Lentinan Administration Alleviates Diarrhea of Rotavirus-Infected Weaned Pigs via Regulating Intestinal Immunity

doi:10.21203/rs.3.rs-76696/v1

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Lentinan Administration Alleviates Diarrhea of Rotavirus-Infected Weaned Pigs via Regulating Intestinal Immunity

https://doi.org/10.21203/rs.3.rs-76696/v1

This work is licensed under a CC BY 4.0 License

Journal Publication

published 09 Mar, 2021

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Background

This study aimed to verify whether lentinan (LNT) administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs.

Methods

Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15.

Results

Dietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P < 0.05). The polysaccharide was isolated from LNT product, whose molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infected IPEC-J2 cells, this polysaccharide significantly increased cell viability (P < 0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P < 0.05).

Conclusion

Those results suggest that LNT administration increases the piglets’ resistance to RV-induced stress, likely by supporting intestinal immunity.

Animal Science

lentinan

weaned pigs

IPEC-J2 cells

rotavirus

gut immunity

Shiitake mushroom can improve the immunologic function, and has been used to prevent and treat various diseases of humans and animals for thousands of years in China and Japan [1]. Lentinan (LNT) is one of the major compounds purified from this mushroom [2]. Many researchers have reported that lentinan has antitumor, anti-inflammatory, antioxidant and antiviral functions via improving immunity [3–6].

Rotavirus (RV) is a major pathogen that induces severe diarrhea in infants and young animals. After ingestion, RV particles invade the non-dividing mature enterocytes of small intestine, induce the inflammatory response, and even cause dysfunction of immune system in the children and weaned pigs [7–9]. Recently, RV vaccine has also been studied by many researchers, but, so far, there is no effective vaccine because of the complexity of the species [10, 11]. Our previous study has shown that LNT administration can reduce RV-infected diarrhea, and improve intestinal barrier function [12]. Then, based on the capacity of enhancing immunity, LNT may be considered as an interesting food and feed ingredient that could be of interest to support human and animal performance in RV-induced stress conditions. However, there are no relative studies.

Therefore, via the in vivo and in vitro experiments, this study was conducted to test the hypothesis that LNT administration might improve growth performance and resilience in RV-infected piglets. And, in this process, the effect of intestinal immune function will also be analyzed.

Animal trial

Animals and diets

The experimental protocol was approved by Animal Care Advisory Committee of Sichuan Agricultural University. Twenty-eight barrows (Duroc × Landrace × Yorkshire) with an initial average BW of 7.51 ± 1.37 kg were individually housed in metabolic cages (1.5 m × 0.7 m × 1.0 m). The pigs were fed with the experimental diets 4 times daily at 08:00, 12:00, 16:00 and 20:00, and could have access to water ad libitum.

The basal diet was formulated to meet NRC (2012) nutrient recommendations for pigs (7–11 kg). Its composition and nutrient levels were shown in Supplementary Table 1. The LNT diet was formed by supplementing 84 mg/kg LNT product in the basal diet. This LNT product was purchased from Sichuan Hengruitongda Biotechnology Co. LTD. The active components (lentinan) accounted for 30% of this LNT product.

Experimental design and sample collection

Following 3d of acclimatization, all pigs were divided randomly into two groups on basis of initial body weight and litter origin (n = 14), and were fed with the basal and LNT-supplemented diets, respectively. The experimental duration was 19 d. From the 12th to 14th day, nutrient digestion was estimated as described previously [13].

RV preparation and virus titre determination (tissue culture infective dose 50 (TCID₅₀) value) were executed as described previously [14].

On the 15th day, all pigs were infused 5 mL of sterile 100 mmol/L NaHCO₃ solution. Then, half of pigs in two groups were gavaged orally with 4 mL (10⁶ TCID₅₀/mL) of RV, whereas the other pigs received 4 mL of the sterile essential medium by oral gavage. Following RV infusion, the diarrhea symptom of all pigs was observed and recorded in each day. Fecal consistency was scored: 0, normal; 1, pasty; 2, semiliquid; 3, liquid. Diarrhea happened when fecal consistency score of pigs is ≥ 2 [15]. Average daily weight gain (ADG), average daily feed intake (ADFI) and feed conversion of all pigs were calculated via measuring feed intake in each day and body weight on d 1, 15 and 20.

On the 20th day, after 12 h starvation, all pigs were weighted. Blood samples of all pigs were collected from jugular vein, centrifuged at 3500 g for 10 min, and serum was gathered. Then, the pigs were refed for 1.5 h. Following refeeding, all pigs were euthanized by intracardially injecting Na pentobarbital (50 mg/kg of body weight) and exsanguinated. The jejunum was immediately separated, and cleaned with ice-cold saline. Jejunal mucosa were gathered by scraping the gut wall with a glass microscope slide, frozen in liquid nitrogen, and stored at -80 °C until analysis.

Analysis of nutrient digestibility

Ash insoluble in hydrochloric acid was used as non-absorbable digestion marker in the digestion trial. Dry matter (DM), crude protein (CP), ether extract (EE), calcium (Ca), phosphorus (P), gross energy (GE) and ash insoluble in hydrochloric acid in feces and feeds were analyzed on the basis of the method of Diao et al. (2016) [13]. Nutrients digestibility was calculated as [100–100 × (A × D) / (B × C)]; A and B are the content of ash insoluble in hydrochloric acid in feeds and feces, respectively; C and D are the content of nutrients in feeds and feces, respectively.

Analysis of urea nitrogen (SUN), rotavirus antibody (RV-Ab), immunoglobulin (Ig) and/or interferon-beta (IFN-β) levels in serum and jejunal mucosa

Jejunal mucosa (about 100 mg) were added to ice-cold PBS, shattered at 4 °C, and then centrifuged at 5000 g for 15 min at 4 °C. The supernatant was used to measure the levels of RV-Ab, secretory immunoglobulin A (sIgA) and IFN-β.

