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Research
Electroacupuncture attenuates depressive disorder and gastrointestinal dysfunction in myocardial hypertrophy via upregulation of 5-HT on Rats
Hua Wang
Hubei University of Chinese Medicine
Corresponding Author
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Background: Acupuncture therapy has been described as a preventive and therapeutic technique with low or no risk for a number of conditions related to cardiovascular disease. However, its role in protecting against myocardial hypertrophy (MH) has not been elucidated. This study aimed to investigate whether electroacupuncture (EA) can attenuate MH by upregulating tryptophan 5-hydroxytryptamine (5-HT) metabolism, depressive disorder and gastrointestinal dysfunction in MH rats model.
Methods: We assessed the anti-myocardial ischemia effects of EA by using the isoproterenol hydrochloride (ISO)-induced myocardial hypertrophy (MH) rats model. Forty 3-month-old Sprague-Dawley (SD) rats were randomly divided into normal, model, EA and sham EA groups, with ten rats in each group. Regression of MH was evaluated by ratio of heart weight/body weight (HW/BW) and ratio of left ventricular weight/body weight (LVW/BW). Ultrastructural observation and hematoxylin and eosin (H&E) staining were evaluated to cardiac pathology. The sucrose preference test (SPT), open field test (OFT), and forced swim test (FSW) were conducted to examine the depression-like behavior. Gastric retention and intestinal transit were evaluated using the carbon powder labeling method, while immunohistochemical staining was used to detect 5-HT expression in the cerebral cortex and ileum.
Key findings: EA therapy alleviates rats’ depression-like symptoms. For example, the rate of sucrose solution consumption was higher, the total and central distance traveled in the open field test were increased, and immobility time was decreased in these rats by EA therapy. Furthermore, swimming, struggling and latency to immobility were increased in the forced swim test as well. Expression of 5-HT was increased in the cerebral cortex and ileum after EA treatment.
Conclusion: EA improved the ischemic and depressed symptoms, as well as gastrointestinal dysfunction of MH rats model induced by ISO, which may related with 5-HT metabolism.
Keywords
Myocardial hypertrophy, Electroacupucture, 5-hydroxytryptamine, Gastrointestinal motility
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This is a preprint. It has not completed peer review.
Research
Electroacupuncture attenuates depressive disorder and gastrointestinal dysfunction in myocardial hypertrophy via upregulation of 5-HT on Rats
Hua Wang
Hubei University of Chinese Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Aug, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Gastroenterology & Hepatology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Acupuncture therapy has been described as a preventive and therapeutic technique with low or no risk for a number of conditions related to cardiovascular disease. However, its role in protecting against myocardial hypertrophy (MH) has not been elucidated. This study aimed to investigate whether electroacupuncture (EA) can attenuate MH by upregulating tryptophan 5-hydroxytryptamine (5-HT) metabolism, depressive disorder and gastrointestinal dysfunction in MH rats model.
Methods: We assessed the anti-myocardial ischemia effects of EA by using the isoproterenol hydrochloride (ISO)-induced myocardial hypertrophy (MH) rats model. Forty 3-month-old Sprague-Dawley (SD) rats were randomly divided into normal, model, EA and sham EA groups, with ten rats in each group. Regression of MH was evaluated by ratio of heart weight/body weight (HW/BW) and ratio of left ventricular weight/body weight (LVW/BW). Ultrastructural observation and hematoxylin and eosin (H&E) staining were evaluated to cardiac pathology. The sucrose preference test (SPT), open field test (OFT), and forced swim test (FSW) were conducted to examine the depression-like behavior. Gastric retention and intestinal transit were evaluated using the carbon powder labeling method, while immunohistochemical staining was used to detect 5-HT expression in the cerebral cortex and ileum.
Key findings: EA therapy alleviates rats’ depression-like symptoms. For example, the rate of sucrose solution consumption was higher, the total and central distance traveled in the open field test were increased, and immobility time was decreased in these rats by EA therapy. Furthermore, swimming, struggling and latency to immobility were increased in the forced swim test as well. Expression of 5-HT was increased in the cerebral cortex and ileum after EA treatment.
Conclusion: EA improved the ischemic and depressed symptoms, as well as gastrointestinal dysfunction of MH rats model induced by ISO, which may related with 5-HT metabolism.
Figure 1
Figure 2
Figure 3
Figure 4