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Research article
A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze]
Xinghui Li
Nanjing Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6451-7453
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Tea plant (Camellia sinensis) is one of the most popular non-alcoholic beverage worldwide. Lateral roots (LRs) of tea plant are the main organ used for tea plant to absorb soil moisture and nutrients. Lateral roots formation and development are tightly regulated by the nitrogen and auxin signaling pathway. In order to understand the function of auxin and nitrogen signaling in LRs formation and development, transcriptome analysis was applied to investigate the differentially expressed genes involved in lateral roots of tea plants treated with indole-3-butyric acid (IBA), N-1-naphthylphthalamic acid (NPA), low and high nitrogen concentration.
Results A total of 296 common differentially expressed genes were mainly identified and annotated to four signaling pathways, such as nitrogen metabolism, plant hormone signal transduction, Glutathione metabolism and transcription factors. RNA-sequencing results revealed that majority of differentially expressed genes play important roles in nitrogen metabolism and hormonal signal transduction. Low nitrogen condition induced the biosynthesis of auxin and accumulation of transcripts, thereby regulating lateral roots formation. Furthermore, metabolism of cytokinin and ethylene biosynthesis were also involved in lateral roots development. Transcription factors like MYB genes also contributed to the lateral roots formation of tea plants through secondary cell wall biosynthesis. Reversed phase ultra performance liquid chromatography (RP-UPLC) results showed that the auxin concentration in lateral roots was increased, while the nitrogen level decreased. Thus, tea plant lateral roots formation could be induced by low nitrogen concentration via auxin biosynthesis and accumulation.
Conclusion This study provides new insights into the mechanisms associated with nitrogen and auxin signaling pathways to regulate LRs formation and arises new clues for the efficient utilization of nitrogen in tea plant at the genetic level.
Keywords
Nitrogen, Auxin, Lateral roots, Signaling pathway, Camellia sinensis
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Reviewer #1 agreed
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze]
Xinghui Li
Nanjing Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6451-7453
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 4
Posted 26 May, 2020
No community comments so far
Editor assigned
On 14 May, 2020
Submission checks complete
On 13 May, 2020
Editor invited
On 13 May, 2020
No comments provided
Editorial decision: Minor revision
On 10 May, 2020
Editor assigned
On 09 Mar, 2020
Editor invited
On 08 Mar, 2020
Submission checks complete
On 05 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 01 Mar, 2020
Review #2 received
Received 23 Feb, 2020
Review #1 received
Received 07 Feb, 2020
Reviewer #2 agreed
On 06 Feb, 2020
Reviewers invited
Invitations sent on 03 Feb, 2020
Reviewer #1 agreed
On 03 Feb, 2020
Editor assigned
On 23 Jan, 2020
Submission checks complete
On 22 Jan, 2020
Editor invited
On 22 Jan, 2020
No comments provided
Editorial decision: Major revision
On 21 Nov, 2019
Review #2 received
Received 10 Nov, 2019
Reviewer #2 agreed
On 28 Oct, 2019
Review #1 received
Received 25 Oct, 2019
Reviewers invited
Invitations sent on 14 Oct, 2019
Reviewer #1 agreed
On 14 Oct, 2019
Submission checks complete
On 10 Oct, 2019
Editor assigned
On 07 Oct, 2019
Editor invited
On 06 Oct, 2019
First submitted
On 03 Oct, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background Tea plant (Camellia sinensis) is one of the most popular non-alcoholic beverage worldwide. Lateral roots (LRs) of tea plant are the main organ used for tea plant to absorb soil moisture and nutrients. Lateral roots formation and development are tightly regulated by the nitrogen and auxin signaling pathway. In order to understand the function of auxin and nitrogen signaling in LRs formation and development, transcriptome analysis was applied to investigate the differentially expressed genes involved in lateral roots of tea plants treated with indole-3-butyric acid (IBA), N-1-naphthylphthalamic acid (NPA), low and high nitrogen concentration.
Results A total of 296 common differentially expressed genes were mainly identified and annotated to four signaling pathways, such as nitrogen metabolism, plant hormone signal transduction, Glutathione metabolism and transcription factors. RNA-sequencing results revealed that majority of differentially expressed genes play important roles in nitrogen metabolism and hormonal signal transduction. Low nitrogen condition induced the biosynthesis of auxin and accumulation of transcripts, thereby regulating lateral roots formation. Furthermore, metabolism of cytokinin and ethylene biosynthesis were also involved in lateral roots development. Transcription factors like MYB genes also contributed to the lateral roots formation of tea plants through secondary cell wall biosynthesis. Reversed phase ultra performance liquid chromatography (RP-UPLC) results showed that the auxin concentration in lateral roots was increased, while the nitrogen level decreased. Thus, tea plant lateral roots formation could be induced by low nitrogen concentration via auxin biosynthesis and accumulation.
Conclusion This study provides new insights into the mechanisms associated with nitrogen and auxin signaling pathways to regulate LRs formation and arises new clues for the efficient utilization of nitrogen in tea plant at the genetic level.
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