The SUN level was measured with the kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instruction. The RV-Ab concentrations in serum and jejunal mucosa were detected by using ELISA kit (TSZ ELISA, Framingham, MA). Serum IgA, IgG and IgM concentrations, and the concentrations of sIgA and IFN-β in the jejunal mucosa were detected with ELISA kits from Nuoyuan Co. Ltd. (Shanghai, China).

Analysis of expressions of host defense peptides in the jejunal mucosa

Total RNA in the jejunal and ileal mucosa was isolated with TRIZOL reagent (TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China) according to the manufacturer’s instructions. By using DU 640 UV spectrophotometer detection (Beckman Coulter Inc., Fullerton, CA), the OD₂₆₀:OD₂₈₀ ratio of all samples ranged from 1.8 and 2.0, which indicated good quality RNA. The integrity of RNA was further analyzed by 1% agarose gel electrophoresis. Using RT Reagents (TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China), RNA of all samples was reversely transcribed into complementary DNA according to the manufacturer’s instructions. The expression levels of porcine beta-defensin 1 (pBD1), pBD2, pBD3 and β-actin in the jejunal mucosa were analyzed by real-time quantitative PCR using SYBR Premix Ex Taq reagents (TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China) and CFX-96 Real-Time PCR Detection System (Bio-Rad Laboratories, Richmond, CA) as described previously [16]. The primers of all genes, listed in Supplementary Table 2, were purchased by TaKaRa Biotechnology (Dalian) Co., Ltd. (Dalian, China). Relative gene expression to reference gene (β-actin) was determined in order to rectify the variance in amounts of RNA input in the reaction. Then, the relative gene expressions were calculated with the previous method [17].

Extraction and analysis of LNT polysaccharides

Preparation of water-soluble polysaccharides

The water-soluble polysaccharides were prepared as previously described with some modifications [18]. Briefly, the LNT product (20 g) provided by Sichuan Hengruitongda Biotechnology Co. LTD. was dissolved in 200 mL distilled water at 85 °C for 3 h. The supernatants were concentrated to 1/5 of the original volume in a rotary vacuum evaporator at 50 °C under reduced pressure. The proteins in supernatants were removed by adding the Sevag reagent (chloroform : n-butanol, 4 : 1 (v/v)), and the liquid was dialyzed (3000 Da) with ultra-pure water for 48 h. Then, the solution was precipitated with 4 volume of 95% ethanol (v/v) at 4 °C for 24 h, and the precipitates were collected by centrifugation (2655 × g, 10 min). Following lyophilization, the polysaccharides were produced. The purity of polysaccharides was measured according to previously published-methods [19].

Molecular weight analysis

The molecular weight (Mw) distributions were measured using high performance gel permeation chromatography (HPGPC) with an Agilent 1100 HPLC system equipped with a Waters 2410 refractive index detector and a TSK-GEL G5000 PWxL column (7.8 mm × 300 mm, TOSOH Co., Japan) as previously described [20]. The mobile phase consisted of ultrapure water which flowed at a rate of 0.8 mL/min and a temperature of 30 °C. The polysaccharide solution sample (20 µL, 2.0 mg/mL) was added in each run. The standard curve was created using Dextran standards (3.0 to 670 kDa, Sigma-Aldrich Co. LLC., USA).

Monosaccharide composition

The monosaccharide composition in polysaccharide samples was determined by using gas chromatography according to a previous method [20]. Briefly, the dried samples (10 mg) were hydrolyzed with 2 mL of trifluoroacetic acid (2 mol/L) at 110 °C for 2 h, and the solution was lyophilized after removing the excess acid. Then, hydroxylamine hydrochloride (10 mg) and pyridine (0.5 mL) were added to the hydrolysate, and this solution was incubated for 30 min at 90 °C. Following cooling to room temperature, acetic anhydride (0.5 mL) was added and reacted for 30 min in a water bath (90 °C). Standard monosaccharide samples (D-glucose, D-galactose, L-arabinose, D-mannose, L-rhamnose, and D-xylose (Sigma-Aldrich Co. LLC., USA)) were derivatized under the same conditions. The monosaccharide composition was identified by comparison with the retention times of monosaccharide standards.

Cell cultural trial

Cell culture

The IPEC-J2 cell line (porcine intestinal epithelial cells) was kindly supplied by Dr. Guolong Zhang at Oklahoma State University (Stillwater, OK, USA), and was cultured as described previously [15]. Briefly, IPEC-J2 cells were cultured with DMEM/F12 medium (Gibco Laboratories Life Technologies Inc., Grand Island, NY, USA) with 10% fetal bovine serum (Hyclone Laboratories Inc., Logan, UT, USA), 1% antibiotics (Hyclone Laboratories Inc., Logan, UT, USA) and 0.2% insulin-transferrin-selenium (Lonza, Walkersville, MD, USA) at 37 °C in 5% CO₂.

Cell viability assay

The viability of IPEC-J2 cells was measured with the Cell Counting Kit-8 (CCK8; Beyotime, Jiangmen, China) according to the manufacturer’s instructions. In brief, IPEC-J2 cells were seeded in 96-well plates at 1.25 × 10⁴ cells/well. After 20 h, the varying concentrations (0.0, 1.5, 3.0, 6.0, 12.0 and 24.0 mg/L) of LNT polysaccharide were added to the cells (n = 9). At 6, 12 and 24 h following addition of LNT, CCK8 solution was added and incubated for 2 h. Cell viability was analyzed with a BioTek Synergy HT microplate reader (BioTek Instruments, Winooski, VT, USA) at an absorbance of 450 nm. This will get the suitable treating-dose and -time of LNT polysaccharide in following cell trials.

