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Research article
A Proteomic and Transcriptomic Analysis Reveals That hsa_circ_0004182 Promotes the Expression of Glycerol-3-phosphate Dehydrogenase 1 Like via Targeting mir-155 in Coronary Artery Disease
Enzhi Jia
Department of Cardiovascular Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1354-9855
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Coronary artery disease (CAD) is one of the leading causes of mortality in the world, and its early diagnosis remains a huge challenge in clinical medicine. Genetic and environmental factors both contribute to the occurrence and development of CAD. It is reported that the inhibition of mitophagy plays an important role in CAD, while the molecular mechanism is still uncertain. In our previous studies, circulating mir-155 in plasma showed a significant differential expression in CAD populations and controls.
Method: In this study, we detected the expression of RNAs and proteins in human coronary artery samples with genome sequencing. Bioinformatics analysis was conducted to find the binding sites of circRNAs, mir-155 and protein coding genes.
Result: 2135 protein groups, 21703 unique peptides and 1640 circRNAs were identified in the samples. 174 differential expressed proteins and 66 differential expressed circRNAs were screened according to the pathological grading and staging of the samples. GO and KEGG analysis showed that the SDEPs were significantly associated with metabolism, oxidative respiration, and mitochondrial function. Based on the gene-gene interaction and gene-protein interaction analysis, a novel ceRNA network may play a regulatory role in CAD.
Conclusion: Hsa_circ_0004182 may inhibit the expression of mir-155 by acting as miRNA sponges, and repress the post-transcriptional repression of GPD1L mediated by mir-155, and promote GPD1L expression in coronary artery disease.
Keywords
coronary artery disease, ceRNA, proteomic and transcriptomic analysis
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This is a preprint. It has not completed peer review.
Research article
A Proteomic and Transcriptomic Analysis Reveals That hsa_circ_0004182 Promotes the Expression of Glycerol-3-phosphate Dehydrogenase 1 Like via Targeting mir-155 in Coronary Artery Disease
Enzhi Jia
Department of Cardiovascular Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1354-9855
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Coronary artery disease (CAD) is one of the leading causes of mortality in the world, and its early diagnosis remains a huge challenge in clinical medicine. Genetic and environmental factors both contribute to the occurrence and development of CAD. It is reported that the inhibition of mitophagy plays an important role in CAD, while the molecular mechanism is still uncertain. In our previous studies, circulating mir-155 in plasma showed a significant differential expression in CAD populations and controls.
Method: In this study, we detected the expression of RNAs and proteins in human coronary artery samples with genome sequencing. Bioinformatics analysis was conducted to find the binding sites of circRNAs, mir-155 and protein coding genes.
Result: 2135 protein groups, 21703 unique peptides and 1640 circRNAs were identified in the samples. 174 differential expressed proteins and 66 differential expressed circRNAs were screened according to the pathological grading and staging of the samples. GO and KEGG analysis showed that the SDEPs were significantly associated with metabolism, oxidative respiration, and mitochondrial function. Based on the gene-gene interaction and gene-protein interaction analysis, a novel ceRNA network may play a regulatory role in CAD.
Conclusion: Hsa_circ_0004182 may inhibit the expression of mir-155 by acting as miRNA sponges, and repress the post-transcriptional repression of GPD1L mediated by mir-155, and promote GPD1L expression in coronary artery disease.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5