See the published version of this article at BMC Biotechnology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
The Osteogenic Differentiation of Mesenchymal Stem Cells Derived From Dental Pulp in Modular Alginate-gelatin/nano-hydroxyapatite Microcapsules
Zahra Aghazadeh
Daneshgah St, Golgasht St, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Biotechnology.
Background
Microcapsule is considered as a promising 3D microenvironment for Bone Tissue Engineering (BTE) applications. Microencapsulation of cells in an appropriate scaffold not only protects cells against excess stress but also promotes cell proliferation and differentiation.
Results
The SEM results revealed that Alg/Gel microcapsules containing nHA showed a rough and more compact surface morphology in comparison with the Alg/Gel microcapsules. Moreover, microencapsulation by Alg/Gel/nHA could improve cell proliferation and induce osteogenic differentiation. The cells cultured in the Alg/Gel and Alg/Gel/nHA microcapsules after 21 days showed 1.4-fold and 1.7-fold more activity of BMP-2 gene expression in comparison with the cells cultured on the polystyrene surface as a control group. These amounts for the BMP-2 gene were 2.5-fold and 4-fold more expression for the Alg/Gel and Alg/Gel/nHA microcapsules after 28 days. The nHA addition to hDPSCs-laden Alg/Gel microcapsule could also up-regulate the bone-related gene expressions of osteocalcin, osteonectin, and RUNX-2 during 21 and 28 days culture period. Calcium deposition and ALP activities of the cells were observed in accordance with the proliferation results as well as the gene expression analysis.
Conclusion
The study demonstrated that microencapsulation of hDPSCs inside Alg/Gel/nHA hydrogel can be a potential approach for regenerative dentistry in the near future.
Keywords
Alginate-gelatin microcapsules, Bone Tissue Engineering (BTE), Human Dental Pulp Stem Cells (hDPSCs), Nano-hydroxyapatite
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 18 Sep, 2020
Published
On 11 Jan, 2021
No community comments so far
Editorial decision: Major revision
On 19 Oct, 2020
Review #1 received
Received 17 Oct, 2020
Review #2 received
Received 14 Oct, 2020
Reviewer #2 agreed
On 12 Oct, 2020
Reviewer #1 agreed
On 30 Sep, 2020
Reviewers invited
Invitations sent on 29 Sep, 2020
Editor assigned
On 25 Sep, 2020
Submission checks complete
On 16 Sep, 2020
Editor invited
On 16 Sep, 2020
First submitted
On 13 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
Learn more about our company.
This preprint is under consideration at BMC Biotechnology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
The Osteogenic Differentiation of Mesenchymal Stem Cells Derived From Dental Pulp in Modular Alginate-gelatin/nano-hydroxyapatite Microcapsules
Zahra Aghazadeh
Daneshgah St, Golgasht St, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 18 Sep, 2020
Published
On 11 Jan, 2021
No community comments so far
Editorial decision: Major revision
On 19 Oct, 2020
Review #1 received
Received 17 Oct, 2020
Review #2 received
Received 14 Oct, 2020
Reviewer #2 agreed
On 12 Oct, 2020
Reviewer #1 agreed
On 30 Sep, 2020
Reviewers invited
Invitations sent on 29 Sep, 2020
Editor assigned
On 25 Sep, 2020
Submission checks complete
On 16 Sep, 2020
Editor invited
On 16 Sep, 2020
First submitted
On 13 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Biotechnology.
Background
Microcapsule is considered as a promising 3D microenvironment for Bone Tissue Engineering (BTE) applications. Microencapsulation of cells in an appropriate scaffold not only protects cells against excess stress but also promotes cell proliferation and differentiation.
Results
The SEM results revealed that Alg/Gel microcapsules containing nHA showed a rough and more compact surface morphology in comparison with the Alg/Gel microcapsules. Moreover, microencapsulation by Alg/Gel/nHA could improve cell proliferation and induce osteogenic differentiation. The cells cultured in the Alg/Gel and Alg/Gel/nHA microcapsules after 21 days showed 1.4-fold and 1.7-fold more activity of BMP-2 gene expression in comparison with the cells cultured on the polystyrene surface as a control group. These amounts for the BMP-2 gene were 2.5-fold and 4-fold more expression for the Alg/Gel and Alg/Gel/nHA microcapsules after 28 days. The nHA addition to hDPSCs-laden Alg/Gel microcapsule could also up-regulate the bone-related gene expressions of osteocalcin, osteonectin, and RUNX-2 during 21 and 28 days culture period. Calcium deposition and ALP activities of the cells were observed in accordance with the proliferation results as well as the gene expression analysis.
Conclusion
The study demonstrated that microencapsulation of hDPSCs inside Alg/Gel/nHA hydrogel can be a potential approach for regenerative dentistry in the near future.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6