A Novel Experimental Porcine Model to Assess the Impact of Differential Pulmonary Blood Flow on Ischemia-Reperfusion Injury After Unilateral Lung Transplantation
Background Primary graft dysfunction (PGD) remains a major obstacle after lung transplantation. Ischemia-reperfusion injury is a known contributor to the development of PGD following lung transplantation. We developed a novel approach to assess the impact of increased pulmonary blood flow in a large porcine single-left lung transplantation model.
Materials Twelve porcine left lung transplants were divided in two groups (n = 6, in low (LF) and high flow (HF) group). Donor lungs were stored for 24 hours on ice, followed by left lung transplantation. In the HF group, recipient animals were observed for 6h after reperfusion with partially clamping right pulmonary artery PA to achieve a higher flow (target flow 40 – 60% of total cardiac output) to the transplanted lung compared to the LF group, where the right pulmonary artery was not clamped.
Results Survival at 6 hours was 100% in both groups. Histological, functional and biological assessment did not significantly differ between both groups during the first 6 hours of reperfusion. injury was also present in the right native lung and showed signs compatible with the pathophysiological hallmarks of ischemia-reperfusion injury.
Conclusions Partial Clamping native pulmonary artery in large animal lung transplantation setting to study development of ischemia reperfusion is feasible. This allows the study of pulmonary flow as a contributor in the process of ischemia-reperfusion injury. Our findings might impact future studies with extra-corporeal devices and represents a specific intra-operative problem during bilateral sequential single lung transplantation.
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On 05 Feb, 2021
On 17 Jan, 2021
On 13 Jan, 2021
On 13 Jan, 2021
On 13 Jan, 2021
Posted 30 Dec, 2020
On 04 Jan, 2021
On 16 Dec, 2020
Received 16 Dec, 2020
Invitations sent on 09 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 15 Sep, 2020
Received 01 Sep, 2020
Received 01 Sep, 2020
Received 30 Aug, 2020
Invitations sent on 24 Aug, 2020
On 24 Aug, 2020
On 24 Aug, 2020
On 24 Aug, 2020
On 23 Aug, 2020
On 22 Aug, 2020
On 22 Aug, 2020
On 21 Aug, 2020
A Novel Experimental Porcine Model to Assess the Impact of Differential Pulmonary Blood Flow on Ischemia-Reperfusion Injury After Unilateral Lung Transplantation
On 05 Feb, 2021
On 17 Jan, 2021
On 13 Jan, 2021
On 13 Jan, 2021
On 13 Jan, 2021
Posted 30 Dec, 2020
On 04 Jan, 2021
On 16 Dec, 2020
Received 16 Dec, 2020
Invitations sent on 09 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 15 Sep, 2020
Received 01 Sep, 2020
Received 01 Sep, 2020
Received 30 Aug, 2020
Invitations sent on 24 Aug, 2020
On 24 Aug, 2020
On 24 Aug, 2020
On 24 Aug, 2020
On 23 Aug, 2020
On 22 Aug, 2020
On 22 Aug, 2020
On 21 Aug, 2020
Background Primary graft dysfunction (PGD) remains a major obstacle after lung transplantation. Ischemia-reperfusion injury is a known contributor to the development of PGD following lung transplantation. We developed a novel approach to assess the impact of increased pulmonary blood flow in a large porcine single-left lung transplantation model.
Materials Twelve porcine left lung transplants were divided in two groups (n = 6, in low (LF) and high flow (HF) group). Donor lungs were stored for 24 hours on ice, followed by left lung transplantation. In the HF group, recipient animals were observed for 6h after reperfusion with partially clamping right pulmonary artery PA to achieve a higher flow (target flow 40 – 60% of total cardiac output) to the transplanted lung compared to the LF group, where the right pulmonary artery was not clamped.
Results Survival at 6 hours was 100% in both groups. Histological, functional and biological assessment did not significantly differ between both groups during the first 6 hours of reperfusion. injury was also present in the right native lung and showed signs compatible with the pathophysiological hallmarks of ischemia-reperfusion injury.
Conclusions Partial Clamping native pulmonary artery in large animal lung transplantation setting to study development of ischemia reperfusion is feasible. This allows the study of pulmonary flow as a contributor in the process of ischemia-reperfusion injury. Our findings might impact future studies with extra-corporeal devices and represents a specific intra-operative problem during bilateral sequential single lung transplantation.
Figure 1
Figure 2
Figure 3