This paper presents the experimental and analytical studies to investigate the impact of concentrated laminated bamboo butt-joints on the flexural properties of vertically laminated bamboo-timber hybrid beams (VLHBs, or flitch beam). The experimental results reveal that the concentrated butt-joints significantly reduce the flexural strength of the VLHB. They also suggest that the failure mechanism of the VLHBs with or without concentrated butt-joints are completely different. In addition, laminated bamboo lumber with concentrated butt-joints was found to be unsuitable for structural applications. The analytical estimations show close agreement with the experimental results. However, due to the layout of the sample VLHB, the experimental study cannot confirm the impact of the butt-joints on the modulus of elasticity in bending.