Combination of Artesunate and WNT974 Induces KRAS Protein Degradation by Upregulating E3 Ligase ANACP2 and β-TrCP in the Ubiquitin–Proteasome Pathway

doi:10.21203/rs.3.rs-744967/v2

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Combination of Artesunate and WNT974 Induces KRAS Protein Degradation by Upregulating E3 Ligase ANACP2 and β-TrCP in the Ubiquitin–Proteasome Pathway

https://doi.org/10.21203/rs.3.rs-744967/v2

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published 19 Mar, 2022

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Background

KRAS mutation is one of the dominant gene mutations in colorectal cancer (CRC). Up to present, targeting KRAS for CRC treatment remains a clinical challenge. WNT974 (LGK974) is a porcupine inhibitor that interferes Wnt signaling pathway. Artesunate (ART) is a water-soluble semi-synthetic derivative of artemisinin.

Methods

The synergistic effect of ART and WNT974 combination in reducing CRC cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RT-PCR was utilized for the mRNA levels of KRAS, CUL7, ANAPC2, UBE2M, RNF123, SYVN1, or β-TrCP. Western blot assay was utilized for the protein levels of KRAS, ANAPC2, β-TrCP, or GSK-3β. Xenograft mouse model assay was performed for the anti-CRC effect of combination of ART and WNT974 in vivo. IHC assay was utilized for the levels of KRAS, β-TrCP, or GSK-3β in tumor tissues.

Results

Our study shows that the combination of WNT974 and ART exhibits synergistic effect in reducing CRC growth. The combination treatment significantly reduces KRAS protein level and activity in CRC cells. Interestingly, the combination treatment increases E3 ligases ANAPC2 expression. Our data show that overexpression of ANAPC2 significantly reduces KRAS protein levels, which is reversed by MG132. Knockdown of ANAPC2 in CRC abolishes the combination treatment-reduce KRAS expression. Besides, the treatment also increases the expressions of GSK-3β and E3 ligase β-TrCP that is known to degrade GSK-3β-phosphorylated KRAS protein. Knockdown of β-TrCP- and inhibition of GSK-3β abolish the combination treatment-induce KRAS ubiquitination and reduction in expression.

Conclusions

Our data clearly show that the combination treatment significantly enhances KRAS protein degradation via the ubiquitination ubiquitin–proteasome pathway, which is also demonstrated in xenograft mouse model. The study provides strong scientific evidence for the development of the combination of WNT974 and ART as KRAS-targeting therapeutics for CRC treatment.

Cell Communication and Signaling

Colorectal cancer

KRAS

Artesunate

WNT974

Combination treatment

Protein degradation.

Colorectal cancer (CRC) is one of the most common malignant tumors of the gastrointestinal tract. It is a highly heterogeneous disease with diverse genetic background. KRAS (kirsten rat sarcoma viral oncogene) mutation is one of the dominant mutations in CRC, which accounts for 40% of all the CRC cases [1]. The most common KRAS mutations are at codon 12 and 13, followed by mutations at codons 61 and 146 [2].

KRAS is downstream of epidermal growth factor receptor (EGFR). Upon EGFR activation, the tyrosine kinase in the intracellular region phosphorylates and activates KRAS and hence activates the RAS-RAF-MAPK signaling pathway. The activated KRAS-GTP will then be hydrolyzed by GTPase and switches back to the inactivate KRAS-GDP state. Therefore, KRAS is switched between the active (KRAS-GTP) and inactive (KRAS-GDP) states. However, mutations in KRAS result in aberrant activation of the downstream RAS-RAF-MAPK or phosphoinositide 3-kinase pathways, regardless of the EGFR activation status [3, 4].

Since KRAS activity is regulated by farnesylation-mediated protein modifications [5], farnesyl transferase inhibitors have been developed. Although they were effective in preclinical models, they failed in the clinical studies [6, 7]. Therapeutic approaches also include targeting KRAS downstream signaling with kinase inhibitors for the rapidly accelerated fibrosarcoma, mitogen-activated protein kinase kinase and extracellular signal-regulated kinase. However, only a minority of these provides marginal survival advantages to the CRC patients carrying KRAS mutations; moreover, the treatments result in significant adverse events [8]. Furthermore, CRC patients harboring KRAS mutations are insensitive to the current anti-EGFR therapy [9], which further limits their treatment choices.

