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Research article
Effect of gga-miR-155 on Cell Proliferation, Apoptosis and invasion of Marek’s disease Virus (MDV) transformed cell line MSB1 by targeting RORA
Zuhua Yu
Henan University of Science and Technology
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms.Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells.Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.
Keywords
gga-miR-155; Marek's disease; MSB1; lymphocyte line; proliferation; apoptosis; RORA
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CURRENT STATUS: Published
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Posted 13 Jan, 2020
Published
On 28 Jan, 2020
No community comments so far
Submission checks complete
On 09 Jan, 2020
Editorial decision: Accept
On 09 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 06 Jan, 2020
Editor assigned
On 02 Jan, 2020
Submission checks complete
On 01 Jan, 2020
Editor invited
On 01 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 27 Dec, 2019
Editor assigned
On 25 Dec, 2019
Submission checks complete
On 24 Dec, 2019
Editor invited
On 24 Dec, 2019
No comments provided
Review #2 received
Received 03 Dec, 2019
Editorial decision: Minor revision
On 03 Dec, 2019
Reviewer #2 agreed
On 21 Nov, 2019
Review #1 received
Received 28 Oct, 2019
Reviewer #1 agreed
On 28 Oct, 2019
Reviewers invited
Invitations sent on 07 Oct, 2019
Submission checks complete
On 25 Sep, 2019
Editor assigned
On 25 Sep, 2019
Editor invited
On 24 Sep, 2019
First submitted
On 23 Sep, 2019
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Effect of gga-miR-155 on Cell Proliferation, Apoptosis and invasion of Marek’s disease Virus (MDV) transformed cell line MSB1 by targeting RORA
Zuhua Yu
Henan University of Science and Technology
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 4
Posted 13 Jan, 2020
Published
On 28 Jan, 2020
No community comments so far
Submission checks complete
On 09 Jan, 2020
Editorial decision: Accept
On 09 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 06 Jan, 2020
Editor assigned
On 02 Jan, 2020
Submission checks complete
On 01 Jan, 2020
Editor invited
On 01 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 27 Dec, 2019
Editor assigned
On 25 Dec, 2019
Submission checks complete
On 24 Dec, 2019
Editor invited
On 24 Dec, 2019
No comments provided
Review #2 received
Received 03 Dec, 2019
Editorial decision: Minor revision
On 03 Dec, 2019
Reviewer #2 agreed
On 21 Nov, 2019
Review #1 received
Received 28 Oct, 2019
Reviewer #1 agreed
On 28 Oct, 2019
Reviewers invited
Invitations sent on 07 Oct, 2019
Submission checks complete
On 25 Sep, 2019
Editor assigned
On 25 Sep, 2019
Editor invited
On 24 Sep, 2019
First submitted
On 23 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background Marek's disease (MD) is an avian lymphoproliferative neoplasm caused by the oncogenic Marek's disease virus (MDV). MicroRNAs (miRNAs) act as oncogenes or tumor suppressors in most cancers. The gga-miR-155 is downregulated in the MDV-infected chicken tissues or lymphocyte lines, although its exact role in tumorigenesis remains unclear. The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms.Results The expression level of gga-miR-155 was manipulated in MSB1 cells using specific mimics and inhibitors. While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. However, its downregulation had the opposite effects. Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells.Conclusion The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6