Cell experiments

The viability of IPEC-J2 cells that were supplemented with LNT polysaccharide and RV was also analyzed by CCK8. Briefly, IPEC-J2 cells were seeded in 96-well plates at 1.25 × 10⁴ cells/well. After 20 h, 0 or suitable dose of LNT polysaccharide were added to the cells for suitable exposure-time (n = 24). The media were removed, and cells were washed three times with PBS and incubated with media or RV (multiplicity of infection (MOI) = 3) for 1 h (n = 12). Following inoculum removal and washing twice with PBS, IPEC-J2 cells were supplemented with 0 or suitable dose LNT polysaccharide for 4 d. On d 1, 2, 3 and 4 d, CCK8 solution was added and incubated for 2 h. Cell viability was analyzed with a BioTek Synergy HT microplate reader (BioTek Instruments, Winooski, VT, USA) at an absorbance of 450 nm.

IPEC-J2 cells were seeded in 6-well plates at 2.5 × 10⁵ cells/well. Following 20 h, cells were exposed to the suitable dose and time of LNT polysaccharide at 37 °C in 5% CO₂. Then, the media were removed, and cells were washed three times with PBS and incubated with RV (MOI = 3) for 1 h. After inoculum removal and washing twice with PBS, IPEC-J2 cells were supplemented with the suitable dose LNT polysaccharide for a further 3 d. All cells and supernatants were collected.

Analysis of rotavirus non-structural protein 4 (NSP4) in cell supernatants

The concentration of NSP4 in cell supernatants was detected with a commercially available porcine ELISA kit (TSZ, Framingham, MA, USA) according to the manufacturer’s instructions.

Analysis of gene expressions in IPEC-J2 cells

RNA was isolated from cells by TRIZOL reagent (TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian, China) according to the manufacturer’s instructions. The methods of RNA concentration and integrity analysis, complementary DNA synthesis, real-time quantitative PCR and relative gene expression calculation were consistent with those in the animal experiment. The primers of all genes, listed in Supplementary Table 2, were purchased by TaKaRa Biotechnology (Dalian) Co., Ltd. (Dalian, China).

Statistical analysis

All experimental data were processed with Microsoft Excel 2013, analyzed by using SAS (version 8.1; SAS Institute, Gary, NC), and indicated as means with their standard errors (SE). (1) Animal trial. The data of growth performance before RV challenge and nutrient digestibility in weaned pigs were analyzed with the unpaired t test. And except these and diarrhea rate, the other data were analyzed as a 2 × 2 factorial with the general linear model procedures of the Statistical Analysis Package. The model factors contained the effects of LNT (basal or LNT-supplemented diets), RV challenge (infusing essential medium or rotavirus), and their interaction. (2) Cell-culture trial. The cell viability was analyzed with the unpaired t test. The other data were analyzed using one-way ANOVA, followed by Duncan’s Multiple Range test. The P value less than 0.05 was deemed statistical significance while the P value less than 0.10 was deemed statistical trends.

Growth performance, SUN, and nutrient digestibility of weaned pigs

During the first 2 weeks, supplementing LNT in the diet reduced feed conversion of weaned pigs (P < 0.05, Table 1). RV challenge increased feed conversion and SUN (P < 0.05), and tended to reduce ADG (P = 0.08) in weaned pigs (Table 1). After RV infusion, LNT administration elevated ADG, and decreased feed conversion and SUN in weaned pigs (P < 0.05, Table 1). Moreover, in the RV-infected pigs, dietary LNT supplementation enhanced ADG, and decreased feed conversion and SUN (P < 0.05, Table 1).

Table 1

The effect of dietary LNT supplementation and/or RV challenge on growth performance and serum urea nitrogen level in weaned pigs
	- RV		+ RV		P value
	CON	LNT	CON	LNT	RV	LNT	LNT × RV
1–14 d
ADFI (g)	364.96 ± 29.35	387.99 ± 26.00				0.56
ADG (g)	237.80 ± 20.06	281.25 ± 17.53				0.11
Feed conversion	1.55 ± 0.04^a	1.38 ± 0.04^b				< 0.05
15–19 d
ADFI (g)	450.11 ± 45.71	466.63 ± 20.89	425.50 ± 42.22	498.55 ± 21.31	0.60	0.13	0.26
ADG (g)	304.29 ± 23.92^b	381.25 ± 25.16^a	239.58 ± 28.40^c	354.58 ± 18.88^b	0.08	< 0.05	0.45
Feed conversion	1.48 ± 0.11^b	1.24 ± 0.07^c	1.82 ± 0.10^a	1.50 ± 0.05^b	< 0.05	< 0.05	0.67
SUN (mmol/L)	6.09 ± 0.05^c	5.65 ± 0.07^d	7.35 ± 0.18^a	6.51 ± 0.09^b	< 0.05	< 0.05	0.36
- RV, infusing the essential medium; + RV, infusing the porcine rotavirus; CON, basal diet; LNT, LNT-supplemented diet; ADFI, average daily feed intake; ADG, average daily gain; F/G, feed/gain ratio; SUN, serum urea nitrogen.
^{a, b, c} Mean values within a row with unlike superscript letters were significantly different (P < 0.05).

Before RV challenge, dietary LNT supplementation increased the digestibility of DM, CP, EE, Ca, P and energy in weaned pigs (P < 0.05, Fig. 1).

The diarrhea rate and RV-Ab levels of serum and jejunal mucosa in weaned pigs

Infusing the essential medium did not induced diarrhea, but oral RV gavage led to diarrhea in weaned pigs (Fig. 2). RV-Ab levels of serum and jejunal mucosa in weaned pigs were increased by RV challenge (P < 0.05, Table 2). Additionally, in the weaned pigs infused with RV, LNT administration decreased the diarrhea rate, and shortened the diarrhea duration (Fig. 2), but further enhanced serum RV-Ab level (P < 0.05, Table 2).

