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Short communication
Effect of porcine immature oocyte vitrification on oocyte-cumulus cell gap junctional intercellular communication.
Fahiel Casillas
Universidad Autonoma Metropolitana Iztapalapa
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9916-0586
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Porcine Health Management.
Vitrification may severely affect cumulus cells and oocyte morphology and viability, limiting their maturation and developmental potential. The aim of this study was to evaluate the gap junction intercellular communication (GJIC) integrity after the vitrification of porcine immature cumulus-oocyte complexes (COCs). Fresh COCs were randomly distributed in two groups: untreated (control) and vitrified (vitrification), then subjected to in vitro maturation (IVM). Oocyte viability and IVM were measured in both groups. The evaluation of GJIC was expressed as relative fluorescence intensity (RFI). Vitrification significantly decreased oocyte viability and maturation after 44 h of culture compared to control. Also, significantly reduced RFI was observed in vitrified COCs at 4 and 8 h of culture compared to control. This study demonstrates that porcine oocyte viability and maturation after 44 h of culture were compromised after vitrification. GJIC was also affected after the vitrification of GV oocytes, being the possible mechanism by which oocyte maturation decreased.
Keywords
vitrification, porcine, oocyte, viability, maturation, gap junctions
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On 25 Nov, 2020
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Received 21 Sep, 2020
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This preprint is under consideration at Porcine Health Management. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Short communication
Effect of porcine immature oocyte vitrification on oocyte-cumulus cell gap junctional intercellular communication.
Fahiel Casillas
Universidad Autonoma Metropolitana Iztapalapa
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9916-0586
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 17 Sep, 2020
Published
On 25 Nov, 2020
No community comments so far
Review #2 received
Received 04 Oct, 2020
Editorial decision: Major revision
On 04 Oct, 2020
Reviewer #2 agreed
On 03 Oct, 2020
Review #1 received
Received 21 Sep, 2020
Reviewers invited
Invitations sent on 15 Sep, 2020
Reviewer #1 agreed
On 15 Sep, 2020
Editor assigned
On 14 Sep, 2020
Submission checks complete
On 13 Sep, 2020
Editor invited
On 13 Sep, 2020
First submitted
On 12 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Animal Science
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Porcine Health Management.
Vitrification may severely affect cumulus cells and oocyte morphology and viability, limiting their maturation and developmental potential. The aim of this study was to evaluate the gap junction intercellular communication (GJIC) integrity after the vitrification of porcine immature cumulus-oocyte complexes (COCs). Fresh COCs were randomly distributed in two groups: untreated (control) and vitrified (vitrification), then subjected to in vitro maturation (IVM). Oocyte viability and IVM were measured in both groups. The evaluation of GJIC was expressed as relative fluorescence intensity (RFI). Vitrification significantly decreased oocyte viability and maturation after 44 h of culture compared to control. Also, significantly reduced RFI was observed in vitrified COCs at 4 and 8 h of culture compared to control. This study demonstrates that porcine oocyte viability and maturation after 44 h of culture were compromised after vitrification. GJIC was also affected after the vitrification of GV oocytes, being the possible mechanism by which oocyte maturation decreased.
Figure 1
Figure 2
Figure 3