See the published version of this article at BMC Women's Health.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women
Dong Hang
Nanjing Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Women's Health.
Background Although human papillomavirus (HPV) infection has been recognized as the cause of cervical cancer in over 99% of cases, only a minority of HPV-infected women develop this malignancy. Emerging evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains unknown.
Methods In this case-control study, we included 591 cervical cancer cases and 373 cancer-free controls, all of whom were infected with high-risk HPV. Relative mtCN in cervical cancer exfoliated cells was measured by qRT-PCR assays, and logistic regression analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs). Interaction between mtCN and HPV types was assessed by using the Wald test in logistic regression models.
Results HPV16, 18, 52, and 58 were the most common types in both case and control groups. Median mtCN in cases was significantly higher than that in controls (1.63 vs. 1.23, P = 0.03). After adjustment for age and HPV types, the highest quartile of mtCN was associated with increased odds of having cervical cancer (OR = 1.77, 95% CI = 1.19, 2.62; P < 0.01), as compared to the lowest quartile. A dose-response effect of mtCN on cervical cancer was also observed (Ptrend < 0.001). The interaction between mtCN and HPV types was statistically nonsignificant.
Conclusions In women who test HPV positive, the increase of mtCN in cervical exfoliated cells is associated with cervical cancer. This suggests a potential role of mtCN in cervical carcinogenesis.
Keywords
mitochondrial DNA copy number; mitochondrion; cervical cancer; human papillomavirus; case-control study
Figure 1
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 5
Posted 18 Jun, 2020
Published
On 02 Jul, 2020
No community comments so far
Submission checks complete
On 17 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 15 Jun, 2020
Editor assigned
On 25 May, 2020
Submission checks complete
On 24 May, 2020
Editor invited
On 24 May, 2020
No comments provided
Editorial decision: Major revision
On 21 May, 2020
Reviewer #1 agreed
On 21 Feb, 2020
Reviewers invited
Invitations sent on 05 Feb, 2020
Editor assigned
On 28 Jan, 2020
Submission checks complete
On 27 Jan, 2020
Editor invited
On 27 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 19 Jan, 2020
Review #2 received
Received 18 Jan, 2020
Reviewer #2 agreed
On 08 Jan, 2020
Review #1 received
Received 07 Jan, 2020
Reviewer #1 agreed
On 24 Dec, 2019
Editor assigned
On 23 Dec, 2019
Reviewers invited
Invitations sent on 23 Dec, 2019
Submission checks complete
On 22 Dec, 2019
Editor invited
On 22 Dec, 2019
No comments provided
Review #2 received
Received 25 Nov, 2019
Editorial decision: Major revision
On 25 Nov, 2019
Review #1 received
Received 23 Nov, 2019
Reviewer #2 agreed
On 08 Nov, 2019
Reviewer #1 agreed
On 04 Nov, 2019
Reviewers invited
Invitations sent on 30 Oct, 2019
Editor assigned
On 28 Oct, 2019
Editor invited
On 25 Oct, 2019
Submission checks complete
On 24 Oct, 2019
First submitted
On 20 Oct, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Preventive Medicine
Learn more about our company.
This preprint is under consideration at BMC Women's Health. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women
Dong Hang
Nanjing Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 5
Posted 18 Jun, 2020
Published
On 02 Jul, 2020
No community comments so far
Submission checks complete
On 17 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 15 Jun, 2020
Editor assigned
On 25 May, 2020
Submission checks complete
On 24 May, 2020
Editor invited
On 24 May, 2020
No comments provided
Editorial decision: Major revision
On 21 May, 2020
Reviewer #1 agreed
On 21 Feb, 2020
Reviewers invited
Invitations sent on 05 Feb, 2020
Editor assigned
On 28 Jan, 2020
Submission checks complete
On 27 Jan, 2020
Editor invited
On 27 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 19 Jan, 2020
Review #2 received
Received 18 Jan, 2020
Reviewer #2 agreed
On 08 Jan, 2020
Review #1 received
Received 07 Jan, 2020
Reviewer #1 agreed
On 24 Dec, 2019
Editor assigned
On 23 Dec, 2019
Reviewers invited
Invitations sent on 23 Dec, 2019
Submission checks complete
On 22 Dec, 2019
Editor invited
On 22 Dec, 2019
No comments provided
Review #2 received
Received 25 Nov, 2019
Editorial decision: Major revision
On 25 Nov, 2019
Review #1 received
Received 23 Nov, 2019
Reviewer #2 agreed
On 08 Nov, 2019
Reviewer #1 agreed
On 04 Nov, 2019
Reviewers invited
Invitations sent on 30 Oct, 2019
Editor assigned
On 28 Oct, 2019
Editor invited
On 25 Oct, 2019
Submission checks complete
On 24 Oct, 2019
First submitted
On 20 Oct, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Preventive Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Women's Health.
Background Although human papillomavirus (HPV) infection has been recognized as the cause of cervical cancer in over 99% of cases, only a minority of HPV-infected women develop this malignancy. Emerging evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains unknown.
Methods In this case-control study, we included 591 cervical cancer cases and 373 cancer-free controls, all of whom were infected with high-risk HPV. Relative mtCN in cervical cancer exfoliated cells was measured by qRT-PCR assays, and logistic regression analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs). Interaction between mtCN and HPV types was assessed by using the Wald test in logistic regression models.
Results HPV16, 18, 52, and 58 were the most common types in both case and control groups. Median mtCN in cases was significantly higher than that in controls (1.63 vs. 1.23, P = 0.03). After adjustment for age and HPV types, the highest quartile of mtCN was associated with increased odds of having cervical cancer (OR = 1.77, 95% CI = 1.19, 2.62; P < 0.01), as compared to the lowest quartile. A dose-response effect of mtCN on cervical cancer was also observed (Ptrend < 0.001). The interaction between mtCN and HPV types was statistically nonsignificant.
Conclusions In women who test HPV positive, the increase of mtCN in cervical exfoliated cells is associated with cervical cancer. This suggests a potential role of mtCN in cervical carcinogenesis.
Figure 1