Table 2

The effect of dietary LNT supplementation and/or RV challenge on the immunoglobulin, rotavirus-antibody and/or interferon-β concentrations in serum and jejunal mucosa of weaned pigs
	- RV		+ RV		P value
	CON	LNT	CON	LNT	RV	LNT	LNT × RV
Serum
IgM (mg/mL)	0.17 ± 0.02^c	0.24 ± 0.01^b	0.26 ± 0.01^b	0.35 ± 0.03^a	< 0.05	< 0.05	0.77
IgG (mg/mL)	1.26 ± 0.04^b	1.49 ± 0.10^a	1.59 ± 0.06^a	1.64 ± 0.08^a	0.07	< 0.05	0.25
IgA (µg/mL)	68.58 ± 2.50^c	73.23 ± 1.54^b	76.25 ± 1.21^ab	81.24 ± 1.21^a	< 0.05	< 0.05	0.92
RV-Ab (ng/L)	17.23 ± 2.07^c	18.29 ± 0.99^c	22.14 ± 1.07^b	26.79 ± 1.15^a	< 0.05	< 0.05	< 0.05
Jejunal mucosa
sIgA (µg/mg protein)	19.25 ± 1.23^b	25.17 ± 1.52^a	15.23 ± 0.82^c	19.72 ± 0.92^b	< 0.05	< 0.05	0.58
RV-Ab (ng/mg protein)	7.71 ± 0.57^b	8.79 ± 0.72^ab	8.82 ± 1.04^ab	9.80 ± 0.55^a	< 0.05	< 0.05	0.86
IFN-β (pg/mg protein)	225.31 ± 26.48^c	353.65 ± 18.58^b	401.87 ± 25.57^b	508.88 ± 16.95^a	< 0.05	< 0.05	0.64
- RV, infusing the essential medium; + RV, infusing the porcine rotavirus; CON, basal diet; LNT, LNT-supplemented diet; IgA, immunoglobulin A; IgG, immunoglobulin G; IgM, immunoglobulin M; RV-Ab, rotavirus-antibody; sIgA, secretory immunoglobulin A; IFN-β, interferon-β.
^{a, b, c} Mean values within a row with unlike superscript letters were significantly different (P < 0.05).

Serum immunoglobulin concentrations, and sIgA and IFN-β levels and host defense peptide expressions of jejunal mucosa in weaned pigs

Oral RV gavage increased serum IgM (P < 0.05), IgG (P = 0.07) and IgA (P < 0.05) levels, enhanced IFN-β level of jejunal mucosa (P < 0.05), reduced sIgA level of jejunal mucosa (P < 0.05), and inhibited pBD1, pBD2 and pBD3 mRNA expressions of jejunal mucosa (P < 0.05) in weaned pigs (Tables 2 and 3). Dietary LNT supplementation enhanced serum IgM, IgG and IgA levels (P < 0.05), increased sIgA and IFN-β concentrations of jejunal mucosa (P < 0.05), and stimulated pBD1, pBD2 and pBD3 mRNA expressions of jejunal mucosa (P < 0.05) in weaned pigs (Tables 2 and 3). Additionally, in the RV-challenged pigs, LNT administration further increased serum IgM concentration and IFN-β level of jejunal mucosa, and alleviated the effect of RV challenge on sIgA level, and pBD1, pBD2 and pBD3 mRNA expressions of the jejunal mucosa (P < 0.05, Tables 2 and 3).

Table 3

The effect of dietary LNT supplementation and/or RV challenge on mRNA expressions of host defense peptides in the jejunal mucosa of weaned pigs
	- RV		+ RV		P value
	CON	LNT	CON	LNT	RV	LNT	LNT × RV
pBD1	1.00 ± 0.09^b	1.22 ± 0.03^a	0.45 ± 0.04^d	0.79 ± 0.05^c	< 0.05	< 0.05	0.30
pBD2	1.00 ± 0.06^a	1.13 ± 0.07^a	0.51 ± 0.05^c	0.79 ± 0.03^b	< 0.05	< 0.05	0.20
pBD3	1.00 ± 0.09^b	1.27 ± 0.06^a	0.58 ± 0.02^c	0.94 ± 0.05^b	< 0.05	< 0.05	0.52
- RV, infusing the essential medium; + RV, infusing the porcine rotavirus; CON, basal diet; LNT, LNT-supplemented diet; pBD1, porcine beta-defensin 1; pBD2, porcine beta-defensin 2; pBD3, porcine beta-defensin 3.
^{a, b, c, d} Mean values within a row with unlike superscript letters were significantly different (P < 0.05).

Primary characteristics of LNT polysaccharides

Following purification, the purity of polysaccharide from LNT product is 95%. Via analysis, in the HPGPC chromatogram of LNT polysaccharide, there was only one peak, and the Mw was 5303 Da, which indicated that the LNT polysaccharide was possibly pure.

The monosaccharide composition supplies important information for the polysaccharide structure [21], and was also analyzed. Three major monosaccharides including glucose, arabinose and galactose were identified in the LNT polysaccharide while rhamnose, xylose and mannose were also detected. The contents of glucose, arabinose, galactose, rhamnose, xylose and mannose in this polysaccharide were 72.09%, 19.07%, 6.93%, 0.86%, 0.52% and 0.52%, respectively.

Cell viability

IPEC-J2 cells were exposed to various concentrations (0, 1.5, 3.0, 6.0, 12.0 and 24.0 mg/L) of LNT polysaccharide for 6, 12 and 24 h. During 6 h, the IPEC-J2 cell viability was increased by 1.5 (P = 0.07), 3.0 (P < 0.05), 6.0 (P < 0.01) and 12.0 (P = 0.06) mg/L LNT polysaccharide (Table 4). During 12 h, the IPEC-J2 cell viability was only enhanced by 24.0 mg/L LNT polysaccharide (P < 0.05, Table 4). After 24 h incubation, 3.0–24.0 mg/L LNT polysaccharide, to some extent, increased IPEC-J2 cell viability, and 24.0 mg/L LNT polysaccharide improved 25.8% viability in IPEC-J2 cells (P < 0.01, Table 4). Thus, in the following experiments, the dose and duration of LNT polysaccharide exposure are 24.0 mg/L and 24 h, respectively.

