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A Novel Experimental Porcine Model to Assess the Impact of Differential Pulmonary Blood Flow on Ischemia-Reperfusion Injury After Unilateral Lung Transplantation
Anna Elisabeth Frick
Katholieke Universiteit Leuven Universitaire Ziekenhuizen Leuven
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2959-2137
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View the published version at Intensive Care Medicine Experimental.
Background Primary graft dysfunction (PGD) remains a major obstacle after lung transplantation. Ischemia-reperfusion injury is a known contributor to the development of PGD following lung transplantation. We developed a novel approach to assess the impact of increased pulmonary blood flow in a large porcine single-left lung transplantation model.
Materials Twelve porcine left lung transplants were divided in two groups (n = 6, in low (LF) and high flow (HF) group). Donor lungs were stored for 24 hours on ice, followed by left lung transplantation. In the HF group, recipient animals were observed for 6h after reperfusion with partially clamping right pulmonary artery PA to achieve a higher flow (target flow 40 – 60% of total cardiac output) to the transplanted lung compared to the LF group, where the right pulmonary artery was not clamped.
Results Survival at 6 hours was 100% in both groups. Histological, functional and biological assessment did not significantly differ between both groups during the first 6 hours of reperfusion. injury was also present in the right native lung and showed signs compatible with the pathophysiological hallmarks of ischemia-reperfusion injury.
Conclusions Partial Clamping native pulmonary artery in large animal lung transplantation setting to study development of ischemia reperfusion is feasible. This allows the study of pulmonary flow as a contributor in the process of ischemia-reperfusion injury. Our findings might impact future studies with extra-corporeal devices and represents a specific intra-operative problem during bilateral sequential single lung transplantation.
Keywords
Porcine left lung transplantation, primary graft dysfunction, pulmonary vascular resistance
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On 17 Jan, 2021
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On 13 Jan, 2021
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On 13 Jan, 2021
Editor invited
On 13 Jan, 2021
Version 2
Posted 30 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 04 Jan, 2021
Reviewer #1 agreed
On 16 Dec, 2020
Review #1 received
Received 16 Dec, 2020
Reviewers invited
Invitations sent on 09 Dec, 2020
Editor assigned
On 08 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
No comments provided
Editorial decision: Major revision
On 15 Sep, 2020
Review #2 received
Received 01 Sep, 2020
Review #3 received
Received 01 Sep, 2020
Review #1 received
Received 30 Aug, 2020
Reviewers invited
Invitations sent on 24 Aug, 2020
Reviewer #1 agreed
On 24 Aug, 2020
Reviewer #2 agreed
On 24 Aug, 2020
Reviewer #3 agreed
On 24 Aug, 2020
Editor assigned
On 23 Aug, 2020
Submission checks complete
On 22 Aug, 2020
Editor invited
On 22 Aug, 2020
First submitted
On 21 Aug, 2020
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This preprint is under consideration at Intensive Care Medicine Experimental. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Articles
A Novel Experimental Porcine Model to Assess the Impact of Differential Pulmonary Blood Flow on Ischemia-Reperfusion Injury After Unilateral Lung Transplantation
Anna Elisabeth Frick
Katholieke Universiteit Leuven Universitaire Ziekenhuizen Leuven
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2959-2137
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Published
On 05 Feb, 2021
No community comments so far
Editorial decision: Accept
On 17 Jan, 2021
Editor assigned
On 13 Jan, 2021
Submission checks complete
On 13 Jan, 2021
Editor invited
On 13 Jan, 2021
Version 2
Posted 30 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 04 Jan, 2021
Reviewer #1 agreed
On 16 Dec, 2020
Review #1 received
Received 16 Dec, 2020
Reviewers invited
Invitations sent on 09 Dec, 2020
Editor assigned
On 08 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
No comments provided
Editorial decision: Major revision
On 15 Sep, 2020
Review #2 received
Received 01 Sep, 2020
Review #3 received
Received 01 Sep, 2020
Review #1 received
Received 30 Aug, 2020
Reviewers invited
Invitations sent on 24 Aug, 2020
Reviewer #1 agreed
On 24 Aug, 2020
Reviewer #2 agreed
On 24 Aug, 2020
Reviewer #3 agreed
On 24 Aug, 2020
Editor assigned
On 23 Aug, 2020
Submission checks complete
On 22 Aug, 2020
Editor invited
On 22 Aug, 2020
First submitted
On 21 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Intensive Care Medicine Experimental.
Background Primary graft dysfunction (PGD) remains a major obstacle after lung transplantation. Ischemia-reperfusion injury is a known contributor to the development of PGD following lung transplantation. We developed a novel approach to assess the impact of increased pulmonary blood flow in a large porcine single-left lung transplantation model.
Materials Twelve porcine left lung transplants were divided in two groups (n = 6, in low (LF) and high flow (HF) group). Donor lungs were stored for 24 hours on ice, followed by left lung transplantation. In the HF group, recipient animals were observed for 6h after reperfusion with partially clamping right pulmonary artery PA to achieve a higher flow (target flow 40 – 60% of total cardiac output) to the transplanted lung compared to the LF group, where the right pulmonary artery was not clamped.
Results Survival at 6 hours was 100% in both groups. Histological, functional and biological assessment did not significantly differ between both groups during the first 6 hours of reperfusion. injury was also present in the right native lung and showed signs compatible with the pathophysiological hallmarks of ischemia-reperfusion injury.
Conclusions Partial Clamping native pulmonary artery in large animal lung transplantation setting to study development of ischemia reperfusion is feasible. This allows the study of pulmonary flow as a contributor in the process of ischemia-reperfusion injury. Our findings might impact future studies with extra-corporeal devices and represents a specific intra-operative problem during bilateral sequential single lung transplantation.
Figure 1
Figure 2
Figure 3