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Research article
Chuanjing Li
Xiaogan Hospital Affiliated to Wuhan University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4773-3426
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: miRNAs participate in the development and progression of diabetic retinopathy (DR). High expression of NF-κB signaling pathway boosts the progression of retinopathy in diabetes rats. We found a site where miR-874 bound to the NF-κB p65 by a bioinformatics website. Therefore, we speculated that miR-874 might improve retinopathy in diabetic rats by inhibiting the NF-κB signaling pathway.
Methods: Ten healthy rats were taken as the control group. Sixty streptozotocin (60 mg/kg)-induced diabetes model rats were randomly divided into the model group (injection of normal saline), NC (negative control) agomir group (injection of NC mimic), miR-874 agomir group (injection of miR-874 mimic), miR-874 anti-agomir group (injection of miR-874 inhibitor), EVP4593 group (injection of NF-κB signaling pathway antagonist EVP4593), and miR-874 anti-agomir + EVP4593 group (injection of miR-874 inhibitor and EVP4593). All injection was via caudal vein.
Results: miR-874 could target the degradation of p65. Compared with the control group, model rats had reduced miR-874 expression, increased VEGF and Ang2 protein expressions, lowered end-diastolic velocity and peak systolic velocity of central retinal artery (CRA) and blood velocity of central retinal vein and CRA, heightened plasma viscosity, blood viscosity and erythrocyte sedimentation rate at all shear rates, decreased capillary pericytes, increased vascular endothelial cells, and ascended p65 expression in the retina (all P < 0.05). It showed that pathological changes appeared in the retina of diabetes rats. These indexes would be improved in diabetes rats injected with miR-874 mimic or EVP4593, but deteriorated in those injected with miR-874 inhibitor (all P < 0.05). EVP4593 could alleviate the aggravation of retinopathy that was caused by miR-874 inhibition in diabetes rats.
Conclusions: miR-874 mediates the NF-κB signaling pathway by targeting the degradation of p65 to further improve the retina of diabetes rats, showing the improvement effect of miR-874 on diabetic retinopathy in rats.
Keywords
diabetic retinopathy, Rela (p65), rat, miR-874, NF-κB
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This is a preprint. It has not completed peer review.
Research article
Chuanjing Li
Xiaogan Hospital Affiliated to Wuhan University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4773-3426
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Advances in Clinical and Experimental Medicine.
Version 4
Posted 01 Jun, 2020
No community comments so far
No comments provided
No comments provided
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Endocrinology & Metabolism
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Advances in Clinical and Experimental Medicine.
Background: miRNAs participate in the development and progression of diabetic retinopathy (DR). High expression of NF-κB signaling pathway boosts the progression of retinopathy in diabetes rats. We found a site where miR-874 bound to the NF-κB p65 by a bioinformatics website. Therefore, we speculated that miR-874 might improve retinopathy in diabetic rats by inhibiting the NF-κB signaling pathway.
Methods: Ten healthy rats were taken as the control group. Sixty streptozotocin (60 mg/kg)-induced diabetes model rats were randomly divided into the model group (injection of normal saline), NC (negative control) agomir group (injection of NC mimic), miR-874 agomir group (injection of miR-874 mimic), miR-874 anti-agomir group (injection of miR-874 inhibitor), EVP4593 group (injection of NF-κB signaling pathway antagonist EVP4593), and miR-874 anti-agomir + EVP4593 group (injection of miR-874 inhibitor and EVP4593). All injection was via caudal vein.
Results: miR-874 could target the degradation of p65. Compared with the control group, model rats had reduced miR-874 expression, increased VEGF and Ang2 protein expressions, lowered end-diastolic velocity and peak systolic velocity of central retinal artery (CRA) and blood velocity of central retinal vein and CRA, heightened plasma viscosity, blood viscosity and erythrocyte sedimentation rate at all shear rates, decreased capillary pericytes, increased vascular endothelial cells, and ascended p65 expression in the retina (all P < 0.05). It showed that pathological changes appeared in the retina of diabetes rats. These indexes would be improved in diabetes rats injected with miR-874 mimic or EVP4593, but deteriorated in those injected with miR-874 inhibitor (all P < 0.05). EVP4593 could alleviate the aggravation of retinopathy that was caused by miR-874 inhibition in diabetes rats.
Conclusions: miR-874 mediates the NF-κB signaling pathway by targeting the degradation of p65 to further improve the retina of diabetes rats, showing the improvement effect of miR-874 on diabetic retinopathy in rats.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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