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Production and identification of two antifungal terpenoids from the Posidonia oceanica epiphytic Ascomycota Mariannaea humicola IG100.
Marcella Pasqualetti
Universita degli Studi della Tuscia
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2032-5596
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Microbial Cell Factories.
Background: Marine fungi are an important repository of bioactive molecules with great potential in different technological fields, the annual number of new compounds isolated from marine fungi is impressive and the general trend indicates that it is still on the rise. In this context, the antifungal and antimicrobial activity of the marine strain Mariannaea humicola IG100 was evaluated and two active terpenoids were isolated and characterized.
Methods: Preliminary screening of activity of marine strain IG100 was carried out by agar plug diffusion methods against fungal (P. griseofulvum TSF04) and bacterial (Bacillus pumilus KB66 and Escherichia coli JM109) strains. Subsequently, inhibition tests were done by using the cultural broth and the organic extract (ethyl acetate, EtOAc) by the agar well diffusion methods. The main active fractions were identified and tested for their antifungal activity against P. griseofulvum TSF04 in a 24 wells microplate at different concentrations (1000, 100, 10 and 1.0 µg/mL). Two active compounds were characterized and their relative MIC measured by the broth micro-dilution methods in a 96-well microplate against A. flavus IG133, P. griseofulvum TSF04, and T. pleuroticola IG137.
Results: Marine strain IG100 presented significant antifungal activity associated with two active compounds, the terpenoids terperstacin 1 and 19-acetyl-4-hydroxydictyodiol 2. Their MIC values were measured for Aspergillus flavus (MIC of 7.9 µg/mL and 31.3 µg/mL for 1 and 2, respectively), P. griseofulvum (MIC of 25 µg/mL and 100 µg/mL for 1 and 2, respectively) and T. pleuroticola (MIC >500 µg/mL and 125 µg/mL for 1 and 2, respectively). They showed a rather good fungistatic effect.
Conclusions: In this study, the first marine strain of M. humicola (IG100) was investigated for the production of bioactive molecules. Strain IG100 produced significant amounts of two bioactive terpenoids, terperstacin 1 and 19-acetyl-4-hydroxydictyodiol 2. The two compounds showed significant antifungal activities against A. flavus IG133, T. pleuroticola IG137 and P. griseofulvum TSF04. Compound 2 was identified for the first time in fungi.
Keywords
Mariannaea humicola, marine fungi, antifungal activity, terpestacin, fungal xenicanes
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CURRENT STATUS: Published
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Posted 22 Sep, 2020
Published
On 01 Oct, 2020
No community comments so far
Editorial decision: Accept
On 23 Sep, 2020
Editor assigned
On 21 Sep, 2020
Submission checks complete
On 20 Sep, 2020
Editor invited
On 20 Sep, 2020
No comments provided
Review #2 received
Received 01 Sep, 2020
Editorial decision: Minor Revision
On 01 Sep, 2020
Review #1 received
Received 18 Aug, 2020
Reviewer #2 agreed
On 17 Aug, 2020
Reviewers invited
Invitations sent on 15 Aug, 2020
Reviewer #1 agreed
On 15 Aug, 2020
Editor assigned
On 05 Aug, 2020
First submitted
On 04 Aug, 2020
Submission checks complete
On 04 Aug, 2020
Editor invited
On 04 Aug, 2020
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Subject Areas
Applied & Industrial Microbiology
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This preprint is under consideration at Microbial Cell Factories. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Production and identification of two antifungal terpenoids from the Posidonia oceanica epiphytic Ascomycota Mariannaea humicola IG100.
Marcella Pasqualetti
Universita degli Studi della Tuscia
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2032-5596
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 22 Sep, 2020
Published
On 01 Oct, 2020
No community comments so far
Editorial decision: Accept
On 23 Sep, 2020
Editor assigned
On 21 Sep, 2020
Submission checks complete
On 20 Sep, 2020
Editor invited
On 20 Sep, 2020
No comments provided
Review #2 received
Received 01 Sep, 2020
Editorial decision: Minor Revision
On 01 Sep, 2020
Review #1 received
Received 18 Aug, 2020
Reviewer #2 agreed
On 17 Aug, 2020
Reviewers invited
Invitations sent on 15 Aug, 2020
Reviewer #1 agreed
On 15 Aug, 2020
Editor assigned
On 05 Aug, 2020
First submitted
On 04 Aug, 2020
Submission checks complete
On 04 Aug, 2020
Editor invited
On 04 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Microbial Cell Factories.
Background: Marine fungi are an important repository of bioactive molecules with great potential in different technological fields, the annual number of new compounds isolated from marine fungi is impressive and the general trend indicates that it is still on the rise. In this context, the antifungal and antimicrobial activity of the marine strain Mariannaea humicola IG100 was evaluated and two active terpenoids were isolated and characterized.
Methods: Preliminary screening of activity of marine strain IG100 was carried out by agar plug diffusion methods against fungal (P. griseofulvum TSF04) and bacterial (Bacillus pumilus KB66 and Escherichia coli JM109) strains. Subsequently, inhibition tests were done by using the cultural broth and the organic extract (ethyl acetate, EtOAc) by the agar well diffusion methods. The main active fractions were identified and tested for their antifungal activity against P. griseofulvum TSF04 in a 24 wells microplate at different concentrations (1000, 100, 10 and 1.0 µg/mL). Two active compounds were characterized and their relative MIC measured by the broth micro-dilution methods in a 96-well microplate against A. flavus IG133, P. griseofulvum TSF04, and T. pleuroticola IG137.
Results: Marine strain IG100 presented significant antifungal activity associated with two active compounds, the terpenoids terperstacin 1 and 19-acetyl-4-hydroxydictyodiol 2. Their MIC values were measured for Aspergillus flavus (MIC of 7.9 µg/mL and 31.3 µg/mL for 1 and 2, respectively), P. griseofulvum (MIC of 25 µg/mL and 100 µg/mL for 1 and 2, respectively) and T. pleuroticola (MIC >500 µg/mL and 125 µg/mL for 1 and 2, respectively). They showed a rather good fungistatic effect.
Conclusions: In this study, the first marine strain of M. humicola (IG100) was investigated for the production of bioactive molecules. Strain IG100 produced significant amounts of two bioactive terpenoids, terperstacin 1 and 19-acetyl-4-hydroxydictyodiol 2. The two compounds showed significant antifungal activities against A. flavus IG133, T. pleuroticola IG137 and P. griseofulvum TSF04. Compound 2 was identified for the first time in fungi.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5