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Research
Lentinan Administration Alleviates Diarrhea of Rotavirus-Infected Weaned Pigs via Regulating Intestinal Immunity
Xiangbing Mao
Sichuan Agricultural University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background
This study aimed to verify whether lentinan (LNT) administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs.
Methods
Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15.
Results
Dietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P < 0.05). The polysaccharide was isolated from LNT product, whose molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infected IPEC-J2 cells, this polysaccharide significantly increased cell viability (P < 0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P < 0.05).
Conclusion
Those results suggest that LNT administration increases the piglets’ resistance to RV-induced stress, likely by supporting intestinal immunity.
Keywords
lentinan, weaned pigs, IPEC-J2 cells, rotavirus, gut immunity
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Peer Review Timeline
CURRENT STATUS: Under Review
Version (no preprint)
Posted 16 Dec, 2020
Editorial decision: Minor revision
On 16 Dec, 2020
Review #1 received
Received 13 Dec, 2020
Reviewers invited
Invitations sent on 12 Dec, 2020
Reviewer #1 agreed
On 12 Dec, 2020
Editor assigned
On 09 Dec, 2020
Submission checks complete
On 09 Dec, 2020
Editor invited
On 09 Dec, 2020
This version was not preprinted
Version 1
Posted 18 Sep, 2020
No community comments so far
Review #2 received
Received 15 Nov, 2020
Editorial decision: Major revision
On 15 Nov, 2020
Reviewer #2 agreed
On 01 Nov, 2020
Review #1 received
Received 15 Oct, 2020
Reviewer #1 agreed
On 02 Oct, 2020
Reviewers invited
Invitations sent on 17 Sep, 2020
Editor assigned
On 16 Sep, 2020
First submitted
On 15 Sep, 2020
Submission checks complete
On 15 Sep, 2020
Editor invited
On 15 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Animal Science
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This preprint is under consideration at Journal of Animal Science and Biotechnology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Lentinan Administration Alleviates Diarrhea of Rotavirus-Infected Weaned Pigs via Regulating Intestinal Immunity
Xiangbing Mao
Sichuan Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version (no preprint)
Posted 16 Dec, 2020
Editorial decision: Minor revision
On 16 Dec, 2020
Review #1 received
Received 13 Dec, 2020
Reviewers invited
Invitations sent on 12 Dec, 2020
Reviewer #1 agreed
On 12 Dec, 2020
Editor assigned
On 09 Dec, 2020
Submission checks complete
On 09 Dec, 2020
Editor invited
On 09 Dec, 2020
This version was not preprinted
Version 1
Posted 18 Sep, 2020
No community comments so far
Review #2 received
Received 15 Nov, 2020
Editorial decision: Major revision
On 15 Nov, 2020
Reviewer #2 agreed
On 01 Nov, 2020
Review #1 received
Received 15 Oct, 2020
Reviewer #1 agreed
On 02 Oct, 2020
Reviewers invited
Invitations sent on 17 Sep, 2020
Editor assigned
On 16 Sep, 2020
First submitted
On 15 Sep, 2020
Submission checks complete
On 15 Sep, 2020
Editor invited
On 15 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Animal Science
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
This study aimed to verify whether lentinan (LNT) administration could relieve diarrhea via improving gut immunity in rotavirus (RV)-challenged weaned pigs.
Methods
Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d (n = 14). RV infection was executed on d 15.
Results
Dietary LNT supplementation supported normal function of piglets even when infected with RV, as reflected by reduced growth performance loss and diarrhea prevalence, and maintained gut immunity (P < 0.05). The polysaccharide was isolated from LNT product, whose molecular weight was 5303 Da, and major monosaccharides included glucose, arabinose and galactose. In RV-infected IPEC-J2 cells, this polysaccharide significantly increased cell viability (P < 0.05), and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides (P < 0.05).
Conclusion
Those results suggest that LNT administration increases the piglets’ resistance to RV-induced stress, likely by supporting intestinal immunity.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6