Ectopic expression of citrus UDP-GLUCOSYL TRANSFERASE gene enhances anthocyanin and proanthocyanidins contents and confers high light tolerance in Arabidopsis
Background Citrus fruits are consumed freshly or as juice to directly provide various dietary flavonoids to humans. Diverse metabolites at different levels are present among Citrus genera and many flavonoids biosynthetic genes were induced after abiotic stresses. To better understand the underlying mechanism, we designed experiments to overexpress a UDP-GLUCOSYL TRANSFERASE gene from Citrus sinensis (sweet orange) to evaluate its possible function in metabolism and response to stress.Results Our results demonstrated that overexpression of Cs-UGT78D3 resulted in high accumulation of proanthocyanidins in the seed coat and a dark brown color to transgenic Arabidopsis seeds. In addition, the total contents of flavonoid and anthocyanin were significantly enhanced in the leaves of overexpressed lines. Gene expression analyses indicated that many flavonoid (flavonol) and anthocyanin genes were up-regulated by 4-15 folds in transgenic Arabidopsis. Moreover, after 14 days of high light stress treatment, the transgenic Arabidopsis lines showed strong antioxidant activity and higher total contents of anthocyanins and flavonoids in leaves compared with the wild type.Conclusion Our study concluded that the citrus Cs-UGT78D3 gene is involved in proanthocyanidins accumulation in seed coats and confers tolerance to high light stress by accumulating the total anthocyanin and flavonoid contents with better antioxidant potential (due to photoprotective activity of anthocyanin) in the transgenic Arabidopsis.
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Posted 20 Dec, 2019
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On 02 Dec, 2019
On 01 Dec, 2019
On 01 Dec, 2019
Received 30 Oct, 2019
On 30 Oct, 2019
On 16 Oct, 2019
On 15 Oct, 2019
Received 15 Oct, 2019
Invitations sent on 10 Oct, 2019
On 29 Sep, 2019
On 24 Sep, 2019
On 23 Sep, 2019
On 19 Sep, 2019
Ectopic expression of citrus UDP-GLUCOSYL TRANSFERASE gene enhances anthocyanin and proanthocyanidins contents and confers high light tolerance in Arabidopsis
Posted 20 Dec, 2019
On 30 Dec, 2019
On 19 Dec, 2019
On 18 Dec, 2019
On 17 Dec, 2019
On 17 Dec, 2019
On 17 Dec, 2019
On 17 Dec, 2019
On 11 Dec, 2019
On 10 Dec, 2019
On 04 Dec, 2019
Received 03 Dec, 2019
On 03 Dec, 2019
Received 03 Dec, 2019
On 02 Dec, 2019
Invitations sent on 02 Dec, 2019
On 02 Dec, 2019
On 01 Dec, 2019
On 01 Dec, 2019
Received 30 Oct, 2019
On 30 Oct, 2019
On 16 Oct, 2019
On 15 Oct, 2019
Received 15 Oct, 2019
Invitations sent on 10 Oct, 2019
On 29 Sep, 2019
On 24 Sep, 2019
On 23 Sep, 2019
On 19 Sep, 2019
Background Citrus fruits are consumed freshly or as juice to directly provide various dietary flavonoids to humans. Diverse metabolites at different levels are present among Citrus genera and many flavonoids biosynthetic genes were induced after abiotic stresses. To better understand the underlying mechanism, we designed experiments to overexpress a UDP-GLUCOSYL TRANSFERASE gene from Citrus sinensis (sweet orange) to evaluate its possible function in metabolism and response to stress.Results Our results demonstrated that overexpression of Cs-UGT78D3 resulted in high accumulation of proanthocyanidins in the seed coat and a dark brown color to transgenic Arabidopsis seeds. In addition, the total contents of flavonoid and anthocyanin were significantly enhanced in the leaves of overexpressed lines. Gene expression analyses indicated that many flavonoid (flavonol) and anthocyanin genes were up-regulated by 4-15 folds in transgenic Arabidopsis. Moreover, after 14 days of high light stress treatment, the transgenic Arabidopsis lines showed strong antioxidant activity and higher total contents of anthocyanins and flavonoids in leaves compared with the wild type.Conclusion Our study concluded that the citrus Cs-UGT78D3 gene is involved in proanthocyanidins accumulation in seed coats and confers tolerance to high light stress by accumulating the total anthocyanin and flavonoid contents with better antioxidant potential (due to photoprotective activity of anthocyanin) in the transgenic Arabidopsis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6