Table 4

The effect of LNT polysaccharide on IPEC-J2 cell viability (%)
		Treatment duration (h)
		6	12	24
LNT polysaccharide (mg/L)	0.0	100.00 ± 2.21	100.00 ± 3.67	100.00 ± 2.35
	1.5	108.20 ± 3.44^#	92.25 ± 2.10	108.06 ± 3.17^#
	3.0	109.72 ± 3.57^*	102.99 ± 3.76	111.32 ± 3.31^*
	6.0	113.85 ± 1.47^**	94.53 ± 5.23	117.80 ± 1.24^**
	12.0	109.58 ± 3.36^#	102.99 ± 2.98	123.36 ± 1.44^**
	24.0	100.86 ± 2.15	115.51 ± 1.94^*	125.80 ± 1.22^**
^#P < 0.10, ^P < 0.05 and ^*P < 0.01, vs. 0 mg/L LNT polysaccharide group (n = 9).

As shown in Fig. 3, the viability of IPEC-J2 cells with or without LNT polysaccharide was decreased by RV infection, which was dependent with exposure duration. However, after IPEC-J2 cells were exposed to 24.0 mg/L of LNT polysaccharide for 24 h, the reduction of IPEC-J2 cell viability induced by RV challenge was alleviated in 2–4 d (P < 0.01 or P < 0.05, Fig. 3).

The NSP4 concentration in cell supernatants

With RV challenge, the RV NSP4 level in IPEC-J2 cell media was increased (P < 0.05, Fig. 4). However, the addition of LNT polysaccharide reduced the RV NSP4 concentration in IPEC-J2 cell media after RV infection (P < 0.05, Fig. 4).

The mRNA expressions of immune-relative genes in IPEC-J2 cells

The effects of LNT polysaccharide and RV infection on the mRNA expressions of immune-relative genes in IPEC-J2 cells were exhibited in Figs. 5 and 6. RV challenge stimulated retinoic acid inducible protein 1 (RIG-I), melanoma differentiation-associated protein 5 (MDA5), mitochondrial antiviral signaling protein (MAVS), IFN-β and interferon stimulated gene 15 (ISG-15) mRNA expressions (P < 0.05), and inhibited pBD1, pBD2 and pBD3 mRNA expressions (P < 0.05) in IPEC-J2 cells. However, LNT polysaccharide could improve pBD2 and pBD3 mRNA expressions (P < 0.05), and further up-regulate RIG-I, MDA5, MAVS, IFN-β and ISG-15 mRNA expressions (P < 0.05) in the RV-infected IPEC-J2 cells.

Diarrhea of piglets has been a major problem in the pig industry, which can lead to high mortality of piglets in china. RV is one of the main viral pathogens that cause diarrhea and death in piglets. Many experiments have found that RV can decrease growth performance, induce diarrhea, and impair immune function in children and young animals [22–24], which was consistent with the results of this study. In addition, our study also showed that RV infection increased RV-Ab levels in serum and jejunal mucosa of weaned pigs, and induced the decreasing cell viability and the increasing generation of NSP4 in IPEC-J2 cells. These results demonstrated that the in vivo and in vitro models of RV infection in weaned piglets and IPEC-J2 cells were successfully implemented, respectively.

In this study, dietary LNT supplementation could reduce F/G ratio of weaned pigs, which is consistent with previous studies [25]. And we also found that LNT relieved the effect of RV infection on ADG and F/G ratio of weaned pigs. The digestion, absorption and utilization of nutrients are important factors that affect growth performance, which, to some extent, can be evaluated by nutrient digestibility and blood urea nitrogen level. The previous study has shown that dietary LNT supplementation could increase the nutrient digestibility of pigs [26], which is consistent with the results of our study. Moreover, in the present study, supplementing LNT in diets might also alleviate the increasing SUN level in piglets with or without RV infection. Thus, it is possible that LNT promoting the growth of piglets should be relative with the increasing digestion, absorption and utilization of nutrients.

The gut health, especially integrity of intestinal epithelial cells, is very important to the digestion and absorption function. Our previous study has shown that dietary LNT supplementation improved the morphology of jejunal mucosa in the normal and RV-infected weaned pigs [12]. Moreover, in present study, we also found that LNT polysaccharide enhanced the viability of IPEC-J2 cells, and alleviated the negative effect of RV challenge on the viability of IPEC-J2 cells.

Concentrations of LNT polysaccharide ranged from 1.5 to 24.0 mg/L were chosen in our study, which are in accordance with the levels probably encountered in the gastrointestinal tract of piglets after feed intake in animal breeding experiments. Based on the water content in the digesta of small intestine and the recommending dose of LNT product (purity of polysaccharide is 30%) in feeds, the concentrations of LNT product should be 4.5–72.0 mg/L. Finally, the effective dose of LNT polysaccharide (purity of polysaccharide is 95%) in cell experiments was about 1.5–24.0 mg/L.

Immune function can withstand the pathogen, which is very pivotal to maintain gut health of animals and human. Many researches have reported that LNT can regulate immune function [27–29]. Immunoglobulin levels are the important index of humoral immunity [30]. In many in vivo and in vitro studies, LNT can regulate the immunoglobulin levels [31–34]. This study reported that dietary LNT supplementation could increase serum IgA, IgM and IgG levels in weaned piglets, and further increase IgM level of serum and RV-Ab level of serum and jejunal mucosa in RV-infected piglets. Besides these, we also found that LNT administration restored the effect of jejunal mucosal sIgA level, which is considered as the important content of intestinal immunologic barrier [35, 36]. These demonstrate that LNT improved the growth and health, at least partially, via increasing the humoral immunity of body.

