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Research article
Pio Zeppa
2. University of Salerno, Department of Medicine and Surgery “Scuola Medica Salernitana”, Salerno, Italy
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7358-5925
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: to assess whether immunohistochemical (IHC) algorithms used to classify the cell of origin (COO) of nodal Diffuse Large B-cell lymphoma (nDLBCL) in Germinal Center type (GCB) and non-GCB subtypes may be applied to Primary Cutaneous B-cell lymphoma (PCBCL) too, and which of these algorithms performs better on PCBCL.
Design: retrospective case control study.
Setting: Pathology Department of the University Hospital “San Giovanni di Dio e Ruggi d’Aragona” Salerno, Italy.
Participants : fourteen PCBCL, including Primary Cutaneous follicle centre lymphoma (PCFCL) and primary cutaneous diffuse large B-cell lymphoma, Leg type (PCDLBCL-LT) and 14 nDLBCL were evaluated for 7-year period (January 2011 to December 2017). Primary cutaneous marginal zone cell lymphoma (PCMZL) cases were not included in the present study.
Intervention: evaluation of immunohistochemical CD10, BCL6, MUM1/IRF4, BCL2, MYC and Ki-67 expression and classification according to three different algorithms. Gene expression profiling (GEP) was performed on the same series using Lymph2Cx assay (Nanostring). The data obtained were compared and analysed.
Results: all the IHC algorithms showed 13 GCB and 15 non-GCB. GEP showed 12 GCB, 12 activated B cell–type and 4 unclassified.
Conclusions: the PCBCL were classifiable as GCB and non-GCB like the nDLBCL as IHC algorithms were concordant to GEP and produced the same results.
Keywords
Primary cutaneous B-cell lymphoma, nodal diffuse large B-cell lymphoma, Cell of Origin, Gene expression profiling
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This is a preprint. It has not completed peer review.
Research article
Pio Zeppa
2. University of Salerno, Department of Medicine and Surgery “Scuola Medica Salernitana”, Salerno, Italy
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7358-5925
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: to assess whether immunohistochemical (IHC) algorithms used to classify the cell of origin (COO) of nodal Diffuse Large B-cell lymphoma (nDLBCL) in Germinal Center type (GCB) and non-GCB subtypes may be applied to Primary Cutaneous B-cell lymphoma (PCBCL) too, and which of these algorithms performs better on PCBCL.
Design: retrospective case control study.
Setting: Pathology Department of the University Hospital “San Giovanni di Dio e Ruggi d’Aragona” Salerno, Italy.
Participants : fourteen PCBCL, including Primary Cutaneous follicle centre lymphoma (PCFCL) and primary cutaneous diffuse large B-cell lymphoma, Leg type (PCDLBCL-LT) and 14 nDLBCL were evaluated for 7-year period (January 2011 to December 2017). Primary cutaneous marginal zone cell lymphoma (PCMZL) cases were not included in the present study.
Intervention: evaluation of immunohistochemical CD10, BCL6, MUM1/IRF4, BCL2, MYC and Ki-67 expression and classification according to three different algorithms. Gene expression profiling (GEP) was performed on the same series using Lymph2Cx assay (Nanostring). The data obtained were compared and analysed.
Results: all the IHC algorithms showed 13 GCB and 15 non-GCB. GEP showed 12 GCB, 12 activated B cell–type and 4 unclassified.
Conclusions: the PCBCL were classifiable as GCB and non-GCB like the nDLBCL as IHC algorithms were concordant to GEP and produced the same results.
Figure 1
Figure 2
Figure 3
Figure 4
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