Up to present, no effective pharmacological inhibitors for the KRAS oncoproteins has been approved for cancer treatment, leading to the perception that KRAS proteins are 'undruggable'. Indeed, based on the structure and regulatory mechanisms of the KRAS, researchers have identified a number of challenges in targeting KRAS for disease treatments such as lack of binding sites on the KRAS protein surface for the binding of small molecule inhibitors [10–12]. Nevertheless, interrupting the KRAS-membrane interaction and disrupting the KRAS subcellular localization remain as attractive therapeutic strategies. Recent studies on the functionally relevant post-translational modifications of KRAS protein such as phosphorylation and ubiquitylation also suggest new opportunities to inhibit KRAS activity.

WNT974 (also known as LGK974) is a selective and orally bioavailable (PORCN) inhibitor. PORCN is a membrane-bound O-acyltransferase in the endoplasmic reticulum that adds the palmitoyl group to Wnt ligands, which is a necessary step for processing Wnt ligand secretion [13]. WNT974 inhibits PORCN and hence the secretion of Wnt ligands and interferes the Wnt-mediated signaling. WNT974 is developed to treat Wnt-driven cancers [14]. It shows significant effects in several cancer types including ovarian cancer [15], lung cancer [16], squamous cell carcinoma [17, 18], glioblastoma [19, 20] and colon cancer [21]. Indeed, WNT974 is in clinical trial phase I and phase II (NCT01351103, NCT02278133) for CRC treatment [22, 23].

Many bioactive compounds are identified from medicinal herbs [24]. Artemisia annua L. is a medicinal plant used to treat fever and chills. Artemisinin, is an bioactive compound isolated from Artemisia annua L. Artesunate (ART) is a water-soluble semi-synthetic derivative of artemisinin, which is approved for treating severe malaria cases [25]. ART is also known to specifically inhibits cancer cell growth but not the normal cells [26–29]. National Cancer Institute has also conducted a study and found that several cancer cells including CRC are sensitive to ART treatments. ART is now under clinical trials for treating patients with high-grade anal intraepithelial neoplasia and solid tumour [30].

In our study, we found that the combination of WNT974 and ART exhibited synergistic effect in reducing CRC growth. Interestingly, the combination treatment significantly enhanced KRAS protein degradation that may underlie the synergistic effect of the combination treatment.

Reagents

Artesunate (ART) was provided from Kuming Pharmaceutical Co. Ltd. WNT974 was purchased from MedChemExpress company, Dulbecco's Modified Eagle's Medium, fetal bovine serum were purchased from Thermofisher Scientific Company. Antibodies against β-TrCP, GSK-3β, KRAS, ANAPC2 GSK-3βand β-Actin were purchased form Cell Signaling Technology (Danvers, MA). Mouse anti-rabbit IgG-HRP secondary antibody was purchased from San Cruz Biotechnology (Santa Cruz, United States of America).

Cell lines and culture

HCT116, HT29, SW480, SW620, COLO325, COLO205, HCT15 and RKO cells were purchased from American Type Culture Collection (Manassas, USA). Cells were cultured in DMEM supplemented with 10% FBS in a humidified atmosphere containing 5% CO₂ and 95% air at 37°C. The medium was changed every three days, and cells were passaged using 0.05% trypsin/EDTA.

Western blot analysis

Proteins were extracted from CRC cells with RIPA lysis buffer, followed by centrifugation at 13,500 rpm for 15 min at 4°C. Protein concentration was measured using Pierce (R) BCA Protein Assay Kit, and equal amount of protein was separated on 10% SDS-PAGE and transferred to PVDF membranes. After blocking (5% skim milk powder in TBST, 20) for 1 h at room temperature, the membrane was then incubated with the respective primary antibody overnight at 4°C. Afterward, the membrane was incubated with secondary antibody for 1 h at room temperature. All antibodies were diluted in TBS-Tween 20 containing 5% dry milk. The immune-reactive proteins were detected by enhanced chemiluminescence (ECL) using X-ray film and ECL reagent.

