics of rotavirus genotypes circulating in the south of Benin, 2016-2018
Objective
Rotavirus remains the main causative agent of gastroenteritis in young children in countries that have not yet introduced the vaccine. In Benin, rotavirus vaccine was introduced late December 2019 into the EPI. This study aims to provide pre-vaccination era rotavirus genotyping data in Benin. These data can supplement data from the surveillance system of Ministry of Health of Benin which is supported by the World Health Organization (WHO).
Results
Of the 420 diarrheal stool samples, actively collected in southern Benin from July 2016 through November 2018 from children under five years old and suffering from gastroenteritis, 167 (39.8%) samples were rotavirus EIA positive. 186 (44.3%) samples contained amplifiable rotavirus RNA detected by qRT-PCR method and were genotyped using one-step RT-PCR multiplex genotyping method. G1P[8] represents the predominant genotype (32%) followed by the G2P[4] (26%), G3P[6] (16%), G12P[8] (13%) and mixed G and P types (1%). Four samples (2%) could not be assigned both G and P type specificity.
Figure 1
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Posted 12 Jan, 2021
On 19 Oct, 2020
On 07 Oct, 2020
On 06 Oct, 2020
On 29 Sep, 2020
On 28 Sep, 2020
On 27 Sep, 2020
On 27 Sep, 2020
On 21 Sep, 2020
On 18 Sep, 2020
On 17 Sep, 2020
On 17 Sep, 2020
On 24 Aug, 2020
Received 18 Aug, 2020
On 16 Aug, 2020
On 15 Aug, 2020
Invitations sent on 15 Aug, 2020
On 14 Aug, 2020
On 14 Aug, 2020
Posted 30 Jul, 2020
Molecular characteristics of rotavirus genotypes circulating in the south of Benin, 2016-2018
Posted 12 Jan, 2021
On 19 Oct, 2020
On 07 Oct, 2020
On 06 Oct, 2020
On 29 Sep, 2020
On 28 Sep, 2020
On 27 Sep, 2020
On 27 Sep, 2020
On 21 Sep, 2020
On 18 Sep, 2020
On 17 Sep, 2020
On 17 Sep, 2020
On 24 Aug, 2020
Received 18 Aug, 2020
On 16 Aug, 2020
On 15 Aug, 2020
Invitations sent on 15 Aug, 2020
On 14 Aug, 2020
On 14 Aug, 2020
Posted 30 Jul, 2020
Objective
Rotavirus remains the main causative agent of gastroenteritis in young children in countries that have not yet introduced the vaccine. In Benin, rotavirus vaccine was introduced late December 2019 into the EPI. This study aims to provide pre-vaccination era rotavirus genotyping data in Benin. These data can supplement data from the surveillance system of Ministry of Health of Benin which is supported by the World Health Organization (WHO).
Results
Of the 420 diarrheal stool samples, actively collected in southern Benin from July 2016 through November 2018 from children under five years old and suffering from gastroenteritis, 167 (39.8%) samples were rotavirus EIA positive. 186 (44.3%) samples contained amplifiable rotavirus RNA detected by qRT-PCR method and were genotyped using one-step RT-PCR multiplex genotyping method. G1P[8] represents the predominant genotype (32%) followed by the G2P[4] (26%), G3P[6] (16%), G12P[8] (13%) and mixed G and P types (1%). Four samples (2%) could not be assigned both G and P type specificity.
Figure 1