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Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines
Maja Šikić Pogačar
Biotechnical Faculty
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4557-1205
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. C onclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis.
Keywords
Campylobacter jejuni, Lactobacillus spp., Chicken and Pig cell line, Adhesion, Invasion, Translocation
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CURRENT STATUS: Published
Version 3
Posted 13 Jan, 2020
Published
On 03 Feb, 2020
No community comments so far
Submission checks complete
On 09 Jan, 2020
Editorial decision: Accept
On 09 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 07 Jan, 2020
Reviewers invited
Invitations sent on 19 Dec, 2019
Reviewer #1 agreed
On 19 Dec, 2019
Reviewer #2 agreed
On 19 Dec, 2019
Editor assigned
On 11 Dec, 2019
Submission checks complete
On 10 Dec, 2019
Editor invited
On 10 Dec, 2019
No comments provided
Review #2 received
Received 23 Nov, 2019
Editorial decision: Minor revision
On 23 Nov, 2019
Reviewer #3 agreed
On 11 Oct, 2019
Review #1 received
Received 11 Oct, 2019
Reviewer #2 agreed
On 03 Oct, 2019
Reviewer #1 agreed
On 30 Sep, 2019
Reviewers invited
Invitations sent on 28 Sep, 2019
Submission checks complete
On 13 Sep, 2019
Editor assigned
On 05 Sep, 2019
Editor invited
On 04 Sep, 2019
First submitted
On 03 Sep, 2019
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines
Maja Šikić Pogačar
Biotechnical Faculty
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4557-1205
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 13 Jan, 2020
Published
On 03 Feb, 2020
No community comments so far
Submission checks complete
On 09 Jan, 2020
Editorial decision: Accept
On 09 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 07 Jan, 2020
Reviewers invited
Invitations sent on 19 Dec, 2019
Reviewer #1 agreed
On 19 Dec, 2019
Reviewer #2 agreed
On 19 Dec, 2019
Editor assigned
On 11 Dec, 2019
Submission checks complete
On 10 Dec, 2019
Editor invited
On 10 Dec, 2019
No comments provided
Review #2 received
Received 23 Nov, 2019
Editorial decision: Minor revision
On 23 Nov, 2019
Reviewer #3 agreed
On 11 Oct, 2019
Review #1 received
Received 11 Oct, 2019
Reviewer #2 agreed
On 03 Oct, 2019
Reviewer #1 agreed
On 30 Sep, 2019
Reviewers invited
Invitations sent on 28 Sep, 2019
Submission checks complete
On 13 Sep, 2019
Editor assigned
On 05 Sep, 2019
Editor invited
On 04 Sep, 2019
First submitted
On 03 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. C onclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6