KRAS activity

The ubiquitinated proteins were isolated using the UBIQAPTURE-Q® kit (UW8995, Enzo Life Sciences). KRAS protein activity was examined by the KRAS activation assay kit (ab211159, Abcam) following company’s instruction.

Real time PCR analysis

RNA was extracted from CRC cells using TRIzol reagent (Invitrogen), and cDNAs were subsequently prepared by reverse transcription. RT Profiler PCR Array experiment was performed following the instruction of RT2 Profiler PCR Array kit (QIAGEN). Quantitative polymerase chain reaction (PCR) was performed using the Quantitect SYBR Green PCR Master Mix (Qiagen, Valencia, CA) with 1 µL cDNA in a final volume of 10 µL and the following primers at a final concentration of 1000 nM. Primers for ANAPC2 were 5′-GGCAGCAAGGACCTCTTCAT-3’ (forward) and 5′-CTTGCTCAGTTCCTCCAGGG-3’ (reverse). Primers for CUL7 were 5′-GTGGCATTGATACGCGCATT-3’ (forward) and 5′- CTCCAGTCGTGGCTTCTGTT − 3’ (reverse). Primers for UBE2M were 5′-GCAGCAGAAGAAGGAGGAGG-3’ (forward) and 5′-GTAGGTGGAGCCGATGTAGC-3’ (reverse). Primers for RNF123 were 5′-ATCCAGGGTCACAGGCATTG-3’ (forward) and 5′-CCACGCCTTGCCATAATTCG-3’ (reverse). Primers for SYVN1 were 5′-CTGCCTCCTTTTCCTCCAGG-3’ (forward) and 5′-TCTGAGCTAGGGATGCTGGT-3’ (reverse). Primers for NEDD4 were 5′- AGCCAGAGTTCTGCAGGCCCT-3’ (forward) and 5′- GCTGGGAAGTCCGGCATGCA-3’ (reverse). Primers for β-Trcp were 5′- AAGCGAATTCTCACAGGCCA − 3’ (forward) and 5′- TCCATCATAGGCCCCACTGA − 3’ (reverse). Primers for PEAK were 5′- ACCAGTCTCGCCTTGCCCCA − 3’ (forward) and 5′- GGGGAGCGGAATGGGATGCG − 3’ (reverse). Primers for elF5A were 5′- CCTGGTGGGGGAGAAGGGGG-3’ (forward) and 5′-CCTGAGGAGGGGGCAGGTCC-3’ (reverse). Primers for RAK were 5′- TCCCAGCTCCATTTGATTTGTC-3’ (forward) and 5′- TGACCAGATCCCAATCGCTTC-3’ (reverse). Primers for SMURF-2 were 5′- GTGGTTGATGGATCTGGGCA-3’ (forward) and 5′- ACTGTCCACATGTTGCACCA-3’ (reverse). Primers for UBCH5 were 5′- AGCGCATATCAAGGTGGAGT-3’ (forward) and 5′- AGCTGAAGATGCAGATGTCCA − 3’ (reverse). Amplification of the cDNAs was performed using the LightCycler 2000 instrument (Roche, Indianapolis, IN). The cycling conditions comprised a denaturation step for 15 minutes at 95°C, followed by 40 cycles of denaturation (95°C for 15 seconds), annealing (59°C for 20 seconds), and extension (72°C for 15 seconds). After amplification, a melting curve analysis was performed with denaturation at 95°C for 5 seconds, then continuous fluorescence measurement was made from 70°C to 95°C at 0.1°C/second. Each sample was amplified in duplicate.

siRNA transfection

HCT116 cells or SW620 cells were seeded in 6-well plates. p21 or p27 siRNA were diluted to 10µM working concentration for transfection. Then, 5µL Lipofectamine® RNAiMax transfection reagent (Invitrogen) was added to 150µL medium without serum in one tube, and 3µL of prepared siRNA was added to 150µL medium without serum, and incubated for 20 mins at room temperature before adding to the well and cultured for 24h.

