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Honggang Sun
Research Institute of Subtropical Forestry Chinese Academy of Forestry
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7400-6518
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Forest Ecosystems.
Background: The relationship between the parental tree’ physiological age and the clonal offspring’s lifespan is unclear. White oak (Quercus fabri Hance) has a high sprouting capability after harvest, with the regenerated sprouts being typical clonal individuals. To determine whether regenerated sprouts undergo rapid senescence compared with the parent, the senescence levels of 5-, 10-, 20- and 40-year-old regenerated stump sprouts in a natural forest were evaluated. The antioxidative abilities and transcriptomes in these leaves and shoots were compared.
Results: Older regenerated sprouts still had robust antioxidative systems, with 40-year-old sprouts having lower peroxidation product levels but similar antioxidative enzyme activity levels compared with 5-year-old sprouts. Older leaves had greater transcriptional activities in pathways related to cell growth and division than younger leaves. However, older sprouts had a few unhealthy characteristics, such as increased base excision repair levels and upregulated phagosome, proteasome and glycerophospholipid metabolism pathways in 40-year-old leaves, indicating that DNA damage and tissue remodeling occurred more frequently than in younger leaves. Additionally, plant–pathogen interactions and MAPK signals pathways were upregulated in older shoots, indicating that older shoots suffered from more pathogen-related biotic stress.
Conclusions: The 40-year-old sprouts still had the same vitality level as the 5-year-old sprouts, although the former had some unhealthy characteristics. We conclude that during their first 40 years of growth regenerated stump sprouts do not begin to senesce, and that the parental tree’s physiological age does not significantly affect its clonal offspring’s lifespan.
Keywords
Sprout, regeneration, senescence, White Oak, vegetative reproduction
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CURRENT STATUS: Published
Published
On 07 Feb, 2021
No community comments so far
Editorial decision: Accept
On 07 Jan, 2021
Editor assigned
On 04 Jan, 2021
Submission checks complete
On 04 Jan, 2021
Editor invited
On 04 Jan, 2021
Version 2
Posted 21 Dec, 2020
No comments provided
Editorial decision: Minor Revision
On 23 Dec, 2020
Reviewer #2 agreed
On 15 Dec, 2020
Review #1 received
Received 14 Dec, 2020
Reviewer #1 agreed
On 13 Dec, 2020
Reviewers invited
Invitations sent on 11 Dec, 2020
Editor assigned
On 08 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
No comments provided
Editorial decision: Major Revision
On 03 Nov, 2020
Review #2 received
Received 01 Nov, 2020
Review #1 received
Received 18 Oct, 2020
Reviewer #2 agreed
On 09 Oct, 2020
Reviewer #1 agreed
On 29 Sep, 2020
Reviewers invited
Invitations sent on 17 Sep, 2020
Editor assigned
On 25 Aug, 2020
Submission checks complete
On 24 Aug, 2020
Editor invited
On 24 Aug, 2020
First submitted
On 23 Aug, 2020
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Subject Areas
Forestry
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This preprint is under consideration at Forest Ecosystems. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Honggang Sun
Research Institute of Subtropical Forestry Chinese Academy of Forestry
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7400-6518
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Published
On 07 Feb, 2021
No community comments so far
Editorial decision: Accept
On 07 Jan, 2021
Editor assigned
On 04 Jan, 2021
Submission checks complete
On 04 Jan, 2021
Editor invited
On 04 Jan, 2021
Version 2
Posted 21 Dec, 2020
No comments provided
Editorial decision: Minor Revision
On 23 Dec, 2020
Reviewer #2 agreed
On 15 Dec, 2020
Review #1 received
Received 14 Dec, 2020
Reviewer #1 agreed
On 13 Dec, 2020
Reviewers invited
Invitations sent on 11 Dec, 2020
Editor assigned
On 08 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
No comments provided
Editorial decision: Major Revision
On 03 Nov, 2020
Review #2 received
Received 01 Nov, 2020
Review #1 received
Received 18 Oct, 2020
Reviewer #2 agreed
On 09 Oct, 2020
Reviewer #1 agreed
On 29 Sep, 2020
Reviewers invited
Invitations sent on 17 Sep, 2020
Editor assigned
On 25 Aug, 2020
Submission checks complete
On 24 Aug, 2020
Editor invited
On 24 Aug, 2020
First submitted
On 23 Aug, 2020
Subject Areas
Forestry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Forest Ecosystems.
Background: The relationship between the parental tree’ physiological age and the clonal offspring’s lifespan is unclear. White oak (Quercus fabri Hance) has a high sprouting capability after harvest, with the regenerated sprouts being typical clonal individuals. To determine whether regenerated sprouts undergo rapid senescence compared with the parent, the senescence levels of 5-, 10-, 20- and 40-year-old regenerated stump sprouts in a natural forest were evaluated. The antioxidative abilities and transcriptomes in these leaves and shoots were compared.
Results: Older regenerated sprouts still had robust antioxidative systems, with 40-year-old sprouts having lower peroxidation product levels but similar antioxidative enzyme activity levels compared with 5-year-old sprouts. Older leaves had greater transcriptional activities in pathways related to cell growth and division than younger leaves. However, older sprouts had a few unhealthy characteristics, such as increased base excision repair levels and upregulated phagosome, proteasome and glycerophospholipid metabolism pathways in 40-year-old leaves, indicating that DNA damage and tissue remodeling occurred more frequently than in younger leaves. Additionally, plant–pathogen interactions and MAPK signals pathways were upregulated in older shoots, indicating that older shoots suffered from more pathogen-related biotic stress.
Conclusions: The 40-year-old sprouts still had the same vitality level as the 5-year-old sprouts, although the former had some unhealthy characteristics. We conclude that during their first 40 years of growth regenerated stump sprouts do not begin to senesce, and that the parental tree’s physiological age does not significantly affect its clonal offspring’s lifespan.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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