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Ectopic expression of citrus UDP-GLUCOSYL TRANSFERASE gene enhances anthocyanin and proanthocyanidins contents and confers high light tolerance in Arabidopsis
Qiang Xu
Huazhong Agriculture University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1786-9696
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background Citrus fruits are consumed freshly or as juice to directly provide various dietary flavonoids to humans. Diverse metabolites at different levels are present among Citrus genera and many flavonoids biosynthetic genes were induced after abiotic stresses. To better understand the underlying mechanism, we designed experiments to overexpress a UDP-GLUCOSYL TRANSFERASE gene from Citrus sinensis (sweet orange) to evaluate its possible function in metabolism and response to stress.Results Our results demonstrated that overexpression of Cs-UGT78D3 resulted in high accumulation of proanthocyanidins in the seed coat and a dark brown color to transgenic Arabidopsis seeds. In addition, the total contents of flavonoid and anthocyanin were significantly enhanced in the leaves of overexpressed lines. Gene expression analyses indicated that many flavonoid (flavonol) and anthocyanin genes were up-regulated by 4-15 folds in transgenic Arabidopsis. Moreover, after 14 days of high light stress treatment, the transgenic Arabidopsis lines showed strong antioxidant activity and higher total contents of anthocyanins and flavonoids in leaves compared with the wild type.Conclusion Our study concluded that the citrus Cs-UGT78D3 gene is involved in proanthocyanidins accumulation in seed coats and confers tolerance to high light stress by accumulating the total anthocyanin and flavonoid contents with better antioxidant potential (due to photoprotective activity of anthocyanin) in the transgenic Arabidopsis.
Keywords
Citrus, proanthocyanidins, total flavonoid contents, anthocyanin, photoprotective, Arabidopsis.
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On 02 Dec, 2019
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On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
No comments provided
Review #2 received
Received 30 Oct, 2019
Editorial decision: Major revision
On 30 Oct, 2019
Reviewer #2 agreed
On 16 Oct, 2019
Reviewer #1 agreed
On 15 Oct, 2019
Review #1 received
Received 15 Oct, 2019
Reviewers invited
Invitations sent on 10 Oct, 2019
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On 29 Sep, 2019
Editor assigned
On 24 Sep, 2019
Editor invited
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On 19 Sep, 2019
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Subject Areas
Plant Molecular Biology and Genetics
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Ectopic expression of citrus UDP-GLUCOSYL TRANSFERASE gene enhances anthocyanin and proanthocyanidins contents and confers high light tolerance in Arabidopsis
Qiang Xu
Huazhong Agriculture University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1786-9696
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 5
Posted 20 Dec, 2019
Published
On 30 Dec, 2019
No community comments so far
Editorial decision: Accept
On 19 Dec, 2019
Editor assigned
On 18 Dec, 2019
Submission checks complete
On 17 Dec, 2019
Editor invited
On 17 Dec, 2019
No comments provided
Submission checks complete
On 17 Dec, 2019
Editorial decision: Minor revision
On 17 Dec, 2019
No comments provided
Submission checks complete
On 11 Dec, 2019
Editorial decision: Minor revision
On 10 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 04 Dec, 2019
Review #1 received
Received 03 Dec, 2019
Reviewer #2 agreed
On 03 Dec, 2019
Review #2 received
Received 03 Dec, 2019
Editor assigned
On 02 Dec, 2019
Reviewers invited
Invitations sent on 02 Dec, 2019
Reviewer #1 agreed
On 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
No comments provided
Review #2 received
Received 30 Oct, 2019
Editorial decision: Major revision
On 30 Oct, 2019
Reviewer #2 agreed
On 16 Oct, 2019
Reviewer #1 agreed
On 15 Oct, 2019
Review #1 received
Received 15 Oct, 2019
Reviewers invited
Invitations sent on 10 Oct, 2019
Submission checks complete
On 29 Sep, 2019
Editor assigned
On 24 Sep, 2019
Editor invited
On 23 Sep, 2019
First submitted
On 19 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Molecular Biology and Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background Citrus fruits are consumed freshly or as juice to directly provide various dietary flavonoids to humans. Diverse metabolites at different levels are present among Citrus genera and many flavonoids biosynthetic genes were induced after abiotic stresses. To better understand the underlying mechanism, we designed experiments to overexpress a UDP-GLUCOSYL TRANSFERASE gene from Citrus sinensis (sweet orange) to evaluate its possible function in metabolism and response to stress.Results Our results demonstrated that overexpression of Cs-UGT78D3 resulted in high accumulation of proanthocyanidins in the seed coat and a dark brown color to transgenic Arabidopsis seeds. In addition, the total contents of flavonoid and anthocyanin were significantly enhanced in the leaves of overexpressed lines. Gene expression analyses indicated that many flavonoid (flavonol) and anthocyanin genes were up-regulated by 4-15 folds in transgenic Arabidopsis. Moreover, after 14 days of high light stress treatment, the transgenic Arabidopsis lines showed strong antioxidant activity and higher total contents of anthocyanins and flavonoids in leaves compared with the wild type.Conclusion Our study concluded that the citrus Cs-UGT78D3 gene is involved in proanthocyanidins accumulation in seed coats and confers tolerance to high light stress by accumulating the total anthocyanin and flavonoid contents with better antioxidant potential (due to photoprotective activity of anthocyanin) in the transgenic Arabidopsis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6