Tissue-wide coordination of polarized cytoskeletal organization and cell behaviour, critical for organ morphogenesis and tumour progression, is controlled by asymmetric membrane localization of non-canonical Wnt/planar cell polarity (PCP) signalling components. Understanding the dynamic regulation of PCP thus requires visualization of these core proteins. In vertebrates, immunohistochemical studies have provided snapshots of PCP within tissues while exogenous, fluorescently-tagged reporters have been introduced for live imaging of cell polarity. However, the functionality and spatiotemporal relevance of static and exogenous PCP readouts remain uncertain. Here we fluorescently tag an endogenous core PCP component, Vangl2, in zebrafish. We report on the authentic regulation of vertebrate PCP, through live imaging of this native sfGFP-Vangl2 protein during embryogenesis. We couple sfGFP-Vangl2 with conditional zGrad GFP-nanobody degradation methodologies for tissue-specific interrogation of PCP function. Together, our studies provide crucial insights into the establishment and maintenance of vertebrate PCP and create a powerful experimental paradigm for future investigations.