Solid-State Optical Properties of Self-Assembling Amyloid-Like Peptides with Different Charged States at the Terminal Ends

doi:10.21203/rs.3.rs-764385/v1

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Research Article

Solid-State Optical Properties of Self-Assembling Amyloid-Like Peptides with Different Charged States at the Terminal Ends

https://doi.org/10.21203/rs.3.rs-764385/v1

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published 14 Jan, 2022

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The self-assembling of small peptides not only leads to the formation of intriguing nanoarchitectures, but also generates materials with unexpected functional properties. Oligopeptides can form amyloid-like cross-β assemblies that are able to emit intrinsic photoluminescence (PL), over the whole near-UV/visible range, whose origin is still largely debated. As proton transfer between the peptide chain termini within the assembly is one of the invoked interpretations of this phenomenon, we here evaluated the solid state PL properties of a series of self-assembled hexaphenalanine peptides characterized by a different terminal charge state. Overall, our data indicate that the charge state of these peptides has a marginal role in the PL emission as all systems exhibit very similar multicolour PL associated with a violation of the Kasha’s rule. On the other hand, charged/uncharged ends occasionally produce differences in the quantum yields. The generality of these observations has been proven by extending these analyses to the Aβ₁₆₋₂₁ peptide. Collectively, the present findings provide useful information for deciphering the code that links the spectroscopic properties of these assemblies to their structural/electronic features.

Nanoscience

Photonics/optics

Self-assembled peptide nanostructures

photoluminescence

Kasha’s rule

multicolor fluorescence emission

Peptide-based nanostructures are progressively gaining research interest as they represent innovative tools in different fields including biomedicine and biotechnology.^1–6 Depending on their primary sequence, peptides are able to self-assemble into a variety of supramolecular structures characterized by different architectures. These structures are directly affected by the secondary organization they can adopt (α-helix or β-sheet).⁷ Notably, the interest for small synthetic peptides goes well beyond their structural ability to self-assemble, as they also present intriguing functional properties including piezoelectricity,^8,9 magnetism¹⁰ and photoluminescence (PL).^11,12 This latter property consists in an unexpected optical emission exhibited by both natural and synthetic amyloid-like structures in the range 400 nm < λ_em < 560 nm upon their excitation between 380–460 nm. The first study describing this unusual phenomenon was reported in protein crystals and large polypeptides by Shukla et al.¹³ Later, very similar intrinsic blue/green photoluminescence was observed in protein fibrils or nanostructures originated by self-assembly of short peptide sequences.^14–16 Very recent studies have further expanded these observations by showing that amyloid-like peptides and proteins are able to emit fluorescence in the near infrared region upon excitation at 650–700 nm.^17,18 This strong experimental evidence is somehow in contrast with the classical frame of fluorescence properties of proteins/peptides, which states that only the three aromatic amino acids (Phe, Tyr and Trp) are able to emit fluorescence in the far UV region.¹⁹ The unusual PL properties of amyloid-like systems have been related to the β-sheet organization that characterizes these assemblies. At the state of art, several other hypotheses have been advanced to explain the origin of this PL associated with β-sheets. Nevertheless, the physicochemical basis of this phenomenon has not been identified yet, although several proposals have been reported and discussed in literature.²⁰ The most accredited hypotheses include: I) electron delocalization through intra/intermolecular hydrogen bonds;²¹ II) quantum confinement effects;²² III) charge transport through the H bond networks;^23,24 IV) carbonyl-based autofluorescence;²⁵ V) nuclear quantum effect;²⁶ VI) extensive delocalization of peptide bond electrons in the β-structure.²⁷ In 2016, Pinotsi et al. reported an interesting study in which PL emission from amyloid-like fibrils was correlated to the proton transfer between the C- and the N- termini of two adjacent strands in the β-sheet structure.^23,24 Despite an alternative explanation, involving the deformation of the peptide bond planarity in the assembly, has been successively reported,²⁷ the contribution of the proton transfer in the origin of the PL properties has not been further highlighted from the experimental point of view.²⁷ In this framework, we have recently undertaken extensive structural and spectroscopic investigations on analogous amyloidogenic systems constituted by self-assembled phenylalanine-based homopeptides, specifically in form of hexa-Phe (F6). Due to their hydrophobic nature, they were frequently conjugated to PEG (polyethylene glycol) moieties of different length to increase their solubility and to study their properties in solution (PEG8-F6, PEG12-F6, PEG18-F6 and PEG24-F6).^{21,28, 29} Moreover, by studying a series of F6 variants endowed with either free or capped N-/C- termini we have also investigated the effects that charged ends play on the aggregation mechanism and on the structural properties of the final fibers.³⁰ Since the charged state of the peptide end crucially affects the probability of proton transfer between the termini, we here perform a comparative analysis of the photoemissive properties of four peptide analogues having the same peptide sequence (F6) and different charged states of their termini (see Fig. 1). Together with the zwitterionic peptide H⁺-F6-O^-, which has charged termini, we characterized the fully capped peptide Ac-F6-Am (in which the C- and the N-termini are amidated and acetylated, respectively), and the two intermediate variants in which the termini are alternatively capped or uncapped, Ac-F6-O^- and H⁺-F6-Am. Collectively, these four variants provide all possible combinations of charged/uncharged states on the F6 peptide, thus allowing an insightful comparison of the dependence of fluorescence behaviour as function of the terminal state. Indeed, our previous structural/morphological characterization pointed out the tendency of these peptidic variants to self-assemble in β-rich nanostructures with an antiparallel orientation of the β-strands in the cross-β framework, regardless their terminal charge.³⁰ The structural resemblance of the supramolecular organization of these nanostructures indicates that potential differences in the PL properties among different peptides can only be ascribed to their different chemical properties. The solid-state spectroscopic characterization of these compounds reported in the current work unravel some interesting features such as (i) the independence of the PL emission from the charge states of the peptide termini, (ii) a multicolour PL emission, and (iii) the general violation of the Kasha’s rule.³¹ We also demonstrate that F6 films are stable against photodegradation and bleaching, an essential feature required for nanophotonic applications. The generality of the findings obtained for the F6 peptides are proven by studying the completely charged and uncharged variants of the Aβ_16-21 peptide (H⁺-Aβ_16-21-O^- and Ac-Aβ_16-21-Am), which self-assemble in amyloid-like structures analogously to F6 variants.