CRC-bearing xenograft mouse model

The six-week-old male BALB/c nude mice were housed in the Laboratory Animal House of Hong Kong Baptist University. The animal house is temperature-controlled with a 12 h light/dark cycle. Food and water were available ad libitum. Mice were adapted to the environment for one week before the study. The procedures of all in vivo studies have granted ethics approval by the Animal Experimentation Ethics Committee of the Hong Kong Baptist University. The mice were subcutaneously inoculated with 1 × 10⁶ HCT116 cells in the left armpit. Once tumors were palpable (~ 100 mm³), the tumor-bearing nude mice were randomly divided into groups with five mice in each group for further studies. (1) Vehicle group (daily i.p. saline), (2) ART group (daily i.p. 30mg/kg of artesunate), (3) WNT974 group (daily i.p. 5mg/kg of WNT974), (4) ART combined with WNT974 group (daily i.p. 30mg/kg artesunate and 5mg/kg WNT974), (5) 5-Fu group (daily i.p. 10mg/kg of 5-Fu). The tumor size and body weight were monitored every day.

Immunohistochemical staining

Paraffin sections of tumors were deparaffinized using xylene and then rehydrated by immersing in alcohol at series of concentrations. Endogenous peroxidase was quenched by 10 min incubation with 3% hydrogen peroxide. Blocking serum of 1:10 was used for blocking the non-specific bindings of epitopes. The sections were incubated with β-TrCP, GSK-3β and KRAS antibody of 1:100 at 4°C overnight. Slides were then washed three times, incubated with a biotinylated secondary antibody (Santa Cruz) for 30 min, and then with peroxidase substrate for 10 min. The sections were finally washed, incubated in deionized water for 5 min, counterstained with hematoxylin before analyzing by microscopy (NIKON Eclipse ci, NIKON digital sight DS-FI2, Japan).

Statistical analysis

The data were shown as mean ± standard errors with three independent experiments. Statistical analysis was carried out with GraphPad Prism 8.0 software. Data are taken as significance when p༜0.05.

The combination of WNT974 and artesunate (ART) reduces KRAS protein level and activity in CRC cells

The combination of WNT974 and ART exhibited synergistic effect in reducing CRC cell viability (Fig. 1A) as indicated by the combination index (CI) < 1. Interestingly, the combination of WNT974 and ART significantly reduced KRAS protein levels (Fig. 1B) in the CRC cells when compared to WNT974 or ART mono-treatments. The treatment did not affect the KRAS mRNA levels (Fig. 1C). We also examined the KRAS activity in these cells by using Raf1 RBD agarose beads to selectively pull-down the active form of KRAS from the protein samples. Figure 1B showed that the combination treatment significantly reduced KRAS activity in the CRC cells when compared to control or the monotreatments. These results imply that the reduced KRAS protein expression and activity may be associated with the synergistic effect of the combination treatment in reducing CRC growth.

The combination of WNT974 and ART induces KRAS protein degradation in CRC cells

Post-translational modifications of KRAS protein such as ubiquitylation and degradation may reduce the protein expression [31]. The ubiquitin proteasome pathway consists of concerted actions of enzymes that link chains of the polypeptide co-factor, ubiquitin, onto proteins to mark them for degradation [32]. We next examined whether the combination treatment affected KRAS ubiquitination. As shown in Fig. 2A, the combination treatment markedly enhanced KARS ubiquitination in the CRC cells when compared to the mono-treatments. To further examine whether the treatment affected KRAS protein degradation, we treated the cells with WNT974, ART or the combination of both in presence or absence of MG132. MG132 is a specific, potent, reversible cell-permeable proteasome inhibitor [33]. As shown in Fig. 2B and 2C, MG132 abolished the combination treatment-reduced KRAS protein expression, suggesting the combination of WNT974 and ART induces KRAS degradation via the ubiquitination proteasome pathway.

