Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines
Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. C onclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis.
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Posted 13 Jan, 2020
On 03 Feb, 2020
On 09 Jan, 2020
On 09 Jan, 2020
On 07 Jan, 2020
Invitations sent on 19 Dec, 2019
On 19 Dec, 2019
On 19 Dec, 2019
On 11 Dec, 2019
On 10 Dec, 2019
On 10 Dec, 2019
Received 23 Nov, 2019
On 23 Nov, 2019
On 11 Oct, 2019
Received 11 Oct, 2019
On 03 Oct, 2019
On 30 Sep, 2019
Invitations sent on 28 Sep, 2019
On 13 Sep, 2019
On 05 Sep, 2019
On 04 Sep, 2019
On 03 Sep, 2019
Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines
Posted 13 Jan, 2020
On 03 Feb, 2020
On 09 Jan, 2020
On 09 Jan, 2020
On 07 Jan, 2020
Invitations sent on 19 Dec, 2019
On 19 Dec, 2019
On 19 Dec, 2019
On 11 Dec, 2019
On 10 Dec, 2019
On 10 Dec, 2019
Received 23 Nov, 2019
On 23 Nov, 2019
On 11 Oct, 2019
Received 11 Oct, 2019
On 03 Oct, 2019
On 30 Sep, 2019
Invitations sent on 28 Sep, 2019
On 13 Sep, 2019
On 05 Sep, 2019
On 04 Sep, 2019
On 03 Sep, 2019
Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. C onclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6