F6 film preparation and Scanning Electron Microscopy (SEM)

F6 peptides were synthetized by solid phase peptide synthesis using Wang or Rink amide resin, which allow to achieve carboxylated and amidated variants, respectively.³⁰ The capping of one or both the termini allows to obtain peptides with different charge states. Due to their aromatic and hydrophobic nature, all peptides are scarcely soluble in water (~0.5 mg/mL). On the contrary, they are highly soluble (up to 100 mg/mL) in HFIP (1,1,1,3,3,3-hexafluoro-2-propanol), generally used as solvent/co-solvent for highly hydrophobic Phe-containing peptides.^32,33 Only the zwitterionic form, H⁺-F6-O^-, showed a reduced solubility in HFIP (50 mg/mL). PL properties of these F6-peptides were investigated only for the samples at solid state, prepared by drop-casting peptide solutions at two different concentrations: 5 mg/mL and 100 mg/mL (50 mg/mL for H⁺-F6-O^-). Prior to PL characterization, we evaluated the morphology of the peptide nanostructures at both the concentrations by Scanning Electron Microscopy (SEM). SEM microphotos in Fig. 1 allow to observe a dependence of the morphology from the concentration. At low concentration, the four variants assemble in twisted structures with a length and a thickness between 300-1000 μm and 5-20 μm, respectively. Instead, at high concentration, SEM microphotos show a sort of film formed by very short fibrillary structures aligned along all the directions, thus suggesting a lower degree of order with respect to samples at 5 mg/mL. This decrease of order is probably due to the high volatility and rapid evaporation of the organic solvent. Although our SEM analysis are only qualitative, there seems to be a somewhat higher degree of order in the two acetylated peptides with respect to the not acetylated ones. The optoelectronic properties of the air-dried F6 samples were preliminarily studied via fluorescence microscopy. The images of fibers and films in the dark field, in the blue and green regions are reported in Fig. S1 and Fig. 2, respectively. In clear accordance with the SEM characterization, the images showed µm-long fibrillary structures and films formed by ~100 µm-wide cracked plaque structures.