The combination of WNT974 and ART induces KRAS protein degradation by increasing anaphase promoting complex subunit 2 (ANAPC2) expression

Next, we examined how the combination treatment induced KRAS degradation in CRC cells. Firstly, we used PCR array for the human ubiquitination pathway (genes layout in Table 1) to examine whether the treatments affected the expressions of the genes that are involved in the ubiquitination pathway. As shown in Fig. 3A, the heat map indicated that the treatments affected the gene expressions, the numbers of up- or down-regulated genes were shown in Fig. 3B. The results showed that, compared to control, ART treatment upregulated 15 genes and downregulated 34 genes; WNT974 treatment upregulated 13 genes and downregulated 20 genes; and the combination treatment upregulated 18 genes and downregulated 27 genes. Compared to ART treatment, the combination treatment upregulated 12 genes and downregulated 9 genes. Compared to WNT974 treatment, the combination treatment upregulated 12 genes and downregulated 10 genes. More importantly, we found that 5 genes were upregulated by more than 2-fold under the combination treatment when compared to the mono-treatments. These ubiquitination related genes were CUL7 (cullin), ANAPC2, UBE2M (ubiquitin conjugating enzyme E2M), SYVN1 (synoviolin 1) and RNF123 (RING finger protein123) (Fig. 3C),

To examine whether these gene candidates were involved in the combination treatment reduced KRAS protein expression, we used siRNA to mediate the knockdown of the gene candidates before the treatments. The upper panels in Figs. 3D to 3H showed the siRNA-mediated knockdown of the candidates (CUL7, ANAPC2, UBE2M, SYVN1, RNF123) in the CRC cells. We found that the combination treatment could significantly reduce KRAS protein level in the CUL7-, UBE2M-, SYVN1-, RNF123-knockdown cells (Fig. 3D, 3F to 3H). However, the combination treatment failed to reduce KRAS protein level in the ANAPC2-knockout cells (Fig. 3E), implying ANAPC2 was involved in the combination treatment-reduced KRAS protein expression.

We further validated the role of ANAPC2 in KRAS protein degradation. We found that ANAPC2 overexpression (Fig. 3I) significantly reduced KRAS protein levels (Fig. 3J), which was reversed in the presence of MG132 (Fig. 3J). Furthermore, we also found that the combination treatment significantly increased ANAPC2 protein expression when compared to the mono-treatments (Fig. 3K). Our data strongly suggest that the combination treatment increases ANAPC2 expression and hence increases KRAS protein degradation in the CRC cells.

The combination of WNT974 and ART induces KRAS protein degradation by increasing β-TrCP and GSK-3β expressions

We further explored whether other gene candidates would affect KRAS expressions and degradation under the combination treatment. Other studies have reported that elF5A and PEAK increased KRAS protein synthesis; NEDD4 and β-TrCP which are E3 ligase will promote KRAS degradation; SMURF2 and UBCH5 as a critical E3:E2 complex maintaining KRAS protein stability [34–36]. In our study, we found that combination treatment did not significantly affect the mRNA expressions of elF5A, NEDD4, PEAK, SMURF2, UBCH5 (Supplementary Figure S1A to S1J), but significant increased β-TrCP mRNA level (Fig. 4A and 4B) and protein level (Fig. 4C) in the CRC cells. β-TrCP is an F-box ubiquitin ligase, it has been implicated in RAS (including KRAS) ubiquitination and degradation [35, 37, 38]. β-TrCP degrades KRAS protein and in this degradation process, glycogen synthase kinase-3 beta (GSK-3β) mediates the phosphorylation of KRAS for the priming of β-TrCP to the KRAS protein for degradation [35]. Interestingly, the combination treatment not only increased β-TrCP expression but also GSK-3β expression (Fig. 4D).

We then examined whether β-TrCP would affect the KRAS protein expression in the CRC cells.

