Effect of porcine immature oocyte vitrification on oocyte-cumulus cell gap junctional intercellular communication.
Vitrification may severely affect cumulus cells and oocyte morphology and viability, limiting their maturation and developmental potential. The aim of this study was to evaluate the gap junction intercellular communication (GJIC) integrity after the vitrification of porcine immature cumulus-oocyte complexes (COCs). Fresh COCs were randomly distributed in two groups: untreated (control) and vitrified (vitrification), then subjected to in vitro maturation (IVM). Oocyte viability and IVM were measured in both groups. The evaluation of GJIC was expressed as relative fluorescence intensity (RFI). Vitrification significantly decreased oocyte viability and maturation after 44 h of culture compared to control. Also, significantly reduced RFI was observed in vitrified COCs at 4 and 8 h of culture compared to control. This study demonstrates that porcine oocyte viability and maturation after 44 h of culture were compromised after vitrification. GJIC was also affected after the vitrification of GV oocytes, being the possible mechanism by which oocyte maturation decreased.
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Posted 17 Sep, 2020
On 25 Nov, 2020
Received 04 Oct, 2020
On 04 Oct, 2020
On 03 Oct, 2020
Received 21 Sep, 2020
Invitations sent on 15 Sep, 2020
On 15 Sep, 2020
On 14 Sep, 2020
On 13 Sep, 2020
On 13 Sep, 2020
On 12 Sep, 2020
Effect of porcine immature oocyte vitrification on oocyte-cumulus cell gap junctional intercellular communication.
Posted 17 Sep, 2020
On 25 Nov, 2020
Received 04 Oct, 2020
On 04 Oct, 2020
On 03 Oct, 2020
Received 21 Sep, 2020
Invitations sent on 15 Sep, 2020
On 15 Sep, 2020
On 14 Sep, 2020
On 13 Sep, 2020
On 13 Sep, 2020
On 12 Sep, 2020
Vitrification may severely affect cumulus cells and oocyte morphology and viability, limiting their maturation and developmental potential. The aim of this study was to evaluate the gap junction intercellular communication (GJIC) integrity after the vitrification of porcine immature cumulus-oocyte complexes (COCs). Fresh COCs were randomly distributed in two groups: untreated (control) and vitrified (vitrification), then subjected to in vitro maturation (IVM). Oocyte viability and IVM were measured in both groups. The evaluation of GJIC was expressed as relative fluorescence intensity (RFI). Vitrification significantly decreased oocyte viability and maturation after 44 h of culture compared to control. Also, significantly reduced RFI was observed in vitrified COCs at 4 and 8 h of culture compared to control. This study demonstrates that porcine oocyte viability and maturation after 44 h of culture were compromised after vitrification. GJIC was also affected after the vitrification of GV oocytes, being the possible mechanism by which oocyte maturation decreased.
Figure 1
Figure 2
Figure 3