Successively, the optical properties of the F6 nanostructures were extensively investigated from a quantitative point of view: from the spectral emissive viewpoint, all peptides exhibit a characteristic PL in the visible spectrum, having two main emission bands evidenced at ~400 and ~450 nm (Fig. 3a). This finding was independent of the concentration of the precipitating solution. This trace, not ascribable to the π-π stacking between the phenyl ring of Phe residues,^28,34 can rather be attributed to the presence of β sheet-rich nanostructures.^15,22

A deeper investigation of this fluorescence emission clearly indicates that all four peptides present a highly similar multicolour emission profile as evidenced by the normalized PL spectra versus the excitation wavelength in the range from 330 and 430 nm (Fig. 3b, S2). This PL behaviour was previously reported for thermally treated triphenylalanine nanodots by Rosenman et al.³² Moreover, all F6 peptides show a fluorescence emission that is almost linearly dependent from the excitation wavelength in the whole investigated range (Fig. 3c, d and Fig. S2). The variation of the emission wavelength upon increasing the excitation wavelength exhibited by these peptides represents an interesting violation of the Kasha’s rule.³¹ Indeed, a linear behaviour could be clearly evidenced for all the four F6 compounds, with estimated slopes and intercepts demonstrated as fully compatible (Table 1). In order to gain further insights into this intricate process, we measured the excitation spectra corresponding to the main PL components (λ_em = 400 nm and 450 nm). As shown in Fig. 3d, the spectra exhibit a single well-defined peak, centred at ~325 nm for all four peptides. Optical transitions at such low energy in self-assembling β-rich polypeptides/proteins have been initially correlated to a putative long-range charge delocalization along their backbone due to hydrogen bonding occurring between peptide units.¹³This hypothesis has been supported by the evidence that hydrophobic conditions (low humidity and pressure) occurring in amyloid fibrillary systems hamper both PL and charge transport.¹⁶ As a whole, the present data clearly demonstrate that this excitation-dependent emissive behaviour in the visible range as well as the low-energy excitation peak are invariant for all samples regardless their terminal charge. Therefore, in the examined systems, the photochemical explanation of this phenomenon seems not to be attributable to proton transfer or other mechanisms involving the termini of the peptide like few studies suggest.^23,24 Nevertheless, the results are coherent with very recent theoretical findings reported in the literature,²⁷ according to which the emission is promoted to the suppression of non-radiative paths due to the decreasing of the excitation energy and stabilization of n → π* transitions (i.e., electrons belonging to non-bonding pair jumping up to an antibonding π* orbital). This is shown to be the consequence of a deplanarization of amide groups, which may lead to the peculiar supramolecular organization of the examined compounds.²⁷ Moreover, other studies demonstrate that PL from H-bonds rich, non-aromatic systems arises consequently to interactions between amide groups: the abundancy of hydrogen bonding was demonstrated to bring these functionalities into close proximity.³⁵ Indeed, the results reported in the current work are in good agreement with these proposed models as they do not invoke any role for the charged ends.

The photoemissive behaviour of the F6 films is slightly modified upon increasing the peptide concentrations in the mother solution since another excitation band centred at around ~370 nm arises differently, in addition to the component at 325 nm. In this case a small but significant dependence on the terminal charge states is observed as the peak is observed only for the two peptides with the amidated C-terminal end (Fig. S3a). This experimental evidence allows to conclude that, when the peptide is prepared from highly concentrated solutions (100 mg/mL), the charge state of the terminal ends also plays a role in the fluorescence emission, attributed to Phe π-π stacking and induced by exciting the samples at 257 nm (Fig. S3b). Indeed, in these conditions, an increased emission of the peptides with the C-terminal capped ends compared to those with a charged -COO^- moiety is observed. This aggregative phenomenon arises at high concentrations of deposited material and it can still be contextualized in terms of “preferential direction” in the self-assembling. It is reasonable to conclude that the main contribution to the optical response of the F6 films, in the higher concentration regime, comes from the stacking of the huge number of aromatic residues. This analysis is also supported by the SEM images above discussed for the samples at high concentrations. Finally, measurements of fluorescence quantum yield (PLQY) at the two excitation wavelengths of 325 and 370 nm (Table 2) provide further insights into the PL emission of these systems. At 325 nm an increase of the average PLQY from ~5(1)% to ~10(2)% is evidenced when going from 5 to 100 mg/mL. No specific effect of the charged/uncharged ends is observed for this PL increase. Although it is important to point out that PLQY only provides information about the integrated photoemissive behaviour of the samples and not about the exact origin and attribution of the spectral features of the samples. This finding points out that the concentration of the peptide in the stock solution has an impact on the resulting structure of the solid and on the related PL properties. The inspection of the PLQY obtained upon excitation at 370 nm, which has only been evaluated in the samples at higher concentrations, indicates some role of the charge state of the terminal as C-terminal capped variants present quantum yields higher than those exhibited by peptide containing –COO^- groups.