We used siRNA to mediate the knockdown of β-TrCP in the CRC cells (Fig. 4E to 4H). We found that in the β-TrCP-knockdown cells, KRAS protein expression was increased (Fig. 4I and 4J). More importantly, in these cells, in the presence of GSK-3β inhibitor, the combination treatment failed to induce KRAS ubiquitination (Fig. 4K) and reduce KRAS protein expression (Fig. 4L). Taken together, the data strongly suggest that the combination treatment increases β-TrCP and GSK-3β expressions that lead to the KRAS degradation in CRC.

The combination of WNT974 and ART exhibits a potent anti-CRC effect in vivo

Next, we examined the anti-CRC effect of the treatments with CRC-bearing xenograft mouse model. After 12-days of treatment, the combination treatment significantly reduces the tumor size (Fig. 5A and 5B) and the percentage increase in tumor size (Fig. 5C) when compared to the control and monotreatments. The combination treatment did not significantly affect the body weight of the mice (Fig. 5D), suggesting the treatments do not have apparent toxicity to the mice. In parallel with the in vitro data, the combination treatment significantly increased β-TrCP and GSK-3β expressions and reduced KRAS expression levels in the tumor tissues of these mice (Fig. 5E and 5F).

Our study shows that the combination of WNT974 and ART significantly inhibits CRC growth. Mechanistic studies suggest that the combination treatment reduces KRAS protein expressions by inducing KRAS protein degradation via the ubiquitination pathway, which may attribute to the elevated expressions of ANACP-2, beta-TrCP and GSK-3β (Fig. 6).

The development of CRC is associated with the abnormal function of cellular transmembrane signaling systems [39]. KRAS is a well-known oncogene in CRC. It is susceptible to mutations. The most common KRAS gene mutation is at glycine at G12, G13 and glutamine at Q61, while G12 has 15 different point mutations. These mutation leads to the aberrant activation of the signaling pathways including the RAF/MEK/MAPK pathway, PI3K/AKT pathway and RAGDS, RAL-RLIP pathways, leading to the malignant CRC progression and development [40, 41]. Furthermore, KRAS mutation also influences the choice of surgical techniques and is an independent predictor for positive resection margins (HR 2.44, 95%, CI 1.30–4.58, P = 0.005) [42].

Direct targeting KRAS mutant has been suggested as a therapeutic strategy. SCH-53239 was the first KRAS inhibitor that was designed to compete with GDP for the nucleotide binding site on the KRAS protein [43]. However, the efficacy was impeded by the fact that the hydrophobic pockets on the KRAS protein surface is not well-defined. Besides, the compound was toxic, and has not been approved by the Food and Drug Administration (FDA) for cancer treatment [44, 45]. Up to present, targeting KRAS for CRC treatment remains a clinical challenge [44]. Our data clearly show that the combination treatment significantly reduces CRC cell viability in eight different CRC cell lines and reduces KRAS expression and activity. These cells harbor different gene mutations including KRAS mutations in HCT116 and SW620 cells. Therefore, our data suggest that the combination treatment can be developed as KRAS-targeting therapeutics for treating CRC regardless of the mutation statuses.

Ubiquitination is an important protein posttranslational modification (PTM), it plays a crucial role in controlling protein degradation and maintaining a homeostasis [46]. Tumorigenesis involves many altered biological processes. Ubiquitination of some key signaling proteins such as RagA [47, 48], mTOR [49], PTEN [50, 51], AKT [52, 53], c-Myc [54, 55] and P53 [56, 57] have implicated in the tumor growth. Therefore, targeted ubiquitination and degradation of oncoproteins via the ubiquitin–proteasome pathway represents an alternative therapeutic strategy. By targeting the proteasome, E3 ligases, E1, E2 and deubiquitinases (DUBs), many targeted compounds have been developed to combat cancer, such as bortezomib, carfilzomib, oprozomib and ixazomib [58]. One of the most important components of the ubquitin conjugation machinery is E3 ligases that mediate protein degradation with high substrate specificity. Thus, targeting the active site of E3 enzymes or their interactions with substrates offers promising options for developing drugs with reduced side effects [59]. For example, Nedd4-1 is a general E3 ubiquitin ligase that controls the abundance of Ras. The interplay between Ras-regulated transcription of Nedd4-1 and Nedd4-1-mediated Ras degradation comprises a negative feedback regulatory loop [34]. Recently, it is found that KRAS is targeted for polyubiquitylation by E3 ligase β-TrCP and then subsequently degraded by the proteasomal degradation machinery [35, 37]. Our data show that the combination of WNT974 and ART increases β-TrCP expressions and hence KRAS protein degradation. Our study also, for the first time, revealing the regulatory role of E3 ligase ANAPC2 on KRAS protein degradation. ANAPC2 may be another therapeutic target that can increase KRAS protein degradation in CRC.