As a kind of type I interferon, IFN-β can stimulate the productions of antiviral protein in cells via up-regulating expressions of some genes (such as ISG-15), which reduces virus replication. Then, it was considered as antiviral agents [37]. Under some disease condition (i.e. human immunodeficiency virus, HIV), LNT induces interferon secretion [38, 39]. The current study also showed that, under RV infection condition, LNT administration further increased the IFN-β level in the jejunal mucosa of weaned pigs, and the IFN-β and ISG-15 mRNA expressions in IPEC-J2 cells. Thus, dietary LNT supplementation alleviating RV-induced diarrhea could be associated with IFN-β-increasing antivirus capacity.

When RV invades gut and cells via pathogen-associated molecular pattern (PAMPs), these PAMPs are sensed by pattern recognition receptors (PRRs) of host [40]. These PRRs, such as Toll-like receptors (TLRs), RIG-I-like receptors, are expressed on many cells (including immune cells, epithelial cells) [41, 42]. Following PAMPs’ stimulation, host immunity (PRRs and their related signaling pathways) will be activated, and lead to the transcription and synthesis of many cytokines (especially, type I interferon). This will efficiently inhibit pathogen invasion [43]. In our study, RV infection, to some extent, up-regulated TLR3, RIG-I, MDA5 and MAVS mRNA expressions in IPEC-J2 cells, which may have induced the anti-viral immunity of host. Additionally, we also found that, in RV-infected IPEC-J2 cells, LNT polysaccharide further stimulated RIG-I, MDA5 and MAVS mRNA expressions, but did not affect TLR3 mRNA expression. These also showed that LNT polysaccharide increased anti-viral function of type I interferon via regulating RIG-I/MDA5/MAVS pathways, which could be not associated with TLR3 and its relative pathways.

Host defense peptides (HDPs) also are an important part of innate immunity, which are the barriers for preventing invasion of pathogens [44]. This process is derived from HDPs regulating inflammatory cytokines and responses via some signaling pathways, such as Toll-like receptors 4, mitogen-activated protein kinase and nuclear factor-kappa B signaling pathways [45–48]. And the HBD-2 mRNA expression in pulmonary epithelial cells could be induced by lentinan in a concentration- and time-dependent manner [49]. The current study showed that RV infection decreased the mRNA expressions of pBD1, pBD2 and pBD3 in jejunal mucosa of piglets and IPEC-J2 cells, but LNT administration relieved the effect of RV infection on pBD1, pBD2 and pBD3 mRNA expressions in jejunal mucosa of piglets, and pBD2 and pBD3 mRNA expressions in IPEC-J2 cells. About the different results between in vivo and in vitro trials, it was possibly derived from LNT contents and HDPs’ generation mechanisms. Thus, LNT improving immune function of RV-infected host could be due to the stimulation of HDPs in intestinal mucosa.

In summary, under normal or RV-challenge conditions, dietary LNT supplementation improved growth performance and decreased diarrhea, which could be due to the increase of nutrient digestibility and immunity in weaned pigs. Via purity of LNT polysaccharide and in vitro trials, LNT administration enhanced gut anti-viral immunity possibly via stimulating pattern recognition receptor pathway (such as RIG-I/MDA5/MAVS pathways) and HDPs’ generation. In addition, based on the similarity of physiology between pigs and humans, LNT may be used to prevent and cure rotavirus infection in young children.

LNT: lentinan; RV: rotavirus; TCID₅₀: tissue culture infective dose 50; ADG: average daily gain; ADFI: average daily feed intake; SUN: urea nitrogen; RV-Ab: rotavirus antibody; Ig: immunoglobulin; IFN-β: interferon-beta; Mw: molecular weight; HPGPC: high performance gel permeation chromatography; NSP4: non-structural protein 4; RIG-I: retinoic acid inducible protein 1; MDA5: melanoma differentiation-associated protein 5; ISG-15: interferon stimulated gene 15; MAVS: mitochondrial antiviral signaling protein.

Ethics approval

The animal care and experimental procedures were approved by the Institutional Animal Care and Use Committee of Sichuan Agricultural University (S20174302). All experimental procedures followed established guidelines for the care and handling of laboratory animals.

Consent for publication

Not applicable.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Competing interests

All authors declare that they have no competing interests.

Funding

This study was financially supported by the grant from the earmarked fund for the China Agriculture Research System (CARS-35), the fund from the Project of SAU-Adisseo Centre of Research on Nutrition and Health, and the grant from Science and Technology Support Project of Sichuan Province (2016NYZ0052 and 2016NZ0006).

Author statement

Conceptualization, X.M., D.C., B.Y. and J.H.; Funding acquisition, X.M., D.C. and E.E.; Methodology, J.Y., J.L. and Y.L.; Data analysis, J.W.; Project administration, H.H., X.F. and X.M.; Writing-original draft, H.H. and X.F.; Writing-review & editing, H.Y., X.M. and E.E.

Acknowledgments

Special thanks to Professor De Wu from Sichuan Agricultural University for editing the manuscript.