Photobleaching study

Having shed light upon the intriguing spectroscopic properties of the nanostructures formed by F6 peptides, we evaluated their robustness against photodegradation and bleaching, which represent essential features to pursue any nanophotonic application. For this experiment, fluorescence spectra were collected and monitored after continuous exposure to the 330 nm excitation line, keeping the sample mounted in the integrating sphere and the excitation shutter open, for up to 180 mins at an incident power of ~820 μW. Assuming the interchangeability of the four variants in view of the previous results, the measurements were solely carried out on the Ac-F6-Am peptide film at 100 mg/mL concentration. After 3 h continuous exposure to UV light, a 64% retaining of the integrated PL intensity of the sample could be evidenced, revealing a remarkable bleaching resistance (Figure 4a). A rough estimation of the bleach rate, performing a single-exponential decay fit, led to a value of 7.2 (1.1) h, which is much higher than that of any conventional protein dye (whose characteristic range is ~10¹-10² s).³⁶ Furthermore, the two deconvolved components of the PL spectrum (peaked at ~400 and ~450 nm) interestingly exhibit a different bleaching kinetics, highlighting the different origin of their photoemissive mechanisms (Fig. 4b). Moreover, no recovery was evidenced after 40 mins in dark (data not shown).

Photoemissive properties of Aβ_16-21peptides

In order to demonstrate that the PL results collected for F6 nanostructures can be generalized to other classes of β-sheet-assembling peptides, we investigated another amyloidogenic system. Specifically, for this study we choose the sequence 16-21 “extracted” from the Aβ_1-42peptide (Aβ_16-21), in its fully neutral (Ac-Aβ_16-21-Am) and zwitterionic state (H⁺-Aβ_16-21-O^-)(see Figure S4a). Analogously to their parental peptide Ac-Aβ_16-20-Am,³⁷ the structural characterization on Aβ_16-21 variants (SEM and ThT assay) confirmed their capability to self-organize in β-sheet-rich nanostructures (see Figure S4b and S4c). The PL characterization of Aβ-peptide films at 5 and 100 mg/mL was carried out using the same setting chosen for the F6 samples. Due to the increased solubility in water of Aβ variants respect to F6 ones, samples at 5 mg/mL were prepared from their aqueous solutions. Analogously to the F6-peptides, PL microscopy images of Aβ (drop-casted from a solution at 5 mg/mL in H₂O) show emissive fibrils in the blue spectral region (Fig. 5a). From the spectral viewpoint, both the Aβ variants show an emission peak around 400 nm (Fig. 5b), a multicolour PL emission profile in the range 330-400 nm (Fig. 5c) and a non-trivial dependence of the emission maximum on the excitation wavelength (Fig. 5d). Moreover, both the samples at 5 and at 100 mg/mL show a low-energy excitation peak at 325 nm (λ_em = 400 nm), which is compatible with what observed in F6 samples at the same concentration (Fig. 5e). The measured PLQYs at 100 mg/mL were (4.5 ± 0.2)% and (3.00 ± 0.15)% for H⁺-Aβ_16-21-O^- and Ac-Aβ_16-21-Am, respectively. Such values are comparable to those measured for the F6 variants in the same experimental conditions, as this peculiar emission turned out to exhibit a quite high efficiency. Altogether, such analyses support and are coherent with the assumption made for the F6, i.e., that the excitation-dependent PL from an amyloidogenic system may be a direct consequence of a peculiar tridimensional-spatial disposition of the amide groups consequent to the β-sheet arrangement.

The PL results collected on two amyloidogenic systems, F6 and Aβ_16-21, allow to conclude that all the generated peptide films are able to emit multicolor PL profiles regardless their terminal charge state. The observed optical properties provide further support to the notion that proton transfer between the C- and the N-terminal of two adjacent strands in a β-sheet structure is not essential for generation of the intrinsic PL exhibited by amyloid-like systems. On the other hand, these results could also experimentally support the hypothesis that the emission is a consequence of the decreasing of the excitation energy and stabilization of the peptide bond, due to a deplanarization of amide groups.²⁷ The efficient excitation-dependent PL of the examined compounds was investigated and demonstrated to be retained even at higher concentrations where other aggregation-induced emissive phenomena occur, reaching PLQY values of the order of ~ 10%. The origin of this visible PL trace seems to be a direct consequence of their peculiar 3D-spatial disposition at the very first-stage supramolecular level.