In conclusion, we report a novel discovery for the combination of WNT974 and ART in inducing KRAS protein degradation in CRC. The combination treatment significantly increases the levels of E3 ligase ANAPC2 and β-TrCP and the expression of GSK-3β, which lead to KRAS protein degradation via the ubiquitination ubiquitin–proteasome pathway. This study provides strong scientific evidence for the development of the combination of WNT974 and ART as KRAS-targeting therapeutics for CRC treatment.

CRC

colorectal cancer

ART

artesunate

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

RT-PCR

real-time polymerase chain reaction

IHC

immunohistochemistry

KRAS

kirsten rat sarcoma viral oncogene

GDP

guanosine diphosphate

GTP

Guanosine triphosphate

EGFR

epidermal growth factor receptor

PORCN

porcupine

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Fetal bovine serum

PBS

Phosphate-buffered saline

cDNA

Complementary DNA

CUL7

cullin

ANAPC2

anaphase promoting complex subunit 2

UBE2M

ubiquitin conjugating enzyme E2M

SYVN1

synoviolin 1

RNF123

RING finger protein123

elF5A

Eukaryotic translation initiation factor 5A

NEDD4

Neuronal precursor cell-expressed developmentally down-regulated 4

PEAK

pseudopodium enriched atypical kinase

SMURF2

SMAD Specific E3 Ubiquitin Protein Ligase 2

UBCH5

ubiquitin Conjugating Enzyme E2 D1

Ethical Approval

The procedures of all animal studies have granted ethics approval by the Animal Experimentation Ethics Committee of the Hong Kong Baptist University.

Consent for publication

Not applicable.

Availability of supporting data

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Competing interests

The authors declare that they have no competing interests.

Funding

This work was partially supported by Research Grant Council of HKSAR (#HKBU-22103017-ECS), Innovation & Technology Commission (#PRP/015/19FX), Natural Science Foundation of Guangdong Province (#2018A0303130122, #2021A1515010655), Health and Medical Research Fund (#08193596), HKBU FNRA-IG (#RC-FNRA-IG/20-21/SCM/01) to HYK; National Natural Science Foundation of China (#81973538)；Health and Medical Research Fund (#7182661), Key-Area Research and Development Program of Guangdong Province (#2020B1111110003) and Professor Martha Cheung Chinese Medicine Research Fund to BX.

Authors' contributions

Conceptualization: RHG, HYK, ZB. Data curation and formal analysis: RHG, MTC, CHH. Writing: RHG, HYK. Review and editing: HLXW, HYK, ZB. All authors read and approved the final manuscript.

Acknowledgements

Not applicable.

Authors` information

Affiliations

Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.

Rui-Hong Gong, Minting Chen, Chunhua Huang, Hoi Leong Xavier Wong, Hiu Yee Kwan, Zhaoxiang Bian

Corresponding authors

Correspondence to Hiu Yee Kwan or Zhaoxiang Bian.

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Table 1. PCR array genes layout
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Combination of Artesunate and WNT974 Induces KRAS Protein Degradation by Upregulating E3 Ligase ANACP2 and β-TrCP in the Ubiquitin–Proteasome Pathway

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Cell lines and culture

Western blot analysis

KRAS activity

Real time PCR analysis

siRNA transfection

CRC-bearing xenograft mouse model

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The combination of WNT974 and ART induces KRAS protein degradation in CRC cells

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