Effect of gga-miR-155 on Cell Proliferation, Apoptosis and invasion of Marek’s disease Virus (MDV) transformed cell line MSB1 by targeting RORA
Background Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms.Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells.Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.
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Posted 13 Jan, 2020
On 28 Jan, 2020
On 09 Jan, 2020
On 09 Jan, 2020
On 06 Jan, 2020
On 02 Jan, 2020
On 01 Jan, 2020
On 01 Jan, 2020
On 27 Dec, 2019
On 25 Dec, 2019
On 24 Dec, 2019
On 24 Dec, 2019
Received 03 Dec, 2019
On 03 Dec, 2019
On 21 Nov, 2019
Received 28 Oct, 2019
On 28 Oct, 2019
Invitations sent on 07 Oct, 2019
On 25 Sep, 2019
On 25 Sep, 2019
On 24 Sep, 2019
On 23 Sep, 2019
Effect of gga-miR-155 on Cell Proliferation, Apoptosis and invasion of Marek’s disease Virus (MDV) transformed cell line MSB1 by targeting RORA
Posted 13 Jan, 2020
On 28 Jan, 2020
On 09 Jan, 2020
On 09 Jan, 2020
On 06 Jan, 2020
On 02 Jan, 2020
On 01 Jan, 2020
On 01 Jan, 2020
On 27 Dec, 2019
On 25 Dec, 2019
On 24 Dec, 2019
On 24 Dec, 2019
Received 03 Dec, 2019
On 03 Dec, 2019
On 21 Nov, 2019
Received 28 Oct, 2019
On 28 Oct, 2019
Invitations sent on 07 Oct, 2019
On 25 Sep, 2019
On 25 Sep, 2019
On 24 Sep, 2019
On 23 Sep, 2019
Background Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms.Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells.Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6