Protected N^α-Fmoc-Phe-OH, preloaded Fmoc-Phe-Wang resin, Rink amide MBHA (4-methylbenzhydrylamine) and all reagents for coupling reactions, commercially available from Calbiochem-Novabiochem (Laufelfingen, Switzerland), were used. All other chemicals materials, commercially available by Sigma-Aldrich (Milan, Italy), Fluka (Bucks, Switzerland) or LabScan (Stillorgan, Dublin, Ireland) were used as received unless otherwise stated.

Peptide synthesis

F6-homosequences were synthesized according to the method previously described.³⁰ Ac-Aβ_16-21-Am and H⁺-Aβ_16-21-O^- were synthesized using the standard solid-phase 9-fluorenylmethoxycarbonyl (Fmoc) procedures. The Rink amide MBHA resin (substitution 0.65 mmol/g, 010 mmol) and the Wang resin preloaded with Ala (substitution 0.60 mmol/g, 010 mmol) were used as solid phase support. Wang resin provides C-terminus as carboxylic acid, meanwhile Rink amide resin releases peptide as amide at the C-terminus. The synthesis was carried out using a mixture of N,N-dimethylformamide/N-methyl-2-pyrrolidone (DMF/NMP, 1:1, v/v) as solvent phase. Before starting the peptide elongation, resins were swelled for 30 min in solvent medium. Fmoc deprotection was performed twice (each treatment for 10 min) using 30% (v/v) piperidine in DMF/NMP. The Fmoc-amino acid couplings were achieved by adding 2-fold molar excess of Fmoc-aa-OH, mixed with equimolar amounts of 1-hydroxybenzotriazole (HOBt), benzotriazol-1-yl-oxy-tris-pyrrolidino-phosphonium (PyBop) and 4-fold molar excess of diisopropylethylamine (DIPEA). All couplings were performed twice for 40 minutes. N-terminus acetylation was performed twice (each treatment for 10 minutes) using a solution of pyridine/acetic anhydride (4/4.7 v/v) in DMF. Crude peptides were fully cleaved in acidic condition by TFA (trifluoroacetic acid)/H₂O/ TIS (triisopropylsilane) (90/5/5 v/v/v) mixture at room temperature for 2 hours. Then, peptides were precipitated with ice-cold ether and lyophilized. Purity and chemical identity of the synthetic products were assessed by analytical LC-MS analyses by using Finnigan Surveyor MSQ single quadrupole electrospray ionization (Finnigan/Thermo Electron Corporation San Jose, CA) using a column C18-Phenomenex eluted with an H₂O/0.1% TFA (A) and CH₃CN/0.1% TFA (B) from 5% to 70% over 20 minutes at 200 μL·min^-1 flow rate.

H⁺-Aβ_16-21-O^- characterization: t_R = 15.00 min, MS (ESI+): m/z 723.90 calcd. for C₃₈H₅₇N₇O₇: [M+H⁺] = 724.90

Ac-Aβ_16-21-Am characterization: t_R = 15.46 min, MS (ESI+): m/z 764.95 calcd. for C₄₀H₆₀N₈O₇: [M+H⁺] = 764.46

Sample preparation

Ac-F6-O^-, H⁺-F6-Am and Ac-F6-Am solutions were prepared dissolving peptide powders directly in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) at 100 mg·mL^-1. For H⁺-F6-O^-, solution concentration was 50 mg·mL^-1. Subsequently, these solutions were properly diluted with HFIP at the final required concentration. Ac-Aβ_16-21-Am peptide solution was prepared directly dissolving lyophilized powder in double distilled water. The experimental concentration was assessed by UV-Vis spectroscopy carried out on UV-Vis Thermo Fisher Scientific Inc (Wilmington, Delaware USA) Nanodrop 2000c spectrophotometer using a 1.0 cm quartz cuvette (Hellma) and a molar absorptivity (ε257) of 390 M^-1·cm^-1. Then, these peptide solutions were drop-casted onto flat microscope glass slides and allowed to air dry at room temperature.

Thioflavin T (ThT) assay

Aggregate formation of H⁺-Aβ_16-21-O^- and Ac-Aβ_16-21-Am was assessed by fluorescence spectroscopy using ThT. This dye associates rapidly with β-rich aggregates giving rise to an enhanced fluorescence emission at 482 nm.³⁸ Spectra of an aqueous solution of 25 µM ThT, before and after the addition of Ac-Aβ_16-21-Am derivative (3.0 mg/mL), were recorded at room temperature. The spectrum of the peptide solution alone was also acquired as reference. Fluorescence measurements were recorded between 460 and 650 nm exciting the samples at 450 nm.

