Background: The newly established mouse cortical bone–derived stem cells (mCBSCs) are unique stem cells compared with mouse mesenchymal stem cells (mMSCs), and can improve cardiac function after myocardial infarction. However, the mCBSCs’ characterizations including their stem cell features, non-cardiac therapeutic potential, and cell surface features have not been fully understood. In this study, we examined stem cell features, cell surface glycan profiles, and cell functional features in mCBSCs compared to the bone marrow-derived mMSCs.
Methods: The stem cell features were compared between mCBSCs and mMSCs by immunoblotting of stem cell markers, self-renewal assay, and multilineage differentiation. The cell surface glycan profiles were examined by lectin array analysis and fluorescence-activated cell sorting analysis using lectins. The production of transforming growth factor (TGF)-β1 from mCBSCs were examined by ELISA. The effects of TGF-β1 released from mCBSCs on self-migration and on activation of fibroblast were examined by migration assay and immunocytostaining, respectively.
Results: The stem cell feature, including the self-renewing ability in mCBSCs was higher than that in mMSCs. In contrast, the differentiation ability of mCBSCs was limited to the chondrogenic lineage among three types of cells (adipocyte, osteoblast, chondrocyte). The cell surface glycan profiles revealed that α2-6sialic acid is expressed at very low levels on the cell surface of mCBSCs compared with that on mMSCs. Additionally, the lactosamine (Galβ1-4GlcNAc)-structure, poly lactosamine- or poly N-acetylglucosamine-structure, and α2-3sialic acid on both N- and O-glycans are more highly expressed in mCBSCs compared with mMSCs. Furthermore, these highly expressed glycans were increased with cellular aging of mCBSCs. We found that TGF-β1 was released from mCBSCs and the released TGF-β1 contributed to the self-migration of mCBSCs and activation of fibroblasts.
Conclusions: These results reveal the differences between mCBSCs and mMSCs, and it is proposed that there is the potential use of mCBSCs for infarct healing and wound healing.