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Research article
Evaluating Common Variants in NOS1AP in Patients with Implantable Cardioverter Defibrillator for Secondary Prevention
Gao Chuanyu
People's Hospital of Zhengzhou University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5817-5212
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Contemporary researchers found single nucleotide polymorphisms (SNPs) in nitric oxide synthase 1 adaptor protein (NOS1AP) gene are associated with altered QT intervals and SCD. However, the clinical utility and implications of these SNP have not been described. This study aims to explore the clinical utility and implications of SNPs in nitric oxide synthase 1 adaptor protein (NOS1AP) in patients with implantable cardioverter defibrillator (ICD) for secondary prevention.
Methods
We firstly conducted a case-control study to evaluate the associations between hot-spot SNPs in NOS1AP (rs12143842, rs10494366, rs12567209 and rs16847548) and patients with ICD for secondary prevention. Then the clinical values of the positive SNPs were further evaluated in these patients. All patients were divided into three groups according to different genotypes of the positive SNPs. ICD interrogation data at 3, 12 months, and 3 years after implantation, which include rapid ventricular arrhythmia episodes and appropriate therapies, were analyzed in three genotypes.
Results
Case-control study revealed significant allelic association between rs10494366 and ICD recipients who experienced appropriate therapies. After a mean follow-up time of 31.70 ± 9.15 months, we detected not only significant difference among three genotypes on the distribution of ICD shocks and appropriate therapies, but apparently also the correlation of rs10494366 and ICD shocks. Furthermore, under kaplan-meier and cox regression analysis, TT genotype both showed higher risk for sudden cardiac death (SCD) compared with GG genotype.
Conclusions
The present study revealed that SNP rs10494366 was associated with appropriate therapies and SCD in patients with ICD for secondary prevention for the first time.
Keywords
Nitric oxide synthase 1 adaptor protein gene, Implantable cardioverter defibrillator, QT interval, Sudden cardiac death, Single nucleotide polymorphism
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.docx
Additional file 1: The primer sequences of four SNPs in NOS1AP.
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CURRENT STATUS: Under Review
Version 1
Posted 22 Sep, 2020
No community comments so far
Review #1 received
Received 02 Dec, 2020
Reviewer #1 agreed
On 10 Nov, 2020
Reviewers invited
Invitations sent on 14 Oct, 2020
Editor invited
On 17 Sep, 2020
Editor assigned
On 15 Sep, 2020
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On 14 Sep, 2020
First submitted
On 13 Sep, 2020
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Subject Areas
Cardiac & Cardiovascular Systems
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This preprint is under consideration at BMC Cardiovascular Disorders. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Evaluating Common Variants in NOS1AP in Patients with Implantable Cardioverter Defibrillator for Secondary Prevention
Gao Chuanyu
People's Hospital of Zhengzhou University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5817-5212
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Sep, 2020
No community comments so far
Review #1 received
Received 02 Dec, 2020
Reviewer #1 agreed
On 10 Nov, 2020
Reviewers invited
Invitations sent on 14 Oct, 2020
Editor invited
On 17 Sep, 2020
Editor assigned
On 15 Sep, 2020
Submission checks complete
On 14 Sep, 2020
First submitted
On 13 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cardiac & Cardiovascular Systems
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Contemporary researchers found single nucleotide polymorphisms (SNPs) in nitric oxide synthase 1 adaptor protein (NOS1AP) gene are associated with altered QT intervals and SCD. However, the clinical utility and implications of these SNP have not been described. This study aims to explore the clinical utility and implications of SNPs in nitric oxide synthase 1 adaptor protein (NOS1AP) in patients with implantable cardioverter defibrillator (ICD) for secondary prevention.
Methods
We firstly conducted a case-control study to evaluate the associations between hot-spot SNPs in NOS1AP (rs12143842, rs10494366, rs12567209 and rs16847548) and patients with ICD for secondary prevention. Then the clinical values of the positive SNPs were further evaluated in these patients. All patients were divided into three groups according to different genotypes of the positive SNPs. ICD interrogation data at 3, 12 months, and 3 years after implantation, which include rapid ventricular arrhythmia episodes and appropriate therapies, were analyzed in three genotypes.
Results
Case-control study revealed significant allelic association between rs10494366 and ICD recipients who experienced appropriate therapies. After a mean follow-up time of 31.70 ± 9.15 months, we detected not only significant difference among three genotypes on the distribution of ICD shocks and appropriate therapies, but apparently also the correlation of rs10494366 and ICD shocks. Furthermore, under kaplan-meier and cox regression analysis, TT genotype both showed higher risk for sudden cardiac death (SCD) compared with GG genotype.
Conclusions
The present study revealed that SNP rs10494366 was associated with appropriate therapies and SCD in patients with ICD for secondary prevention for the first time.
Figure 1
Figure 2