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Research article
Junhe Hu
Hunan University of Humanities, Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9013-8171
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Follicular development is crucial to normal oocyte maturation, with follicular size closely related to oocyte maturation. To better understand the molecular mechanisms behind porcine oocyte maturation, we obtained exosomal miRNA from porcine follicular fluid (PFF). These miRNA samples were then sequenced and analyzed regarding their different follicular sizes, as described in the methods section.
First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads from the PFF exosomal sequencing data were successfully mapped to the porcine genome database. Second, we used hierarchical clustering methods to determine that significantly expressed miRNAs were clustered into A, B, C, and D groups in our heatmap according to different follicle sizes. These results allowed for the targeting of potential mRNAs genes related to porcine oocyte development. Third, we chose ten, significantly expressed miRNAs and predicted their target genes for further GO analysis. These results showed that the expression levels of neurotransmitter secretion genes were greatly changed, as were many target genes involved in the regulation of FSH secretion. Notably, these are genes that are very closely related to oocyte maturation in growing follicles. We then used pathway analysis for these targeted genes based on the originally selected ten miRNAs. Results indicated that the pathways were mainly related to the biosynthesis of TGF-beta and its signaling pathway, which are very closely related to reproductive system functions.
Finally, these exosomal miRNAs obtained from PFF may provide a valuable addition to our understanding of the mechanism of porcine oocyte maturation. It is also likely that these exosomal miRNAs could function as molecular biomarkers to choose high-quality oocytes and allow for in vitro porcine embryo production.
Keywords
Exosomes, porcine follicular fluid (PFF), oocyte maturation, follicular development, miRNA
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CURRENT STATUS: Published
View the published article at BMC Veterinary Research.
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Submission checks complete
On 06 Dec, 2020
Editorial decision: Accept
On 03 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 30 Nov, 2020
Review #2 received
Received 22 Nov, 2020
Review #1 received
Received 02 Nov, 2020
Reviewer #2 agreed
On 31 Oct, 2020
Reviewers invited
Invitations sent on 29 Oct, 2020
Reviewer #1 agreed
On 29 Oct, 2020
Editor assigned
On 28 Oct, 2020
Submission checks complete
On 27 Oct, 2020
Editor invited
On 27 Oct, 2020
Version 1
Posted 31 Aug, 2020
No comments provided
Editorial decision: Major revision
On 16 Oct, 2020
Review #2 received
Received 13 Oct, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Review #1 received
Received 24 Sep, 2020
Reviewer #1 agreed
On 04 Sep, 2020
Reviewers invited
Invitations sent on 03 Sep, 2020
Editor assigned
On 01 Sep, 2020
Submission checks complete
On 28 Aug, 2020
Editor invited
On 27 Aug, 2020
First submitted
On 04 Aug, 2020
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A new preprint design is coming!
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See the published version of this preprint at BMC Veterinary Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Junhe Hu
Hunan University of Humanities, Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9013-8171
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Veterinary Research.
No community comments so far
Submission checks complete
On 06 Dec, 2020
Editorial decision: Accept
On 03 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 30 Nov, 2020
Review #2 received
Received 22 Nov, 2020
Review #1 received
Received 02 Nov, 2020
Reviewer #2 agreed
On 31 Oct, 2020
Reviewers invited
Invitations sent on 29 Oct, 2020
Reviewer #1 agreed
On 29 Oct, 2020
Editor assigned
On 28 Oct, 2020
Submission checks complete
On 27 Oct, 2020
Editor invited
On 27 Oct, 2020
Version 1
Posted 31 Aug, 2020
No comments provided
Editorial decision: Major revision
On 16 Oct, 2020
Review #2 received
Received 13 Oct, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Review #1 received
Received 24 Sep, 2020
Reviewer #1 agreed
On 04 Sep, 2020
Reviewers invited
Invitations sent on 03 Sep, 2020
Editor assigned
On 01 Sep, 2020
Submission checks complete
On 28 Aug, 2020
Editor invited
On 27 Aug, 2020
First submitted
On 04 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Veterinary Research.
Follicular development is crucial to normal oocyte maturation, with follicular size closely related to oocyte maturation. To better understand the molecular mechanisms behind porcine oocyte maturation, we obtained exosomal miRNA from porcine follicular fluid (PFF). These miRNA samples were then sequenced and analyzed regarding their different follicular sizes, as described in the methods section.
First, these results showed that this process successfully isolated PFF exosomes. Nearly all valid reads from the PFF exosomal sequencing data were successfully mapped to the porcine genome database. Second, we used hierarchical clustering methods to determine that significantly expressed miRNAs were clustered into A, B, C, and D groups in our heatmap according to different follicle sizes. These results allowed for the targeting of potential mRNAs genes related to porcine oocyte development. Third, we chose ten, significantly expressed miRNAs and predicted their target genes for further GO analysis. These results showed that the expression levels of neurotransmitter secretion genes were greatly changed, as were many target genes involved in the regulation of FSH secretion. Notably, these are genes that are very closely related to oocyte maturation in growing follicles. We then used pathway analysis for these targeted genes based on the originally selected ten miRNAs. Results indicated that the pathways were mainly related to the biosynthesis of TGF-beta and its signaling pathway, which are very closely related to reproductive system functions.
Finally, these exosomal miRNAs obtained from PFF may provide a valuable addition to our understanding of the mechanism of porcine oocyte maturation. It is also likely that these exosomal miRNAs could function as molecular biomarkers to choose high-quality oocytes and allow for in vitro porcine embryo production.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
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