Scanning Electron Microscopy (SEM)

Morphological analysis of the nanostructures was carried out using field emission scanning electron microscope (Nova NanoSem 450-FEI). Briefly, 10μL of sample solutions at 100.0 (50 mg/mL for H⁺-F6-O^-) and 5.0 mg/mL were dropped off on aluminum stub using a graphite adhesive tape. A thin coat of Au and Pd was sputtered at a current of 20 mA for 120 s. The sputter coated samples were then introduced into the specimen chamber and the images were acquired at an accelerating voltage of 2-5 kV, spot 3, through the Everhart Thornley Detector (ETD) and the Through the Lens Detector (TLD).

Fluorescence microscopy

10 μL of F6 and of Aβ_16-21peptide solutions were drop-casted on a clean coverslip glass, dried and imaged with fluorescence microscopy. Immunofluorescence images were taken with a Leica DM6 M fluorescence microscope equipped with DFC 7000 T camera, at excitation wavelengths 365 nm and 470 nm.

Fluorescence measurements

The as-prepared samples were spectrally characterized using a Jasco FP-8300 spectrofluorometer equipped with an ILF-835 integrating sphere, which allowed to evaluate the photoluminescence quantum yield (PLQY) with the absolute method. Fluorescence spectra of the four F6 films were acquired in a window up to 650 nm, probing the 330 nm – 430 nm excitation range at 10 nm steps. All acquisitions were averaged over five measurements after spectral correction.

Photodegradation of peptide films

PL spectra were collected after continuous exposure to the 330 nm excitation line of the instrument, keeping the sample mounted in the integrating sphere and the excitation shutter open, for up to 180 mins (at an incident power of ~820 μW).

AUTHOR INFORMATION

*Corresponding Author: Antonella Accardo

E-mail: [email protected]. Tel: +39-081-2532045

Present Address: Department of Pharmacy, University of Naples “Federico II”, Via Mezzocannone, 16 – I-80134 Napoli, Italy.

*Corresponding Author: Raffaele Velotta

Email: [email protected]. Tel: +39-081- 676148

Present Address: Department of Physics “Ettore Pancini”, University of Naples “Federico II”, Via Cintia 26, Naples, 80125, Italy.

Notes

The authors declare no competing financial interest.

Author contributions A.A., L.V. and R.V. coinceived the study. E.G. synthesized peptide conjugates, C.D. prepared peptide solutions, performed structural characterization experiments and discussed data. AA and G.M. analyzed, results. C.S. and B.D.V. performed fluorescence and photobleanching experiments. C.S. acquired immunofluorescence images and analyzed the fluorescence data. AA, R.V. and L.V. discussed the results and wrote the paper in close collaboration with all the authors.

Conflicts of interest

There are no conflicts to declare.

Additional Information

Supplementary information accompanies this paper at http://www.nature.com/srep

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Table 1: Summary of the estimated quantities of the linear fits reported in Figure 3c together with their relevant statistic parameters.

Sample	Slope	Intercept	Reduced χ²	Adj. R²
Ac-F6-Am	0.97 ± 0.06	80 ± 23	1.08	0.962
H⁺-F6-Am	0.98 ± 0.06	74 ± 23	1.14	0.962
Ac-F6-O^-	1.00 ± 0.05	69 ± 18	1.125	0.976
H⁺-F6-O^-	0.97 ± 0.06	80 ± 23	1.135	0.962

Table 2: Summary of the measured values of PLQY of all F6 samples at the different concentrations.

Sample	F6 concentration (mg/mL)	QY at 325 nm (%)	QY at 370 nm (%)
Ac-F6-Am	5	5.8 ± 0.3	--
Ac-F6-Am	100	12.1 ± 0.6	6.3 ± 0.3
H⁺-F6-Am	5	3.6 ± 0.2	--
H⁺-F6-Am	100	11.3 ± 0.6	9.1 ± 0.5
Ac-F6-O^-	5	5.3 ± 0.3	--
Ac-F6-O^-	100	10.0 ± 0.5	4.4 ± 0.2
H⁺-F6-O^-	5	4.4 ± 0.2	--
H⁺-F6-O^-	50	12.7 ± 0.6	2.5 ± 0.1

No competing interests reported.

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Solid-State Optical Properties of Self-Assembling Amyloid-Like Peptides with Different Charged States at the Terminal Ends

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