Jong SC, Birmingham JM. Medicinal and therapeutic value of the Shiitake mushroom. Adv Appl Microbiol. 1993;39:153-184.
Zhang Y, Li S, Wang X, Zhang L, Cheung PCK. Advances in lentinan: isolation, structure, chain conformation and bioactivities. Food Hydrocolloid. 2011;25:196-206.
Rincão VP, Yamamoto KA, Ricardo NMPS, Soares SA, Meirelles LDP, Nozawa C, Linhares REC. Polysaccharide and extracts from Lentinula edodes: structural features and antiviral activity. Virol J. 2012;9:
Ina D, Kataoka T, Ando T. The use of lentinan for treating gastric cancer. Anticancer Agents Med Chem. 2013;13:681-688.
Ahn H, Jeon E, Kim J, Kang SG, Yoon S, Ko H, Kim P, Lee G. Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production. Sci Rep. 2017;7:
Mao X, Chen D, Hu H, Yu B, Gu C, He J, Yu J, Luo J, Luo Y, Zheng P. Effects of lentinan supplementation on growth performance, antioxidant capacity and intestinal health of weaned Rats. Chin J Anim Nutr. 2017;29:859-865.
Anderson E.J, S.G. Weber. Rotavirus infection in adults. Lancet Infect. Dis. 2004;4:91-99.
Ramig RF. Pathogenesis of intestinal and systemic rotavirus infection. J Virol. 2004;78:10213-10220.
Jiang Y, Ye L, Cui Y, Yang G, Yang W, Wang J, Hu J, Gu W, Shi V, Huang H, Wang C. Effects of Lactobacillus rhamnosus GG on the maturation and differentiation of dendritic cells in rotavirus-infected mice. Benef Microbes. 2017;8:645-656.
Dennehy PH. Rotavirus vaccines: an overview. Clin Microbiol Rev. 2008;21:198-208.
Bocchini JA, Bradley JS, Brady MT, Bernstein HH, Rubin LG. Prevention of rotavirus disease: updated guidelines for use of rotavirus vaccine. 2009;123:1412-1420.
X Mao, H Hu, X Xiao, D Chen, B Yu, J He, J Yu, P Zheng, J Luo, Y Luo, J Wang. Lentinan administration relieves gut barrier dysfunction induced by rotavirus in a weaned piglet model. Food Funct. 2019;10:2094.
Diao H, Gao X, Yu B, Zheng P, He J, Yu J, Huang Z, Chen D, Mao X. Effects of benzoic acid (VevoVitall^®) on the performance and jejunal digestive physiology in young pigs. J Anim Sci Biotechnol. 2016;7:32.
Chen H, Hu H, Chen D, Tang J, Yu B, Luo J, He J, Luo Y, Yu J, Mao X. Dietary pectic oligosaccharide administration improves the growth performance and immunity in weaned pigs infected by rotavirus. J Agric Food Chem. 2017;65:2923-2929.
Mao X, Gu C, Ren M, Chen D, Yu B, He J, Yu J, Zheng P, Luo J, Luo Y, Wang J, Tian G, Yang Q. L-isoleucine administration alleviates rotavirus infection and immune response in the weaned piglet model. Front Immunol. 2018;9:1654.
Mao X, Liu M , Tang J, Chen H, Chen D, Yu B, He J, Yu J, Zheng P. Dietary leucine supplementation improves the mucin production in the jejunal mucosa of the weaned pigs challenged by porcine rotavirus. PLoS ONE. 2015;10:e0137380.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔ CT method. Methods. 2012;25:402-408.
Zeng BY, Su MH, Chen QX, Chang Q, Wang W, Li HH. Antioxidant and hepatoprotective activities of polysaccharides from Anoectochilus roxburghii. Carbohydr Polym. 2016;153:391-398.
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1856;28:350-356.
Liu Y, You Y, Li Y, Zhang L, Yin L, Shen Y, Li C, Chen H, Chen S, Hu B, Chen D. The characterization, selenylation and antidiabetic activity of mycelial polysaccharides from Catathelasma ventricosum. Carbohydr. Polym. 2017;174:72-81.
Liu J, Zhao YP, Wu QX, John A, Jiang YM, Yang JL, Liu HL, Yang B. Structure characterisation of polysaccharides in vegetable “okra” and evaluation of hypoglycemic activity. Food Chem. 2018;242:211-216.
Mao X, Gu C, Hu H, Tang , Chen D, Yu B, He J, Yu J, Luo J, Tian G. Dietary Lactobacillus rhamnosus GG supplementation improves the mucosal barrier function in the intestine of weaned piglets challenged by porcine rotavirus. PLoS ONE. 2016;11:
Mao X, Xiao X, Chen D, Yu B, He J, Chen H, Xiao X, Luo J, Luo Y, Tian G, Wang J. Dietary apple pectic oligosaccharide improves gut barrier function of rotavirus-challenged weaned pigs by increasing antioxidant capacity of enterocytes. Oncotarget. 2017;8:92420-92430.
Kawahara T, Makizaki Y, Oikawa Y, Tanaka Y, Maeda A, Shimakawa M, Komoto S, Moriguchi K, Ohno H, Taniguchi K. Oral administration of Bifidobacterium bifidum G9-1 alleviates rotavirus gastroenteritis through regulation of intestinal homeostasis by inducing mucosal protective factors. PLoS ONE. 2017;12:
Liu D, ZhuH, Dong H, Wang L, Zhao Y, Zhao J, Shi G. Effect of Lentinus edodes polysaccharide on the growth of weaned piglets. Tianjin Sci Technol. 2014;41:92-93, 97.
Xue L, Li T, Zang S, Xi Y, Yuan N, Li Q. Effects of Lentinus edodes polysaccharide on growth performance, nutrient digestibility and antioxidant ability in piglets. Anim Husbandry Vet Med 2009;41:5-9.
Herlyn D, Kaneko Y, Powe J, Aoki T, Koprowski H. Stimulation of monoclonal antibody-dependent macrophage-mediated cytotoxicity against human tumors by lentinan. Int J Immunopharmacol. 1985;7:332.
Liu Q, Dong L, Li H, Yuan J, Peng Y, Dai S. Lentinan mitigates therarubicin-induced myelosuppression by activating bone marrow-derived macrophages in an MAPK/NF-κB-dependent manner. Oncol Rep. 2016;36:315-323.
Meng Q, Xia Y. Effect and clinical significance of TACE combined with Lentinan on T-lymphocyte subsets, IL-12 and sIL-2R in patients with hepatic cancer. J Taishan Med Coll. 2010;31:413-415.
Luo Q, Cui H, Peng X, Fang J, Zuo Z, Deng J, Liu J, Deng Y. Intestinal IgA⁺ cell numbers as well as IgA, IgG, and IgM contents correlate with mucosal humoral immunity of broilers during supplementation with high fluorine in the diets. Biol. Trace Elem Res. 2013;154:62-72.
Miyakoshi H, Aoki T. Acting mechanisms of Lentinan in human--I. Augmentation of DNA synthesis and immunoglobulin production of peripheral mononuclear cells. Int J Immunopharmacol. 1984;6:365-371.
Zhang B, Guo Y, Wang Z. The modulating effect of beta-1,3/1,6-glucan supplementation in the diet on performance and immunological responses of broiler chickens. Asian-Australas. J Anim Sci. 2008;21:237-244.
Li T, Xue L, Zang S, Li Q, Xi Y, Yuan N. Effects of Lentinus edodes polysaccharide on growth performance, some serum hormone and immune indices in piglets. Chin. J Anim Nutr 2009;21:101-106.
Wu S, Cheng S, Tang F, Li A, Kong X. Effects of dietary additive of mushroom polysaccharides on immune function in early-weaned piglets and study on mechanism. J Chin. Cereals Oil Assoc. 2009;24:113-118.
Bakker-Zierikzee AM, Van Tol EAF, Kroes H, Alles MS, Kok FJ, Bindels JG. Faecal sIgA secretion in infants fed on pre- or probiotic infant formula. Pediatr Allergy Immunol. 2006;17:134-140.
Blikslager AT, Moeser AJ, Gookin JL, Jones SL, Odle J. Restoration of barrier function in injured intestinal mucosa. Physiol Rev. 2007;87:545-564.
Farias MVN, Lendez PA, Marin M, Quintana S, Martínez-Cuesta L, Ceriani MC, Dolcini GL. Toll-like receptors, IFN-γ and IL-12 expression in bovine leukemia virus-infected animals with low or high proviral load. Res Vet Sci. 2016;107:190-195.
Yoshida O, Nakashima H, Yoshida T, Kaneko Y, Yamamoto I, Matsuzaki K, Uryu T, Yamamoto N. Sulfation of the immunomodulating polysaccharide lentinan: a novel strategy for antivirals to human immunodeficiency virus (HIV). Biochem Pharmacol. 1988;37:2887-2891.
Li D, Shang H, Jiang Y, Wang Y. In vitro immune regulatory effect of lentinan on HIV-infected PBMC. Chin J Immunol. 2004;20:253-255.
Janeway CA, Jr, Medzhitov R. Innate immune recognition. Annu Rev Immunol. 2002;20:197-216.
Medzhitov R. Recognition of microorganisms and activation of the immune response. Nature. 2007;449:819-826.
Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on toll-like receptors. Nat Immunol. 2010;11:373-384.
Kumar H, Kawai T, Akira S. Pathogen recognition in the innate immune response. Biochem J. 2009;420:1-16.
Zhao J, Sun Y, Li J, Li Z. Antiviral function of animal antimicrobial peptides. J Med Mol Biol. 2008;296:1044-1050.
Mookherjee N, Brown KL, Bowdish DME, Doria S, Falsafi R, Hokamp K, Roche FM, Mu R, Doho G.H, Pistolic J, Powers J, Bryan J, Brinkman FSL, Hancock REW. Modulation of the TLR-mediated inflammatory response by the endogenous human host defense peptide LL-37. J Immunol. 2006;176:2455-2464.
Li G, Domenico J, Jia Y, Lucas JJ, Gelfand E.W. NF-kappaB-dependent induction of cathelicidin-related antimicrobial peptide in murine mast cells by lipopolysaccharide. Int Arch Allergy Immunol. 2009;150:122-132.
Kiatsurayanon C, Niyonsaba F, Chieosilapatham P, Okumura K, Ikeda S, Ogawa H. Angiogenic peptide (AG)-30/5C activates human keratinocytes to produce cytokines/chemokines and to migrate and proliferate via MrgX receptors. J Dermatol Sci. 2016;83:190-199.
Kanazawa K, Okumura K, Ogawa H, Niyonsaba F. An antimicrobial peptide with angiogenic properties, AG-30/5C, activates human mast cells through the MAPK and NF-κB pathways. Immunol Res. 2016;64:594-603.
Zhou Y, Dong B, Teng L, Wang H. Lentinan induces the expression of human-β-defensin-2 in pulmonary epithelial cells. J Biomed Eng. 2009;26:1311-1314.

