Naturally occurring phytochemical compounds have received considerable attention as alternative candidates for anti-amyloidogenic agents. This study, utilizing human insulin and amyloid beta peptide as an in vitro model, determined the anti-amyloid effects of alkaloids extracted derived from Stephania venosa. Alkaloids extracts including crebanine, O-methylbulbocapnine, tetrahydropalmatine and N-methyltetrahydropalmatine were used. Inhibition of amyloid protein aggregation were studied by fluorescence spectroscopy. Most alkaloids, except N-methyltetrahydropalmatine, exhibited the inhibitory properties against amyloid fibrillation either insulin or amyloid-beta peptide. Among alkaloids group, crebanine and tetrahydropalmatine showed the potent properties of anti-amyloidogenesis. These results suggest that alkaloids could be used as a natural compound for the development of drugs against amyloid protein aggregation for treatment of amyloid-related diseases.