CC is one of the most frequent gynecologic malignancies in female patients globally, and the incidence rate exists obvious differences between regions. In recent years, with the prevalence of sexually transmitted diseases and their induction factors, such as human papillomavirus (HPV), CC becomes a heavy burden for female human beings. Besides, the onset ages of CC patients become younger and younger. What’s more, CC is reported to be the only malignancy that could be preventable and curative if it was found at early stages. Therefore, early diagnosis and timely treatment are of great importance to improve the survival rate of CC patients. Recently, tumor biomarkers, such as chain antigen 19-9 (CA19-9), carcino-embryonic antigen (CEA) and cancer antigen 125 (CA125) are more and more used in the diagnosis and prognosis of various diseases, including CC [23, 24]. However, the biomarkers are lack specificity for CC. So, we attempted to find some specific and effective candidates for CC.
LncRNAs, which are becoming more and more prevalent in the life science research in recent years, have been demonstrated to be potential targets in detection and treatment of cancers . It has been confirmed that there are thousands of lncRNAs in mammalian genomes . And plenty of lncRNAs have been investigated in various malignant diseases. For example, Ji et al. have reported that lncRNA TUG1 was involved in the cell transference and invasion in gastric cancer through regulating miR-144/c-Met axis . Yang et al. claimed that lncRNA GAPLINC enhanced invasion of colorectal cancer via binding to PSF and NONO . These studies indicated that lncRNAs might be related with the development and progression of malignancies. PVT1, a lncRNA, as mentioned previously, was observed to be elevated in various cancers. And Iden et al. also found that PVT1 was increased in CC tissues compared with healthy controls . Thereby, we were engaged in exploring the role of PVT1 in CC diagnosis in this study.
We first measured the expression of PVT1 mRNA in CC samples and healthy controls using the qRT-PCR method. And we observed that the PTV1 mRNA expression was significantly high in CC samples compared with healthy controls, which agreed with and confirmed the previous results. The following Chi-square test showed that elevated expression of PVT1 was significantly contributed to large tumor size, positive uterus infiltration and advanced FIGO stages. All the above results suggested that PVT1 expression might be related with the occurrence and development of CC. So we further detected the link between PVT1 expression and CC diagnosis. The final ROC analysis illustrated that high expression of PVT1 could be regarded as a potential diagnostic marker for CC with high sensitivity and specificity.
Compared with miRNAs, the structure of lncRNAs is more complex, which leads to the diverse action modes of lncRNAs on tumors and genes, and more subtle regulation of the life processes. So far, the precise mechanism of PVT1 on CC tumorigenesis is still not well understood. In fact, multiple studies have provided the mechanistic data of PVT1 and suggested that it exerts its effect in a cell-type or disease-specific manner. For example, PVT1 is reported to locate at about 55 kb downstream of the well known oncogene MYC, and is found to share close relationships with MYC in various cancers [29, 30]. Thus , we sought to be better define the role of PVT1 in CC. This finding could provide a novel research area for our further studies.