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Lentinan Administration Alleviates Diarrhea of Rotavirus-Infected Weaned Pigs via Regulating Intestinal Immunity

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Abstract

Figures

Background

Methods

Animal trial

Animals and diets

Experimental design and sample collection

Analysis of nutrient digestibility

Analysis of urea nitrogen (SUN), rotavirus antibody (RV-Ab), immunoglobulin (Ig) and/or interferon-beta (IFN-β) levels in serum and jejunal mucosa

Analysis of expressions of host defense peptides in the jejunal mucosa

Extraction and analysis of LNT polysaccharides

Preparation of water-soluble polysaccharides

Molecular weight analysis

Monosaccharide composition

Cell cultural trial

Cell culture

Cell viability assay

Cell experiments

Analysis of rotavirus non-structural protein 4 (NSP4) in cell supernatants

Analysis of gene expressions in IPEC-J2 cells

Statistical analysis

Results

Growth performance, SUN, and nutrient digestibility of weaned pigs

The diarrhea rate and RV-Ab levels of serum and jejunal mucosa in weaned pigs

Serum immunoglobulin concentrations, and sIgA and IFN-β levels and host defense peptide expressions of jejunal mucosa in weaned pigs

Primary characteristics of LNT polysaccharides

Cell viability

The NSP4 concentration in cell supernatants

The mRNA expressions of immune-relative genes in IPEC-J2 cells

Discussion

Conclusions

Abbreviations

Declarations

References

Supplementary Files

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