In this study we are reporting a prediction model for the estimation of carbon dioxide (CO2) fixation based on the Adaptive Neuro-Fuzzy Inference System (ANFIS) and Genetic Algorithm (GA) hybrid approach. The experimental parameters such as temperature and pH conditions of the micro-algae-based carbon dioxide uptake process were taken as the input variables and the CO2 fixation rate was taken as the output variable. The optimization of ANFIS parameters and formation of the model structure were performed by genetic algorithm (GA) algorithm in order to achieve optimum prediction capability and industrial applicability. The best-fitting model was figured out using statistical analysis parameters such as RMSE, R2 and AARD. According to the analysis, GA-ANFIS model depicted a superior prediction capability over ANFIS optimized model. The Root Mean Square Error (RMSE), coefficient of determination (R2) and AARD for GA-ANFIS were determined as 0.000431, 0.97865 and 0.044354 in the training phase and 0.00056, 0.98457 and 0.032156 in the testing phase, respectively for the GA-ANFIS Model. As a result, it can be concluded that the proposed GA-ANFIS model is an efficient technique having very high potential to accurately calculate CO2 fixation rate and the exploration of the industrial scale-up process for commercial activities.